Aspergillus wentii DTO 134E9
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12389 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L9RZ02|A0A1L9RZ02_ASPWE HET domain-containing protein OS=Aspergillus wentii DTO 134E9 OX=1073089 GN=ASPWEDRAFT_122800 PE=4 SV=1
MM1 pKa = 7.06 KK2 pKa = 9.69 TFSIALAFLAATGATAISNSEE23 pKa = 4.13 CAKK26 pKa = 9.85 MCISNMNNKK35 pKa = 9.66 ASEE38 pKa = 4.43 LGCKK42 pKa = 9.82 SGDD45 pKa = 3.67 MQCLCNAPDD54 pKa = 3.57 YY55 pKa = 11.34 SNGVRR60 pKa = 11.84 DD61 pKa = 4.58 CTAQACPEE69 pKa = 4.5 DD70 pKa = 4.76 DD71 pKa = 3.45 SAAVVQSALSSCPDD85 pKa = 3.09 GTKK88 pKa = 10.26 AASSASAGSDD98 pKa = 3.2 STATASGSDD107 pKa = 3.6 STSTGTGAAGGASATDD123 pKa = 3.51 ATSTGDD129 pKa = 3.44 STASATGTDD138 pKa = 3.67 ASATGTATGSATGTGASSGASSSGAASSGAASSSGSVSGSASASASITTSVAVTTTNSQGDD199 pKa = 3.97 STTFTTATTEE209 pKa = 3.97 ASSSSEE215 pKa = 3.92 TGGSDD220 pKa = 3.03 SGSGSDD226 pKa = 3.88 SGSATTTSDD235 pKa = 3.17 AAGSTDD241 pKa = 3.68 SEE243 pKa = 4.56 GAAPRR248 pKa = 11.84 LAINGAVGALGLAAFFLL265 pKa = 4.72
Molecular weight: 24.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.705
IPC2_protein 3.681
IPC_protein 3.668
Toseland 3.452
ProMoST 3.872
Dawson 3.681
Bjellqvist 3.846
Wikipedia 3.656
Rodwell 3.503
Grimsley 3.363
Solomon 3.668
Lehninger 3.63
Nozaki 3.808
DTASelect 4.075
Thurlkill 3.516
EMBOSS 3.668
Sillero 3.795
Patrickios 0.896
IPC_peptide 3.656
IPC2_peptide 3.77
IPC2.peptide.svr19 3.742
Protein with the highest isoelectric point:
>tr|A0A1L9RPD9|A0A1L9RPD9_ASPWE SHSP domain-containing protein OS=Aspergillus wentii DTO 134E9 OX=1073089 GN=ASPWEDRAFT_130791 PE=3 SV=1
MM1 pKa = 7.86 PLAKK5 pKa = 10.19 SPSQAAVPKK14 pKa = 10.01 SQIARR19 pKa = 11.84 RR20 pKa = 11.84 ARR22 pKa = 11.84 QMSNNLLPRR31 pKa = 11.84 QKK33 pKa = 10.34 PRR35 pKa = 11.84 LSLLQRR41 pKa = 11.84 PLLALSRR48 pKa = 11.84 HH49 pKa = 6.23 RR50 pKa = 11.84 GRR52 pKa = 11.84 QCMLQMRR59 pKa = 11.84 LPLPAVVLKK68 pKa = 10.73 LPIARR73 pKa = 11.84 PVRR76 pKa = 11.84 LRR78 pKa = 11.84 CRR80 pKa = 11.84 VLLLRR85 pKa = 11.84 HH86 pKa = 5.66 HH87 pKa = 6.25 HH88 pKa = 6.37 HH89 pKa = 6.73 LRR91 pKa = 11.84 ILRR94 pKa = 11.84 LQLLPPPLFLRR105 pKa = 11.84 RR106 pKa = 11.84 RR107 pKa = 11.84 RR108 pKa = 11.84 HH109 pKa = 5.52 LLPLLRR115 pKa = 11.84 CRR117 pKa = 11.84 ARR119 pKa = 11.84 LLCMLPMKK127 pKa = 10.2 LLSLAAVLKK136 pKa = 10.79 LPTAQRR142 pKa = 11.84 VRR144 pKa = 11.84 QQCSRR149 pKa = 11.84 VFLLRR154 pKa = 11.84 RR155 pKa = 11.84 RR156 pKa = 11.84 RR157 pKa = 11.84 RR158 pKa = 11.84 LL159 pKa = 3.19
Molecular weight: 18.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.408
IPC2_protein 10.877
IPC_protein 12.471
Toseland 12.647
ProMoST 13.144
Dawson 12.647
Bjellqvist 12.632
Wikipedia 13.115
Rodwell 12.223
Grimsley 12.676
Solomon 13.144
Lehninger 13.042
Nozaki 12.647
DTASelect 12.632
Thurlkill 12.647
EMBOSS 13.144
Sillero 12.647
Patrickios 11.945
IPC_peptide 13.144
IPC2_peptide 12.135
IPC2.peptide.svr19 9.133
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12389
0
12389
5719169
49
6235
461.6
51.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.083 ± 0.02
1.318 ± 0.009
5.715 ± 0.016
6.096 ± 0.023
3.927 ± 0.014
6.802 ± 0.021
2.445 ± 0.01
5.175 ± 0.015
4.753 ± 0.019
8.993 ± 0.024
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.225 ± 0.008
3.843 ± 0.011
5.92 ± 0.027
4.034 ± 0.016
5.909 ± 0.018
8.416 ± 0.022
5.829 ± 0.011
6.132 ± 0.016
1.502 ± 0.008
2.882 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here