Coccinia mosaic Virudhunagar virus
Average proteome isoelectric point is 7.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2D1P856|A0A2D1P856_9GEMI Replication enhancer protein OS=Coccinia mosaic Virudhunagar virus OX=2048880 PE=3 SV=1
MM1 pKa = 7.57 WARR4 pKa = 11.84 SLGTHH9 pKa = 6.76 NYY11 pKa = 10.22 LCGHH15 pKa = 7.39 LDD17 pKa = 4.41 LSPKK21 pKa = 10.0 IYY23 pKa = 11.0 SNDD26 pKa = 2.4 AWYY29 pKa = 10.92 NVIDD33 pKa = 5.2 DD34 pKa = 4.41 VDD36 pKa = 3.61 PHH38 pKa = 5.85 YY39 pKa = 11.0 LKK41 pKa = 10.7 HH42 pKa = 6.2 FKK44 pKa = 10.7 EE45 pKa = 4.46 FMGAQRR51 pKa = 11.84 DD52 pKa = 3.81 WQSNTKK58 pKa = 9.55 YY59 pKa = 10.51 GKK61 pKa = 9.02 PVHH64 pKa = 6.47 IKK66 pKa = 10.76 GGIPTIFLCNPGPSSSYY83 pKa = 10.22 IEE85 pKa = 4.02 YY86 pKa = 10.9 LEE88 pKa = 4.18 EE89 pKa = 4.03 EE90 pKa = 4.33 KK91 pKa = 11.12 NAALKK96 pKa = 10.42 AWALKK101 pKa = 9.94 NALFITLEE109 pKa = 4.31 GPLYY113 pKa = 10.98 SGGNQGATPEE123 pKa = 4.23 EE124 pKa = 4.59 QEE126 pKa = 4.64 SNPTQADD133 pKa = 3.14
Molecular weight: 14.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.178
IPC2_protein 5.308
IPC_protein 5.245
Toseland 5.461
ProMoST 5.258
Dawson 5.372
Bjellqvist 5.461
Wikipedia 5.308
Rodwell 5.321
Grimsley 5.512
Solomon 5.372
Lehninger 5.347
Nozaki 5.563
DTASelect 5.728
Thurlkill 5.575
EMBOSS 5.537
Sillero 5.652
Patrickios 3.325
IPC_peptide 5.385
IPC2_peptide 5.652
IPC2.peptide.svr19 5.673
Protein with the highest isoelectric point:
>tr|A0A2D1P853|A0A2D1P853_9GEMI Protein V2 OS=Coccinia mosaic Virudhunagar virus OX=2048880 PE=3 SV=1
MM1 pKa = 7.71 SKK3 pKa = 10.35 RR4 pKa = 11.84 PADD7 pKa = 3.87 IIISTPASKK16 pKa = 10.49 ARR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 LNFDD24 pKa = 2.97 RR25 pKa = 11.84 PYY27 pKa = 10.5 AARR30 pKa = 11.84 AVVPIVRR37 pKa = 11.84 GTKK40 pKa = 9.74 ARR42 pKa = 11.84 VWANRR47 pKa = 11.84 PMNRR51 pKa = 11.84 KK52 pKa = 8.06 PRR54 pKa = 11.84 IYY56 pKa = 10.77 RR57 pKa = 11.84 MFRR60 pKa = 11.84 SPDD63 pKa = 3.23 VPRR66 pKa = 11.84 GCEE69 pKa = 4.34 GPCKK73 pKa = 10.01 VQSFEE78 pKa = 4.06 SRR80 pKa = 11.84 HH81 pKa = 5.47 DD82 pKa = 3.38 VAHH85 pKa = 6.87 IGKK88 pKa = 9.09 VMCVSDD94 pKa = 3.59 VTRR97 pKa = 11.84 GTGLTHH103 pKa = 7.09 RR104 pKa = 11.84 VGKK107 pKa = 9.66 RR108 pKa = 11.84 FCVKK112 pKa = 9.94 SVYY115 pKa = 10.62 VLGKK119 pKa = 9.71 VWMDD123 pKa = 3.25 EE124 pKa = 4.06 NIKK127 pKa = 9.31 TKK129 pKa = 10.61 NHH131 pKa = 6.03 TNTVMFFLVRR141 pKa = 11.84 DD142 pKa = 3.75 RR143 pKa = 11.84 RR144 pKa = 11.84 PIDD147 pKa = 3.32 KK148 pKa = 9.56 PQDD151 pKa = 3.39 FGEE154 pKa = 4.33 VFNMFDD160 pKa = 4.29 NEE162 pKa = 4.11 PSTATVKK169 pKa = 10.61 NMHH172 pKa = 7.0 RR173 pKa = 11.84 DD174 pKa = 3.31 RR175 pKa = 11.84 YY176 pKa = 8.78 QVLRR180 pKa = 11.84 KK181 pKa = 8.43 WHH183 pKa = 5.6 ATVTGGTYY191 pKa = 10.43 ACRR194 pKa = 11.84 EE195 pKa = 3.76 QALVRR200 pKa = 11.84 KK201 pKa = 8.53 FIRR204 pKa = 11.84 VNNYY208 pKa = 7.74 VVYY211 pKa = 9.96 NQQEE215 pKa = 3.8 AGKK218 pKa = 10.1 YY219 pKa = 8.23 EE220 pKa = 4.02 NHH222 pKa = 6.47 TEE224 pKa = 3.99 NALMLYY230 pKa = 8.25 MASTHH235 pKa = 6.3 ASNPVYY241 pKa = 9.86 ATLKK245 pKa = 9.47 IRR247 pKa = 11.84 IYY249 pKa = 10.67 FYY251 pKa = 11.26 DD252 pKa = 3.51 SVSNN256 pKa = 3.9
Molecular weight: 29.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.309
IPC2_protein 9.648
IPC_protein 10.131
Toseland 10.335
ProMoST 10.028
Dawson 10.511
Bjellqvist 10.204
Wikipedia 10.701
Rodwell 10.818
Grimsley 10.57
Solomon 10.555
Lehninger 10.511
Nozaki 10.335
DTASelect 10.189
Thurlkill 10.365
EMBOSS 10.73
Sillero 10.423
Patrickios 10.452
IPC_peptide 10.555
IPC2_peptide 9.136
IPC2.peptide.svr19 8.598
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
1019
85
256
145.6
16.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.888 ± 0.558
2.552 ± 0.518
4.907 ± 0.393
4.318 ± 0.501
3.533 ± 0.693
5.496 ± 0.646
4.809 ± 0.871
4.514 ± 0.618
4.612 ± 0.966
7.655 ± 1.237
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.65 ± 0.493
4.809 ± 0.773
6.281 ± 0.671
3.827 ± 0.58
7.655 ± 1.02
7.066 ± 1.383
6.575 ± 1.081
7.458 ± 1.022
1.57 ± 0.314
3.827 ± 0.723
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here