Dialister sp. CAG:588
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1171 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R7PN08|R7PN08_9FIRM Transcription termination/antitermination protein NusG OS=Dialister sp. CAG:588 OX=1262871 GN=nusG PE=3 SV=1
MM1 pKa = 7.48 NPTLFFSLSVDD12 pKa = 5.08 FIPDD16 pKa = 3.47 MQDD19 pKa = 2.59 GRR21 pKa = 11.84 QSFIEE26 pKa = 4.07 KK27 pKa = 9.39 MKK29 pKa = 9.79 EE30 pKa = 3.65 TATLYY35 pKa = 10.11 QSPFSFDD42 pKa = 3.16 AKK44 pKa = 10.57 DD45 pKa = 3.13 RR46 pKa = 11.84 PTIEE50 pKa = 5.3 KK51 pKa = 8.52 ITSANLLKK59 pKa = 10.37 ILPTLDD65 pKa = 3.71 LSAFGTWPSCILTCTIQSNHH85 pKa = 4.65 TVYY88 pKa = 10.8 SIGMSDD94 pKa = 2.98 GWDD97 pKa = 2.82 IYY99 pKa = 10.74 IGNKK103 pKa = 9.74 NFFAFAQFPADD114 pKa = 3.17 ALQIMEE120 pKa = 4.76 EE121 pKa = 3.96 ACLYY125 pKa = 9.93 YY126 pKa = 10.17 INNTEE131 pKa = 3.93 NQSFQEE137 pKa = 4.28 FVAQIGFDD145 pKa = 3.78 SFRR148 pKa = 11.84 DD149 pKa = 3.96 TILGNINPSTNAQSMHH165 pKa = 6.54 IDD167 pKa = 3.18 NPAFNLKK174 pKa = 10.25 EE175 pKa = 3.93 GDD177 pKa = 3.84 FVRR180 pKa = 11.84 PEE182 pKa = 3.97 HH183 pKa = 7.01 NIMQIIDD190 pKa = 4.39 VYY192 pKa = 11.16 PDD194 pKa = 3.2 MAPFLMEE201 pKa = 3.98 YY202 pKa = 11.0 GMSCVGCFISYY213 pKa = 10.36 EE214 pKa = 4.04 EE215 pKa = 4.57 NLWQGAQSHH224 pKa = 6.07 GLDD227 pKa = 3.18 VFEE230 pKa = 5.78 ILGEE234 pKa = 4.07 MNEE237 pKa = 4.4 YY238 pKa = 10.29 IADD241 pKa = 3.91 KK242 pKa = 10.64 YY243 pKa = 9.38 NKK245 pKa = 9.72 KK246 pKa = 10.39 VISKK250 pKa = 6.89 EE251 pKa = 4.14 TPMEE255 pKa = 4.83 DD256 pKa = 4.08 IITLYY261 pKa = 10.04 PQLLGILQSFHH272 pKa = 8.71 LEE274 pKa = 4.14 MPADD278 pKa = 3.67 MQTPLGAICNKK289 pKa = 10.43 ANVNVNDD296 pKa = 4.69 VINACDD302 pKa = 3.54 DD303 pKa = 3.52 KK304 pKa = 11.56 LRR306 pKa = 11.84 GVSEE310 pKa = 4.02 II311 pKa = 4.08
Molecular weight: 35.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.065
IPC2_protein 4.266
IPC_protein 4.228
Toseland 4.05
ProMoST 4.355
Dawson 4.202
Bjellqvist 4.342
Wikipedia 4.101
Rodwell 4.062
Grimsley 3.948
Solomon 4.19
Lehninger 4.151
Nozaki 4.304
DTASelect 4.507
Thurlkill 4.062
EMBOSS 4.113
Sillero 4.342
Patrickios 2.613
IPC_peptide 4.202
IPC2_peptide 4.329
IPC2.peptide.svr19 4.256
Protein with the highest isoelectric point:
>tr|R7PRL9|R7PRL9_9FIRM 50S ribosomal protein L23 OS=Dialister sp. CAG:588 OX=1262871 GN=rplW PE=3 SV=1
MM1 pKa = 7.75 AKK3 pKa = 10.54 GKK5 pKa = 7.67 MTYY8 pKa = 9.74 QPNNLWRR15 pKa = 11.84 KK16 pKa = 6.7 RR17 pKa = 11.84 THH19 pKa = 6.17 GFRR22 pKa = 11.84 ARR24 pKa = 11.84 MKK26 pKa = 8.69 TRR28 pKa = 11.84 AGRR31 pKa = 11.84 IVLKK35 pKa = 10.39 RR36 pKa = 11.84 RR37 pKa = 11.84 RR38 pKa = 11.84 AKK40 pKa = 9.89 GRR42 pKa = 11.84 KK43 pKa = 8.67 VLSAA47 pKa = 4.05
Molecular weight: 5.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.414
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.384
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.106
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.021
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1171
0
1171
338624
29
1455
289.2
32.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.329 ± 0.094
1.214 ± 0.027
4.991 ± 0.06
6.912 ± 0.076
4.069 ± 0.052
6.976 ± 0.071
2.134 ± 0.036
8.489 ± 0.075
7.118 ± 0.062
9.223 ± 0.071
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.033 ± 0.03
4.388 ± 0.054
3.341 ± 0.04
3.332 ± 0.039
4.287 ± 0.055
6.085 ± 0.057
5.503 ± 0.047
6.925 ± 0.053
0.965 ± 0.027
3.68 ± 0.05
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here