Sulfolobales virus YNP1
Average proteome isoelectric point is 6.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0N9P9W2|A0A0N9P9W2_9VIRU Uncharacterized protein OS=Sulfolobales virus YNP1 OX=1732179 PE=4 SV=1
MM1 pKa = 7.76 KK2 pKa = 10.5 LLLLLAPFLASLLAMGITGGAVLDD26 pKa = 4.08 WSDD29 pKa = 3.29 GSAVVTIQPLSEE41 pKa = 4.11 TVIDD45 pKa = 4.57 GYY47 pKa = 10.52 VVNAFYY53 pKa = 10.86 NGSNQVVLLAEE64 pKa = 5.17 PYY66 pKa = 10.07 YY67 pKa = 10.45 QGQNLQQVTFSLYY80 pKa = 10.49 DD81 pKa = 3.42 YY82 pKa = 7.88 YY83 pKa = 11.0 THH85 pKa = 6.67 QLIGNYY91 pKa = 8.83 TIEE94 pKa = 4.34 DD95 pKa = 4.18 NYY97 pKa = 11.11 TVVTVPSNITVIFIYY112 pKa = 10.49 FDD114 pKa = 3.46 SQQFGPFYY122 pKa = 10.45 VTTNGGNYY130 pKa = 9.74 APPSLEE136 pKa = 3.65 QMLMYY141 pKa = 9.27 VIPLSSVALFGLRR154 pKa = 11.84 AGLRR158 pKa = 11.84 NVGLGLIVSSVFTTAEE174 pKa = 3.98 MVALGVSNPWLYY186 pKa = 10.85 AIPTLEE192 pKa = 3.97 IMFAVILLWHH202 pKa = 6.4 SVQTSGG208 pKa = 5.15
Molecular weight: 22.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.041
IPC2_protein 4.113
IPC_protein 3.986
Toseland 3.808
ProMoST 4.139
Dawson 3.961
Bjellqvist 4.126
Wikipedia 3.884
Rodwell 3.821
Grimsley 3.732
Solomon 3.948
Lehninger 3.897
Nozaki 4.101
DTASelect 4.266
Thurlkill 3.859
EMBOSS 3.897
Sillero 4.113
Patrickios 0.006
IPC_peptide 3.948
IPC2_peptide 4.088
IPC2.peptide.svr19 4.015
Protein with the highest isoelectric point:
>tr|A0A0N9NK95|A0A0N9NK95_9VIRU Uncharacterized protein OS=Sulfolobales virus YNP1 OX=1732179 PE=4 SV=1
MM1 pKa = 7.72 AYY3 pKa = 9.57 KK4 pKa = 10.25 EE5 pKa = 4.1 HH6 pKa = 5.88 THH8 pKa = 6.76 AKK10 pKa = 7.04 LTEE13 pKa = 3.72 WRR15 pKa = 11.84 RR16 pKa = 11.84 EE17 pKa = 3.73 FAEE20 pKa = 4.13 AARR23 pKa = 11.84 MCSAKK28 pKa = 10.65 VKK30 pKa = 10.26 GLPKK34 pKa = 10.39 GEE36 pKa = 4.07 KK37 pKa = 9.38 LRR39 pKa = 11.84 AYY41 pKa = 8.19 RR42 pKa = 11.84 ACMRR46 pKa = 11.84 EE47 pKa = 4.03 TLKK50 pKa = 10.88 KK51 pKa = 10.3 RR52 pKa = 3.37
Molecular weight: 6.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.23
IPC2_protein 9.472
IPC_protein 9.575
Toseland 10.467
ProMoST 10.16
Dawson 10.555
Bjellqvist 10.175
Wikipedia 10.687
Rodwell 11.082
Grimsley 10.599
Solomon 10.613
Lehninger 10.599
Nozaki 10.452
DTASelect 10.16
Thurlkill 10.452
EMBOSS 10.833
Sillero 10.482
Patrickios 10.862
IPC_peptide 10.613
IPC2_peptide 8.946
IPC2.peptide.svr19 8.474
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
71
0
71
11985
46
1101
168.8
19.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.14 ± 0.336
0.609 ± 0.133
4.556 ± 0.333
7.267 ± 0.49
4.214 ± 0.359
5.148 ± 0.286
1.093 ± 0.117
8.185 ± 0.304
7.593 ± 0.524
9.704 ± 0.341
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.128 ± 0.178
5.373 ± 0.346
4.631 ± 0.438
4.097 ± 1.045
4.122 ± 0.312
6.508 ± 0.523
5.29 ± 0.213
7.626 ± 0.408
1.001 ± 0.132
4.948 ± 0.421
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here