Escherichia phage vB_EcoP_PhAPEC5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Enquatrovirinae; Gamaleyavirus; Escherichia virus APEC5

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 83 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A067Y147|A0A067Y147_9CAUD RIIA-like protein OS=Escherichia phage vB_EcoP_PhAPEC5 OX=1395983 GN=PhAPEC5_34 PE=4 SV=1
MM1 pKa = 6.92SQEE4 pKa = 3.83DD5 pKa = 3.77ALFIIRR11 pKa = 11.84NFLDD15 pKa = 3.97ANWEE19 pKa = 4.08QFSEE23 pKa = 4.73FCEE26 pKa = 4.12EE27 pKa = 4.06LGEE30 pKa = 4.47SAEE33 pKa = 4.19EE34 pKa = 4.36TYY36 pKa = 10.84EE37 pKa = 4.04ATSGG41 pKa = 3.41

Molecular weight: 4.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A067Y083|A0A067Y083_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoP_PhAPEC5 OX=1395983 GN=PhAPEC5_10 PE=4 SV=1
MM1 pKa = 7.67RR2 pKa = 11.84KK3 pKa = 9.51SLIMGTKK10 pKa = 9.64EE11 pKa = 3.63DD12 pKa = 3.52VSRR15 pKa = 11.84LRR17 pKa = 11.84NRR19 pKa = 11.84NVASKK24 pKa = 9.46RR25 pKa = 11.84TLSMTNEE32 pKa = 3.84QLQEE36 pKa = 4.45FIDD39 pKa = 4.23AGLKK43 pKa = 8.89QSKK46 pKa = 10.23ARR48 pKa = 11.84IVTYY52 pKa = 9.03NHH54 pKa = 6.82PCIKK58 pKa = 10.55

Molecular weight: 6.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

83

0

83

22016

39

3571

265.3

29.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.78 ± 0.592

0.922 ± 0.178

5.759 ± 0.14

6.414 ± 0.277

3.702 ± 0.177

6.781 ± 0.247

1.699 ± 0.158

5.682 ± 0.279

6.314 ± 0.291

8.23 ± 0.191

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.989 ± 0.15

5.841 ± 0.201

3.988 ± 0.157

4.374 ± 0.292

4.242 ± 0.139

6.245 ± 0.24

6.368 ± 0.265

6.786 ± 0.222

1.204 ± 0.128

3.679 ± 0.266
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski