Clostridium phage HM2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 7.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C3S7G5|A0A1C3S7G5_9CAUD Uncharacterized protein OS=Clostridium phage HM2 OX=1874333 PE=4 SV=1
MM1 pKa = 7.57LKK3 pKa = 10.09LHH5 pKa = 6.3YY6 pKa = 9.78IIPGYY11 pKa = 10.3KK12 pKa = 9.26IIKK15 pKa = 5.4TTSNYY20 pKa = 9.35KK21 pKa = 10.33PNVGDD26 pKa = 3.7AVVLIITEE34 pKa = 4.33YY35 pKa = 8.83IVTYY39 pKa = 8.7ITYY42 pKa = 10.07DD43 pKa = 3.21INNDD47 pKa = 3.38IINIYY52 pKa = 10.32CEE54 pKa = 4.07EE55 pKa = 4.25KK56 pKa = 10.56EE57 pKa = 4.1

Molecular weight: 6.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1C3S7V0|A0A1C3S7V0_9CAUD Uncharacterized protein OS=Clostridium phage HM2 OX=1874333 PE=4 SV=1
MM1 pKa = 6.84CTNRR5 pKa = 11.84GHH7 pKa = 6.83LRR9 pKa = 11.84NMSSVRR15 pKa = 11.84IHH17 pKa = 7.12DD18 pKa = 3.71RR19 pKa = 11.84GGEE22 pKa = 4.03YY23 pKa = 10.12IPNNGNKK30 pKa = 9.79

Molecular weight: 3.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

217

28

68

43.4

5.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

1.843 ± 0.62

0.922 ± 0.554

3.226 ± 0.686

5.991 ± 1.758

3.687 ± 1.472

4.147 ± 1.703

4.147 ± 1.19

13.364 ± 2.09

8.756 ± 3.357

8.756 ± 3.217

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

4.147 ± 0.892

7.834 ± 1.745

2.765 ± 0.496

1.382 ± 0.988

5.991 ± 3.14

5.069 ± 1.775

5.991 ± 0.932

4.608 ± 0.868

0.922 ± 0.463

6.452 ± 1.695
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski