Golovinomyces cichoracearum

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Erysiphales; Erysiphaceae; Golovinomyces

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6757 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A420I8Q3|A0A420I8Q3_9PEZI Uncharacterized protein OS=Golovinomyces cichoracearum OX=62708 GN=GcM3_112013b PE=4 SV=1
MM1 pKa = 7.66EE2 pKa = 4.58YY3 pKa = 10.35EE4 pKa = 4.36GDD6 pKa = 3.89SEE8 pKa = 4.82EE9 pKa = 4.41EE10 pKa = 3.85MTAVFQSILLDD21 pKa = 3.62EE22 pKa = 5.29DD23 pKa = 4.58EE24 pKa = 4.52NDD26 pKa = 3.69GYY28 pKa = 11.45YY29 pKa = 10.71EE30 pKa = 4.89NEE32 pKa = 3.92NCKK35 pKa = 11.17DD36 pKa = 3.2NDD38 pKa = 3.37NYY40 pKa = 11.32NFNEE44 pKa = 4.25RR45 pKa = 11.84ANSTSYY51 pKa = 8.8ITNFEE56 pKa = 4.23SLPVGSIFHH65 pKa = 6.4TKK67 pKa = 10.34FSPSPIDD74 pKa = 3.5YY75 pKa = 10.34RR76 pKa = 11.84ITKK79 pKa = 9.59

Molecular weight: 9.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A420GQ19|A0A420GQ19_9PEZI Histone-glutamine methyltransferase OS=Golovinomyces cichoracearum OX=62708 GN=GcM3_224030 PE=3 SV=1
MM1 pKa = 7.41SHH3 pKa = 6.25EE4 pKa = 4.71SVWNSRR10 pKa = 11.84PRR12 pKa = 11.84TYY14 pKa = 11.25GKK16 pKa = 10.48GSRR19 pKa = 11.84SCRR22 pKa = 11.84ICTHH26 pKa = 6.26RR27 pKa = 11.84AGVIRR32 pKa = 11.84KK33 pKa = 9.03YY34 pKa = 11.06GLDD37 pKa = 2.84ICRR40 pKa = 11.84QCFRR44 pKa = 11.84EE45 pKa = 4.08KK46 pKa = 10.75ASDD49 pKa = 3.05IGFVKK54 pKa = 10.64VSSLKK59 pKa = 9.45NSIVRR64 pKa = 11.84EE65 pKa = 4.15HH66 pKa = 6.23QLTRR70 pKa = 11.84PP71 pKa = 3.6

Molecular weight: 8.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6757

0

6757

3060206

31

7682

452.9

50.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.396 ± 0.026

1.317 ± 0.012

5.48 ± 0.022

6.612 ± 0.028

3.898 ± 0.019

5.517 ± 0.024

2.279 ± 0.011

6.437 ± 0.021

6.379 ± 0.029

9.164 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.028 ± 0.011

5.083 ± 0.017

5.023 ± 0.025

4.067 ± 0.02

5.751 ± 0.021

9.434 ± 0.038

5.783 ± 0.015

5.334 ± 0.017

1.192 ± 0.01

2.825 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski