Virus sp. ctgoX21
Average proteome isoelectric point is 7.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5Q2WBD0|A0A5Q2WBD0_9VIRU Rep protein OS=Virus sp. ctgoX21 OX=2656731 PE=3 SV=1
MM1 pKa = 7.97 SDD3 pKa = 4.47 LLTNLPTTVDD13 pKa = 3.51 DD14 pKa = 4.5 KK15 pKa = 11.57 FRR17 pKa = 11.84 LQAQRR22 pKa = 11.84 LMLTYY27 pKa = 8.86 KK28 pKa = 9.19 THH30 pKa = 6.74 LDD32 pKa = 3.21 KK33 pKa = 11.78 DD34 pKa = 3.91 EE35 pKa = 4.74 FSEE38 pKa = 4.18 FLSRR42 pKa = 11.84 IKK44 pKa = 10.33 PPKK47 pKa = 7.99 TLYY50 pKa = 9.71 IAHH53 pKa = 7.08 EE54 pKa = 4.7 NGDD57 pKa = 4.45 DD58 pKa = 4.81 DD59 pKa = 6.17 PITPYY64 pKa = 10.8 EE65 pKa = 4.17 HH66 pKa = 5.43 THH68 pKa = 4.58 VVIDD72 pKa = 4.56 FGTAFNNRR80 pKa = 11.84 NCRR83 pKa = 11.84 VFDD86 pKa = 3.85 FEE88 pKa = 5.54 GIHH91 pKa = 5.27 PHH93 pKa = 6.36 ISKK96 pKa = 9.78 IQNKK100 pKa = 9.24 FDD102 pKa = 3.32 WKK104 pKa = 10.24 RR105 pKa = 11.84 ACTYY109 pKa = 7.37 VTKK112 pKa = 10.66 EE113 pKa = 3.91 DD114 pKa = 3.81 TTVEE118 pKa = 3.9 LAEE121 pKa = 4.35 EE122 pKa = 4.4 DD123 pKa = 3.76 SFGVKK128 pKa = 9.94 KK129 pKa = 10.87 NSVLDD134 pKa = 3.14 IWRR137 pKa = 11.84 SRR139 pKa = 11.84 NISEE143 pKa = 4.36 ALEE146 pKa = 4.44 GMSSLRR152 pKa = 11.84 DD153 pKa = 3.82 ALPTIAIFNHH163 pKa = 6.24 KK164 pKa = 9.71 PIEE167 pKa = 4.27 LPQPEE172 pKa = 4.69 LEE174 pKa = 4.99 EE175 pKa = 4.2 SQFWYY180 pKa = 8.52 WQEE183 pKa = 3.8 SCRR186 pKa = 11.84 DD187 pKa = 3.89 LIFTQPNNRR196 pKa = 11.84 SVIWVYY202 pKa = 11.5 DD203 pKa = 3.76 EE204 pKa = 4.21 VGGAGKK210 pKa = 8.21 TRR212 pKa = 11.84 FAEE215 pKa = 4.76 WACLTYY221 pKa = 10.25 PEE223 pKa = 4.97 KK224 pKa = 10.68 CTMFNQIGKK233 pKa = 9.97 VSDD236 pKa = 3.36 FAMNIRR242 pKa = 11.84 SEE244 pKa = 4.12 QLAGWRR250 pKa = 11.84 GDD252 pKa = 3.33 TCFINLSRR260 pKa = 11.84 DD261 pKa = 3.63 YY262 pKa = 11.14 SDD264 pKa = 4.64 KK265 pKa = 11.29 DD266 pKa = 3.45 HH267 pKa = 7.37 IYY269 pKa = 10.4 QALEE273 pKa = 3.89 MIKK276 pKa = 10.57 DD277 pKa = 3.73 RR278 pKa = 11.84 FVTCTKK284 pKa = 9.65 YY285 pKa = 9.46 TGGKK289 pKa = 7.99 IWLPTMHH296 pKa = 6.88 VIVLANFPPMKK307 pKa = 10.25 HH308 pKa = 6.39 KK309 pKa = 10.56 LSKK312 pKa = 10.64 DD313 pKa = 2.5 RR314 pKa = 11.84 WEE316 pKa = 4.45 IYY318 pKa = 10.41 EE319 pKa = 4.25 IDD321 pKa = 3.56 PTEE324 pKa = 3.95 WSWIEE329 pKa = 3.86 RR330 pKa = 11.84 SSGG333 pKa = 3.25
Molecular weight: 38.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.36
IPC2_protein 5.359
IPC_protein 5.347
Toseland 5.525
ProMoST 5.588
Dawson 5.461
Bjellqvist 5.537
Wikipedia 5.397
Rodwell 5.41
Grimsley 5.563
Solomon 5.461
Lehninger 5.436
Nozaki 5.652
DTASelect 5.83
Thurlkill 5.664
EMBOSS 5.626
Sillero 5.741
Patrickios 4.113
IPC_peptide 5.474
IPC2_peptide 5.741
IPC2.peptide.svr19 5.738
Protein with the highest isoelectric point:
>tr|A0A5Q2WBD0|A0A5Q2WBD0_9VIRU Rep protein OS=Virus sp. ctgoX21 OX=2656731 PE=3 SV=1
MM1 pKa = 6.85 YY2 pKa = 9.11 PRR4 pKa = 11.84 RR5 pKa = 11.84 KK6 pKa = 9.97 KK7 pKa = 8.98 YY8 pKa = 8.15 TPKK11 pKa = 9.87 PFVRR15 pKa = 11.84 RR16 pKa = 11.84 SQKK19 pKa = 9.9 PRR21 pKa = 11.84 TAPRR25 pKa = 11.84 RR26 pKa = 11.84 KK27 pKa = 8.08 YY28 pKa = 8.92 TSKK31 pKa = 10.9 KK32 pKa = 7.87 VIKK35 pKa = 9.6 RR36 pKa = 11.84 VVRR39 pKa = 11.84 AVVSRR44 pKa = 11.84 QAEE47 pKa = 4.43 KK48 pKa = 10.82 KK49 pKa = 9.04 SVVYY53 pKa = 10.69 SSTLSLGTLQPTTSSLVGNYY73 pKa = 7.28 MVITPSQSSFGYY85 pKa = 9.0 TIAKK89 pKa = 8.2 GTDD92 pKa = 2.93 DD93 pKa = 3.61 NQRR96 pKa = 11.84 IGNQINIKK104 pKa = 6.41 TLRR107 pKa = 11.84 HH108 pKa = 4.98 TFVCIVNPYY117 pKa = 10.03 NATTNTQQRR126 pKa = 11.84 PCFIRR131 pKa = 11.84 FYY133 pKa = 9.89 YY134 pKa = 10.27 FKK136 pKa = 10.99 SKK138 pKa = 9.62 WSPVADD144 pKa = 3.3 VATGNVCGVNANFFDD159 pKa = 4.16 NSSSDD164 pKa = 3.35 TGFSGALMDD173 pKa = 4.37 LTRR176 pKa = 11.84 KK177 pKa = 9.14 IQSQNYY183 pKa = 8.28 TYY185 pKa = 10.91 LCHH188 pKa = 6.34 RR189 pKa = 11.84 TFKK192 pKa = 10.45 IGNSQPAAGMATSSIQANQTSNDD215 pKa = 3.46 FKK217 pKa = 10.64 MSMIGKK223 pKa = 8.94 VNLARR228 pKa = 11.84 FCKK231 pKa = 10.59 SKK233 pKa = 9.72 FTFNDD238 pKa = 2.97 ANQVMNPWIHH248 pKa = 7.16 CLMQVMAADD257 pKa = 4.55 GSVISTTQSQVSIQSQIYY275 pKa = 9.93 CEE277 pKa = 4.26 YY278 pKa = 10.21 TDD280 pKa = 3.6 EE281 pKa = 4.3
Molecular weight: 31.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.246
IPC2_protein 9.531
IPC_protein 9.589
Toseland 10.087
ProMoST 9.794
Dawson 10.306
Bjellqvist 9.999
Wikipedia 10.482
Rodwell 10.73
Grimsley 10.379
Solomon 10.321
Lehninger 10.292
Nozaki 10.131
DTASelect 9.984
Thurlkill 10.145
EMBOSS 10.496
Sillero 10.218
Patrickios 10.277
IPC_peptide 10.321
IPC2_peptide 8.902
IPC2.peptide.svr19 8.392
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
614
281
333
307.0
35.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.212 ± 0.543
2.28 ± 0.137
5.7 ± 1.387
4.56 ± 2.262
5.212 ± 0.149
4.397 ± 0.148
2.117 ± 0.68
6.515 ± 0.532
6.84 ± 0.179
5.537 ± 1.282
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.769 ± 0.281
5.537 ± 0.793
4.56 ± 0.188
4.723 ± 1.32
5.7 ± 0.226
8.306 ± 1.535
8.143 ± 0.718
5.863 ± 1.043
2.117 ± 0.91
3.909 ± 0.465
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here