Goose adenovirus 4
Average proteome isoelectric point is 6.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 34 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I3PMP4|I3PMP4_9ADEN Protein ORF20A OS=Goose adenovirus 4 OX=1193422 GN=ORF20A PE=4 SV=1
MM1 pKa = 7.78 AEE3 pKa = 4.46 GGDD6 pKa = 3.53 PPRR9 pKa = 11.84 PNEE12 pKa = 4.67 GEE14 pKa = 4.98 DD15 pKa = 3.18 IQNDD19 pKa = 3.64 MEE21 pKa = 4.91 EE22 pKa = 4.17 IRR24 pKa = 11.84 ALLNDD29 pKa = 3.6 INVDD33 pKa = 3.54 ANGGDD38 pKa = 3.78 EE39 pKa = 4.31 DD40 pKa = 4.95 EE41 pKa = 5.07 EE42 pKa = 4.11 MLEE45 pKa = 4.09 FDD47 pKa = 5.99 DD48 pKa = 4.11 MQLMCFTNHH57 pKa = 6.75 EE58 pKa = 4.35 IVLTPCDD65 pKa = 3.41 CHH67 pKa = 5.88 QWYY70 pKa = 9.81 IRR72 pKa = 11.84 DD73 pKa = 3.56 FCMSLHH79 pKa = 6.06 SCIEE83 pKa = 3.99 SSVYY87 pKa = 8.63 PTALQLLGDD96 pKa = 4.34 DD97 pKa = 5.13 PGLCGCYY104 pKa = 9.7 FVTSNLGSMFALLNPVFMDD123 pKa = 3.71 RR124 pKa = 11.84 TIGSSNGVLIAVHH137 pKa = 5.94 EE138 pKa = 4.48 NNRR141 pKa = 11.84 HH142 pKa = 4.18 VFLGALCSVAEE153 pKa = 4.46 MGTMPCEE160 pKa = 3.88 YY161 pKa = 9.09 MRR163 pKa = 11.84 LVMMVGEE170 pKa = 4.49 RR171 pKa = 11.84 RR172 pKa = 11.84 VSMSCPRR179 pKa = 11.84 WMYY182 pKa = 10.77 YY183 pKa = 9.92 FVQLMSHH190 pKa = 7.27 FYY192 pKa = 10.85 YY193 pKa = 10.09 DD194 pKa = 3.61 TVDD197 pKa = 4.32 KK198 pKa = 10.54 LTGLNN203 pKa = 3.61
Molecular weight: 23.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.062
IPC2_protein 4.202
IPC_protein 4.151
Toseland 3.973
ProMoST 4.291
Dawson 4.126
Bjellqvist 4.279
Wikipedia 4.024
Rodwell 3.986
Grimsley 3.884
Solomon 4.113
Lehninger 4.075
Nozaki 4.24
DTASelect 4.431
Thurlkill 3.999
EMBOSS 4.037
Sillero 4.279
Patrickios 0.388
IPC_peptide 4.126
IPC2_peptide 4.253
IPC2.peptide.svr19 4.17
Protein with the highest isoelectric point:
>tr|I3PMN2|I3PMN2_9ADEN Core protein pX OS=Goose adenovirus 4 OX=1193422 GN=pX PE=4 SV=1
MM1 pKa = 7.74 SILISPSDD9 pKa = 3.51 NRR11 pKa = 11.84 GWGTMMRR18 pKa = 11.84 RR19 pKa = 11.84 SRR21 pKa = 11.84 SRR23 pKa = 11.84 STMRR27 pKa = 11.84 RR28 pKa = 11.84 RR29 pKa = 11.84 PAMRR33 pKa = 11.84 GTGVRR38 pKa = 11.84 RR39 pKa = 11.84 RR40 pKa = 11.84 IRR42 pKa = 11.84 RR43 pKa = 11.84 ALTAAPMTIRR53 pKa = 11.84 RR54 pKa = 11.84 LLGLGRR60 pKa = 11.84 RR61 pKa = 11.84 RR62 pKa = 11.84 LPRR65 pKa = 11.84 TGSRR69 pKa = 11.84 TTLVAVTSTRR79 pKa = 11.84 RR80 pKa = 11.84 RR81 pKa = 11.84 RR82 pKa = 11.84 RR83 pKa = 11.84 RR84 pKa = 3.19
Molecular weight: 9.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.316
IPC_protein 12.939
Toseland 13.1
ProMoST 13.598
Dawson 13.1
Bjellqvist 13.1
Wikipedia 13.583
Rodwell 12.603
Grimsley 13.144
Solomon 13.598
Lehninger 13.495
Nozaki 13.1
DTASelect 13.1
Thurlkill 13.1
EMBOSS 13.598
Sillero 13.1
Patrickios 12.31
IPC_peptide 13.598
IPC2_peptide 12.588
IPC2.peptide.svr19 9.269
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
34
0
34
13135
46
1270
386.3
43.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.71 ± 0.391
1.979 ± 0.223
5.489 ± 0.256
5.832 ± 0.652
4.537 ± 0.259
5.908 ± 0.313
2.101 ± 0.197
6.007 ± 0.306
4.72 ± 0.459
7.773 ± 0.326
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.099 ± 0.207
5.367 ± 0.487
5.443 ± 0.377
3.662 ± 0.256
5.969 ± 0.526
8.816 ± 0.444
5.961 ± 0.302
6.349 ± 0.393
1.363 ± 0.097
3.913 ± 0.266
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here