Maize streak virus
Average proteome isoelectric point is 7.05
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B6CZV2|B6CZV2_9GEMI Replication-associated protein OS=Maize streak virus OX=10821 PE=3 SV=1
MM1 pKa = 7.36 TSSSSNRR8 pKa = 11.84 QFLHH12 pKa = 6.69 RR13 pKa = 11.84 NVNTFLTYY21 pKa = 8.18 PHH23 pKa = 7.01 CPEE26 pKa = 3.84 NPEE29 pKa = 4.12 IVSQKK34 pKa = 9.79 LWEE37 pKa = 4.12 LVGRR41 pKa = 11.84 WKK43 pKa = 10.25 PLYY46 pKa = 9.81 IICARR51 pKa = 11.84 EE52 pKa = 3.67 AHH54 pKa = 6.6 EE55 pKa = 5.52 DD56 pKa = 3.97 GNLHH60 pKa = 6.05 LHH62 pKa = 6.22 VLIQTDD68 pKa = 3.77 KK69 pKa = 10.81 PVRR72 pKa = 11.84 TSDD75 pKa = 3.81 SRR77 pKa = 11.84 FFDD80 pKa = 2.91 IDD82 pKa = 3.48 GFHH85 pKa = 7.27 PNIQSAMSVNKK96 pKa = 9.81 VRR98 pKa = 11.84 DD99 pKa = 3.94 YY100 pKa = 11.14 ILKK103 pKa = 10.2 EE104 pKa = 3.57 PLALFEE110 pKa = 5.16 RR111 pKa = 11.84 GTFIPRR117 pKa = 11.84 KK118 pKa = 9.66 KK119 pKa = 10.36 SVLGNSSKK127 pKa = 11.16 GNSEE131 pKa = 4.12 KK132 pKa = 10.7 KK133 pKa = 9.55 PSKK136 pKa = 10.67 DD137 pKa = 3.89 EE138 pKa = 3.83 IMQDD142 pKa = 3.59 IISHH146 pKa = 5.63 ATSKK150 pKa = 10.82 RR151 pKa = 11.84 EE152 pKa = 3.77 YY153 pKa = 10.24 LSMVRR158 pKa = 11.84 KK159 pKa = 9.97 SFPYY163 pKa = 10.17 DD164 pKa = 2.76 WATKK168 pKa = 9.61 LQYY171 pKa = 10.4 FEE173 pKa = 4.89 YY174 pKa = 10.06 SASKK178 pKa = 10.37 LFPDD182 pKa = 3.43 IQEE185 pKa = 4.21 EE186 pKa = 4.74 FINPHH191 pKa = 6.03 PTSEE195 pKa = 4.43 PDD197 pKa = 3.53 LLCNEE202 pKa = 5.12 SIKK205 pKa = 11.07 DD206 pKa = 3.33 WLQPNIYY213 pKa = 9.41 QVSPEE218 pKa = 4.47 AYY220 pKa = 8.91 MLLQPTCYY228 pKa = 10.31 SVDD231 pKa = 3.65 DD232 pKa = 5.6 AISDD236 pKa = 4.18 LQWLDD241 pKa = 3.79 DD242 pKa = 4.03 VSSKK246 pKa = 11.14 QIMDD250 pKa = 3.34 QGSRR254 pKa = 11.84 ASTSSVQQGQGNLLGPEE271 pKa = 3.97 AA272 pKa = 4.96
Molecular weight: 31.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.56
IPC2_protein 5.525
IPC_protein 5.537
Toseland 5.893
ProMoST 5.842
Dawson 5.741
Bjellqvist 5.766
Wikipedia 5.728
Rodwell 5.715
Grimsley 6.033
Solomon 5.741
Lehninger 5.728
Nozaki 5.982
DTASelect 6.173
Thurlkill 6.135
EMBOSS 6.097
Sillero 6.071
Patrickios 4.101
IPC_peptide 5.753
IPC2_peptide 6.071
IPC2.peptide.svr19 6.089
Protein with the highest isoelectric point:
>tr|B6CZV0|B6CZV0_9GEMI Movement protein OS=Maize streak virus OX=10821 PE=3 SV=1
MM1 pKa = 6.95 STSKK5 pKa = 10.63 RR6 pKa = 11.84 KK7 pKa = 9.69 RR8 pKa = 11.84 ADD10 pKa = 3.22 EE11 pKa = 4.25 AQWNQRR17 pKa = 11.84 STKK20 pKa = 9.94 KK21 pKa = 10.25 KK22 pKa = 10.28 ASAPKK27 pKa = 9.94 AKK29 pKa = 10.2 KK30 pKa = 10.35 AGGKK34 pKa = 8.85 GEE36 pKa = 4.18 KK37 pKa = 9.62 PSLQIQTLLHH47 pKa = 6.55 SGDD50 pKa = 3.49 TMITVPSGGVCDD62 pKa = 6.17 LINTYY67 pKa = 11.02 ARR69 pKa = 11.84 GSDD72 pKa = 3.04 EE73 pKa = 4.39 GNRR76 pKa = 11.84 HH77 pKa = 4.54 TSEE80 pKa = 3.79 TLTYY84 pKa = 10.21 KK85 pKa = 10.8 VGVDD89 pKa = 3.04 YY90 pKa = 11.43 HH91 pKa = 6.46 FVADD95 pKa = 4.09 AASCKK100 pKa = 9.91 YY101 pKa = 10.2 SNRR104 pKa = 11.84 GTGVMWLVYY113 pKa = 9.39 DD114 pKa = 4.05 TTPGGNAPTTKK125 pKa = 10.51 DD126 pKa = 2.52 IFAYY130 pKa = 10.59 SDD132 pKa = 3.75 ALKK135 pKa = 10.5 AWPTTWKK142 pKa = 10.31 VSRR145 pKa = 11.84 EE146 pKa = 3.85 LCHH149 pKa = 6.49 RR150 pKa = 11.84 FVVKK154 pKa = 10.41 RR155 pKa = 11.84 RR156 pKa = 11.84 WLFTMEE162 pKa = 3.72 TDD164 pKa = 3.84 GRR166 pKa = 11.84 IGSDD170 pKa = 3.46 TPPSNASWPPCKK182 pKa = 10.18 RR183 pKa = 11.84 NVDD186 pKa = 4.1 FHH188 pKa = 7.43 KK189 pKa = 9.79 FTSGLGVRR197 pKa = 11.84 TQWKK201 pKa = 8.76 NVTDD205 pKa = 4.02 GGVGAIQRR213 pKa = 11.84 GALYY217 pKa = 10.5 LVIAPGNGITFTAHH231 pKa = 5.19 GQTRR235 pKa = 11.84 LYY237 pKa = 9.73 FKK239 pKa = 10.92 SVGNQQ244 pKa = 2.8
Molecular weight: 26.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.098
IPC2_protein 9.224
IPC_protein 9.165
Toseland 9.999
ProMoST 9.633
Dawson 10.175
Bjellqvist 9.838
Wikipedia 10.321
Rodwell 10.687
Grimsley 10.233
Solomon 10.204
Lehninger 10.175
Nozaki 10.028
DTASelect 9.823
Thurlkill 10.043
EMBOSS 10.394
Sillero 10.101
Patrickios 10.292
IPC_peptide 10.204
IPC2_peptide 8.478
IPC2.peptide.svr19 8.157
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
617
101
272
205.7
23.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.159 ± 1.294
1.459 ± 0.139
5.511 ± 0.833
4.538 ± 1.189
3.566 ± 0.298
6.969 ± 2.09
2.593 ± 0.234
5.186 ± 1.031
6.159 ± 1.541
7.942 ± 1.516
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.783 ± 0.29
4.052 ± 0.626
6.483 ± 1.37
4.214 ± 1.051
5.511 ± 0.688
8.914 ± 1.542
6.969 ± 1.945
6.159 ± 0.971
2.431 ± 0.363
3.404 ± 0.178
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here