Amino acid dipepetide frequency for Stx2-converting phage Stx2a_F723

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.935AlaAla: 8.935 ± 0.832
1.042AlaCys: 1.042 ± 0.314
5.098AlaAsp: 5.098 ± 0.643
7.455AlaGlu: 7.455 ± 0.763
3.618AlaPhe: 3.618 ± 0.602
7.62AlaGly: 7.62 ± 1.132
1.754AlaHis: 1.754 ± 0.348
4.057AlaIle: 4.057 ± 0.496
4.824AlaLys: 4.824 ± 0.545
7.072AlaLeu: 7.072 ± 0.627
3.289AlaMet: 3.289 ± 0.399
2.631AlaAsn: 2.631 ± 0.396
3.07AlaPro: 3.07 ± 0.429
5.043AlaGln: 5.043 ± 0.679
6.14AlaArg: 6.14 ± 0.621
5.427AlaSer: 5.427 ± 0.496
5.591AlaThr: 5.591 ± 0.757
6.304AlaVal: 6.304 ± 0.619
1.754AlaTrp: 1.754 ± 0.267
2.522AlaTyr: 2.522 ± 0.333
0.0AlaXaa: 0.0 ± 0.0
Cys
1.37CysAla: 1.37 ± 0.321
0.493CysCys: 0.493 ± 0.172
0.439CysAsp: 0.439 ± 0.173
0.767CysGlu: 0.767 ± 0.245
0.439CysPhe: 0.439 ± 0.172
0.987CysGly: 0.987 ± 0.286
0.274CysHis: 0.274 ± 0.125
0.658CysIle: 0.658 ± 0.217
0.603CysLys: 0.603 ± 0.189
0.767CysLeu: 0.767 ± 0.274
0.164CysMet: 0.164 ± 0.103
0.274CysAsn: 0.274 ± 0.126
0.439CysPro: 0.439 ± 0.188
0.384CysGln: 0.384 ± 0.145
1.206CysArg: 1.206 ± 0.351
0.877CysSer: 0.877 ± 0.267
0.658CysThr: 0.658 ± 0.212
0.877CysVal: 0.877 ± 0.273
0.11CysTrp: 0.11 ± 0.08
0.384CysTyr: 0.384 ± 0.142
0.0CysXaa: 0.0 ± 0.0
Asp
5.646AspAla: 5.646 ± 0.589
0.822AspCys: 0.822 ± 0.262
3.728AspAsp: 3.728 ± 0.398
4.331AspGlu: 4.331 ± 0.441
1.48AspPhe: 1.48 ± 0.289
4.55AspGly: 4.55 ± 0.518
0.987AspHis: 0.987 ± 0.264
3.618AspIle: 3.618 ± 0.379
3.837AspLys: 3.837 ± 0.453
4.057AspLeu: 4.057 ± 0.543
1.973AspMet: 1.973 ± 0.311
2.686AspAsn: 2.686 ± 0.381
2.357AspPro: 2.357 ± 0.357
1.48AspGln: 1.48 ± 0.293
3.234AspArg: 3.234 ± 0.358
3.07AspSer: 3.07 ± 0.434
2.796AspThr: 2.796 ± 0.457
4.111AspVal: 4.111 ± 0.453
1.261AspTrp: 1.261 ± 0.36
1.59AspTyr: 1.59 ± 0.346
0.0AspXaa: 0.0 ± 0.0
Glu
6.578GluAla: 6.578 ± 0.531
0.767GluCys: 0.767 ± 0.222
1.973GluAsp: 1.973 ± 0.322
3.563GluGlu: 3.563 ± 0.397
2.631GluPhe: 2.631 ± 0.387
3.947GluGly: 3.947 ± 0.554
1.316GluHis: 1.316 ± 0.244
3.728GluIle: 3.728 ± 0.473
4.44GluLys: 4.44 ± 0.477
5.975GluLeu: 5.975 ± 0.655
2.193GluMet: 2.193 ± 0.32
3.179GluAsn: 3.179 ± 0.47
1.864GluPro: 1.864 ± 0.447
3.892GluGln: 3.892 ± 0.552
5.92GluArg: 5.92 ± 0.614
3.892GluSer: 3.892 ± 0.493
3.947GluThr: 3.947 ± 0.744
4.276GluVal: 4.276 ± 0.605
1.316GluTrp: 1.316 ± 0.232
2.193GluTyr: 2.193 ± 0.344
0.0GluXaa: 0.0 ± 0.0
Phe
3.125PheAla: 3.125 ± 0.396
0.439PheCys: 0.439 ± 0.185
1.754PheAsp: 1.754 ± 0.269
1.425PheGlu: 1.425 ± 0.331
0.822PhePhe: 0.822 ± 0.244
2.412PheGly: 2.412 ± 0.282
0.658PheHis: 0.658 ± 0.161
1.809PheIle: 1.809 ± 0.294
1.699PheLys: 1.699 ± 0.289
2.028PheLeu: 2.028 ± 0.288
0.987PheMet: 0.987 ± 0.226
1.59PheAsn: 1.59 ± 0.32
1.206PhePro: 1.206 ± 0.292
0.713PheGln: 0.713 ± 0.197
2.412PheArg: 2.412 ± 0.34
2.796PheSer: 2.796 ± 0.473
2.467PheThr: 2.467 ± 0.362
2.412PheVal: 2.412 ± 0.363
0.822PheTrp: 0.822 ± 0.229
1.151PheTyr: 1.151 ± 0.215
0.0PheXaa: 0.0 ± 0.0
Gly
5.646GlyAla: 5.646 ± 1.015
1.042GlyCys: 1.042 ± 0.378
4.714GlyAsp: 4.714 ± 0.8
5.92GlyGlu: 5.92 ± 1.525
2.796GlyPhe: 2.796 ± 0.406
4.55GlyGly: 4.55 ± 0.69
1.151GlyHis: 1.151 ± 0.276
4.44GlyIle: 4.44 ± 0.687
4.879GlyLys: 4.879 ± 1.021
5.208GlyLeu: 5.208 ± 0.457
2.193GlyMet: 2.193 ± 0.324
2.796GlyAsn: 2.796 ± 0.379
3.892GlyPro: 3.892 ± 2.333
2.522GlyGln: 2.522 ± 0.388
4.057GlyArg: 4.057 ± 0.575
3.892GlySer: 3.892 ± 0.518
3.179GlyThr: 3.179 ± 0.45
5.208GlyVal: 5.208 ± 0.491
1.206GlyTrp: 1.206 ± 0.266
2.467GlyTyr: 2.467 ± 0.356
0.0GlyXaa: 0.0 ± 0.0
His
1.535HisAla: 1.535 ± 0.273
0.219HisCys: 0.219 ± 0.104
1.151HisAsp: 1.151 ± 0.278
0.767HisGlu: 0.767 ± 0.225
0.713HisPhe: 0.713 ± 0.275
1.59HisGly: 1.59 ± 0.303
0.439HisHis: 0.439 ± 0.155
0.767HisIle: 0.767 ± 0.232
1.042HisLys: 1.042 ± 0.282
1.535HisLeu: 1.535 ± 0.397
0.219HisMet: 0.219 ± 0.114
0.987HisAsn: 0.987 ± 0.201
0.932HisPro: 0.932 ± 0.283
0.658HisGln: 0.658 ± 0.204
1.096HisArg: 1.096 ± 0.272
0.932HisSer: 0.932 ± 0.206
0.932HisThr: 0.932 ± 0.229
0.822HisVal: 0.822 ± 0.211
0.439HisTrp: 0.439 ± 0.21
0.822HisTyr: 0.822 ± 0.2
0.0HisXaa: 0.0 ± 0.0
Ile
4.66IleAla: 4.66 ± 0.487
1.042IleCys: 1.042 ± 0.286
3.728IleAsp: 3.728 ± 0.483
3.618IleGlu: 3.618 ± 0.476
1.096IlePhe: 1.096 ± 0.235
2.631IleGly: 2.631 ± 0.484
0.987IleHis: 0.987 ± 0.266
2.412IleIle: 2.412 ± 0.386
3.179IleLys: 3.179 ± 0.416
3.179IleLeu: 3.179 ± 0.509
1.042IleMet: 1.042 ± 0.26
3.344IleAsn: 3.344 ± 0.409
2.522IlePro: 2.522 ± 0.427
2.083IleGln: 2.083 ± 0.289
4.331IleArg: 4.331 ± 0.497
4.44IleSer: 4.44 ± 0.563
3.728IleThr: 3.728 ± 0.556
2.357IleVal: 2.357 ± 0.471
0.603IleTrp: 0.603 ± 0.201
1.699IleTyr: 1.699 ± 0.376
0.0IleXaa: 0.0 ± 0.0
Lys
6.194LysAla: 6.194 ± 0.623
0.603LysCys: 0.603 ± 0.205
3.015LysAsp: 3.015 ± 0.449
4.331LysGlu: 4.331 ± 0.491
1.206LysPhe: 1.206 ± 0.232
5.646LysGly: 5.646 ± 1.162
0.822LysHis: 0.822 ± 0.174
3.454LysIle: 3.454 ± 0.425
3.947LysLys: 3.947 ± 0.611
5.153LysLeu: 5.153 ± 0.536
1.919LysMet: 1.919 ± 0.308
3.673LysAsn: 3.673 ± 0.389
2.851LysPro: 2.851 ± 0.492
2.796LysGln: 2.796 ± 0.494
3.07LysArg: 3.07 ± 0.419
3.125LysSer: 3.125 ± 0.414
3.07LysThr: 3.07 ± 0.375
3.015LysVal: 3.015 ± 0.466
0.767LysTrp: 0.767 ± 0.234
1.919LysTyr: 1.919 ± 0.347
0.0LysXaa: 0.0 ± 0.0
Leu
8.716LeuAla: 8.716 ± 0.744
0.877LeuCys: 0.877 ± 0.281
3.947LeuAsp: 3.947 ± 0.499
4.002LeuGlu: 4.002 ± 0.518
2.686LeuPhe: 2.686 ± 0.459
4.166LeuGly: 4.166 ± 0.441
1.37LeuHis: 1.37 ± 0.247
3.728LeuIle: 3.728 ± 0.514
4.824LeuLys: 4.824 ± 0.562
5.975LeuLeu: 5.975 ± 0.608
2.193LeuMet: 2.193 ± 0.384
4.385LeuAsn: 4.385 ± 0.537
3.673LeuPro: 3.673 ± 0.449
3.015LeuGln: 3.015 ± 0.55
5.043LeuArg: 5.043 ± 0.522
5.482LeuSer: 5.482 ± 0.635
5.043LeuThr: 5.043 ± 0.443
4.495LeuVal: 4.495 ± 0.476
0.767LeuTrp: 0.767 ± 0.254
2.576LeuTyr: 2.576 ± 0.448
0.0LeuXaa: 0.0 ± 0.0
Met
3.125MetAla: 3.125 ± 0.363
0.11MetCys: 0.11 ± 0.083
1.37MetAsp: 1.37 ± 0.262
1.535MetGlu: 1.535 ± 0.272
0.658MetPhe: 0.658 ± 0.183
1.48MetGly: 1.48 ± 0.321
0.384MetHis: 0.384 ± 0.126
0.877MetIle: 0.877 ± 0.166
2.248MetLys: 2.248 ± 0.35
1.973MetLeu: 1.973 ± 0.32
0.767MetMet: 0.767 ± 0.193
1.48MetAsn: 1.48 ± 0.328
1.535MetPro: 1.535 ± 0.292
1.37MetGln: 1.37 ± 0.254
1.699MetArg: 1.699 ± 0.319
2.028MetSer: 2.028 ± 0.318
2.796MetThr: 2.796 ± 0.316
1.535MetVal: 1.535 ± 0.308
0.274MetTrp: 0.274 ± 0.125
0.548MetTyr: 0.548 ± 0.18
0.0MetXaa: 0.0 ± 0.0
Asn
4.385AsnAla: 4.385 ± 0.686
0.439AsnCys: 0.439 ± 0.178
2.302AsnAsp: 2.302 ± 0.463
3.07AsnGlu: 3.07 ± 0.477
1.535AsnPhe: 1.535 ± 0.351
3.947AsnGly: 3.947 ± 0.39
1.37AsnHis: 1.37 ± 0.303
3.07AsnIle: 3.07 ± 0.396
2.522AsnLys: 2.522 ± 0.373
3.454AsnLeu: 3.454 ± 0.443
1.425AsnMet: 1.425 ± 0.283
1.754AsnAsn: 1.754 ± 0.356
1.535AsnPro: 1.535 ± 0.297
2.138AsnGln: 2.138 ± 0.369
2.741AsnArg: 2.741 ± 0.481
2.686AsnSer: 2.686 ± 0.438
2.412AsnThr: 2.412 ± 0.343
2.083AsnVal: 2.083 ± 0.476
0.548AsnTrp: 0.548 ± 0.166
1.096AsnTyr: 1.096 ± 0.247
0.0AsnXaa: 0.0 ± 0.0
Pro
3.234ProAla: 3.234 ± 0.62
0.329ProCys: 0.329 ± 0.124
4.111ProAsp: 4.111 ± 0.498
5.153ProGlu: 5.153 ± 0.719
1.425ProPhe: 1.425 ± 0.248
3.07ProGly: 3.07 ± 0.587
0.384ProHis: 0.384 ± 0.154
1.206ProIle: 1.206 ± 0.307
2.796ProLys: 2.796 ± 0.781
2.522ProLeu: 2.522 ± 0.354
0.767ProMet: 0.767 ± 0.187
1.206ProAsn: 1.206 ± 0.277
1.425ProPro: 1.425 ± 0.311
2.028ProGln: 2.028 ± 0.556
2.083ProArg: 2.083 ± 0.397
2.467ProSer: 2.467 ± 0.359
1.699ProThr: 1.699 ± 0.303
4.66ProVal: 4.66 ± 0.491
0.658ProTrp: 0.658 ± 0.195
1.535ProTyr: 1.535 ± 0.343
0.0ProXaa: 0.0 ± 0.0
Gln
4.385GlnAla: 4.385 ± 0.549
0.877GlnCys: 0.877 ± 0.264
2.302GlnAsp: 2.302 ± 0.372
2.741GlnGlu: 2.741 ± 0.404
1.699GlnPhe: 1.699 ± 0.326
3.289GlnGly: 3.289 ± 0.728
0.822GlnHis: 0.822 ± 0.189
2.028GlnIle: 2.028 ± 0.354
3.015GlnLys: 3.015 ± 0.465
3.563GlnLeu: 3.563 ± 0.477
0.987GlnMet: 0.987 ± 0.295
1.754GlnAsn: 1.754 ± 0.313
1.919GlnPro: 1.919 ± 0.409
3.563GlnGln: 3.563 ± 0.749
3.015GlnArg: 3.015 ± 0.567
2.248GlnSer: 2.248 ± 0.312
1.919GlnThr: 1.919 ± 0.382
2.522GlnVal: 2.522 ± 0.508
0.822GlnTrp: 0.822 ± 0.208
1.645GlnTyr: 1.645 ± 0.302
0.0GlnXaa: 0.0 ± 0.0
Arg
4.714ArgAla: 4.714 ± 0.447
0.384ArgCys: 0.384 ± 0.15
4.221ArgAsp: 4.221 ± 0.648
5.427ArgGlu: 5.427 ± 0.702
2.248ArgPhe: 2.248 ± 0.355
4.44ArgGly: 4.44 ± 0.934
1.59ArgHis: 1.59 ± 0.252
3.892ArgIle: 3.892 ± 0.613
4.331ArgLys: 4.331 ± 0.469
5.208ArgLeu: 5.208 ± 0.36
1.754ArgMet: 1.754 ± 0.302
3.07ArgAsn: 3.07 ± 0.436
2.248ArgPro: 2.248 ± 0.416
3.454ArgGln: 3.454 ± 0.427
5.646ArgArg: 5.646 ± 0.69
3.947ArgSer: 3.947 ± 0.438
3.289ArgThr: 3.289 ± 0.634
4.057ArgVal: 4.057 ± 0.487
1.316ArgTrp: 1.316 ± 0.256
2.138ArgTyr: 2.138 ± 0.446
0.0ArgXaa: 0.0 ± 0.0
Ser
5.756SerAla: 5.756 ± 0.595
0.548SerCys: 0.548 ± 0.2
3.728SerAsp: 3.728 ± 0.439
4.385SerGlu: 4.385 ± 0.46
1.809SerPhe: 1.809 ± 0.26
5.591SerGly: 5.591 ± 0.574
0.658SerHis: 0.658 ± 0.167
3.015SerIle: 3.015 ± 0.395
3.179SerLys: 3.179 ± 0.486
5.866SerLeu: 5.866 ± 0.662
1.535SerMet: 1.535 ± 0.408
2.357SerAsn: 2.357 ± 0.337
3.125SerPro: 3.125 ± 0.454
2.96SerGln: 2.96 ± 0.439
3.892SerArg: 3.892 ± 0.466
2.96SerSer: 2.96 ± 0.543
3.782SerThr: 3.782 ± 0.405
3.728SerVal: 3.728 ± 0.477
0.877SerTrp: 0.877 ± 0.21
1.645SerTyr: 1.645 ± 0.355
0.0SerXaa: 0.0 ± 0.0
Thr
5.263ThrAla: 5.263 ± 0.445
0.603ThrCys: 0.603 ± 0.197
3.728ThrAsp: 3.728 ± 0.453
3.618ThrGlu: 3.618 ± 0.399
1.864ThrPhe: 1.864 ± 0.383
5.317ThrGly: 5.317 ± 0.852
0.987ThrHis: 0.987 ± 0.221
3.673ThrIle: 3.673 ± 0.406
2.905ThrLys: 2.905 ± 0.424
4.988ThrLeu: 4.988 ± 0.519
0.877ThrMet: 0.877 ± 0.193
2.193ThrAsn: 2.193 ± 0.322
3.454ThrPro: 3.454 ± 0.36
1.59ThrGln: 1.59 ± 0.323
2.905ThrArg: 2.905 ± 0.443
3.892ThrSer: 3.892 ± 0.507
3.125ThrThr: 3.125 ± 0.471
3.892ThrVal: 3.892 ± 0.501
0.767ThrTrp: 0.767 ± 0.183
1.206ThrTyr: 1.206 ± 0.296
0.0ThrXaa: 0.0 ± 0.0
Val
6.249ValAla: 6.249 ± 0.584
0.932ValCys: 0.932 ± 0.239
3.728ValAsp: 3.728 ± 0.395
3.508ValGlu: 3.508 ± 0.369
2.028ValPhe: 2.028 ± 0.272
3.289ValGly: 3.289 ± 0.485
0.548ValHis: 0.548 ± 0.165
3.618ValIle: 3.618 ± 0.528
3.837ValLys: 3.837 ± 0.459
5.372ValLeu: 5.372 ± 0.602
1.809ValMet: 1.809 ± 0.344
3.07ValAsn: 3.07 ± 0.369
2.741ValPro: 2.741 ± 0.355
2.357ValGln: 2.357 ± 0.37
4.714ValArg: 4.714 ± 1.013
4.55ValSer: 4.55 ± 0.545
3.947ValThr: 3.947 ± 0.424
4.221ValVal: 4.221 ± 0.474
1.042ValTrp: 1.042 ± 0.218
2.193ValTyr: 2.193 ± 0.284
0.0ValXaa: 0.0 ± 0.0
Trp
0.987TrpAla: 0.987 ± 0.231
0.274TrpCys: 0.274 ± 0.124
0.548TrpAsp: 0.548 ± 0.215
0.713TrpGlu: 0.713 ± 0.184
0.713TrpPhe: 0.713 ± 0.185
0.767TrpGly: 0.767 ± 0.237
0.329TrpHis: 0.329 ± 0.125
0.877TrpIle: 0.877 ± 0.209
1.206TrpLys: 1.206 ± 0.233
1.699TrpLeu: 1.699 ± 0.372
0.713TrpMet: 0.713 ± 0.201
0.767TrpAsn: 0.767 ± 0.222
0.603TrpPro: 0.603 ± 0.213
1.316TrpGln: 1.316 ± 0.22
1.59TrpArg: 1.59 ± 0.295
0.932TrpSer: 0.932 ± 0.217
0.603TrpThr: 0.603 ± 0.188
1.206TrpVal: 1.206 ± 0.257
0.493TrpTrp: 0.493 ± 0.172
0.329TrpTyr: 0.329 ± 0.124
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.741TyrAla: 2.741 ± 0.391
0.329TyrCys: 0.329 ± 0.134
2.028TyrAsp: 2.028 ± 0.315
1.261TyrGlu: 1.261 ± 0.289
1.316TyrPhe: 1.316 ± 0.229
2.467TyrGly: 2.467 ± 0.424
0.767TyrHis: 0.767 ± 0.234
1.754TyrIle: 1.754 ± 0.287
1.261TyrLys: 1.261 ± 0.265
1.37TyrLeu: 1.37 ± 0.276
0.932TyrMet: 0.932 ± 0.229
1.316TyrAsn: 1.316 ± 0.263
1.37TyrPro: 1.37 ± 0.237
1.754TyrGln: 1.754 ± 0.288
2.576TyrArg: 2.576 ± 0.426
1.754TyrSer: 1.754 ± 0.275
1.864TyrThr: 1.864 ± 0.457
1.973TyrVal: 1.973 ± 0.319
0.822TyrTrp: 0.822 ± 0.253
1.206TyrTyr: 1.206 ± 0.225
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 86 proteins (18243 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski