Hungatella hathewayi WAL-18680

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Hungatella; Hungatella hathewayi

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5166 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G5IBN5|G5IBN5_9CLOT Pept_C1 domain-containing protein OS=Hungatella hathewayi WAL-18680 OX=742737 GN=HMPREF9473_00912 PE=4 SV=1
MM1 pKa = 7.52EE2 pKa = 4.84MLEE5 pKa = 4.3GADD8 pKa = 3.6VEE10 pKa = 4.36VLEE13 pKa = 5.55DD14 pKa = 4.46IEE16 pKa = 6.08IIDD19 pKa = 4.29EE20 pKa = 4.14NPEE23 pKa = 3.91IFDD26 pKa = 3.56EE27 pKa = 4.95DD28 pKa = 4.23YY29 pKa = 11.3VDD31 pKa = 4.48NVMDD35 pKa = 3.99SVTTEE40 pKa = 3.74TLEE43 pKa = 4.75FEE45 pKa = 4.54EE46 pKa = 4.93EE47 pKa = 3.87LDD49 pKa = 3.9NNVAEE54 pKa = 4.31ASEE57 pKa = 4.29IVEE60 pKa = 4.63MPDD63 pKa = 2.45IDD65 pKa = 4.28YY66 pKa = 10.42NQVYY70 pKa = 10.65AEE72 pKa = 4.51IEE74 pKa = 4.03EE75 pKa = 4.38EE76 pKa = 4.09LNQFDD81 pKa = 6.02FDD83 pKa = 5.22DD84 pKa = 5.39LDD86 pKa = 3.35IFKK89 pKa = 11.0NEE91 pKa = 3.46EE92 pKa = 4.12HH93 pKa = 7.23LNDD96 pKa = 4.5LLDD99 pKa = 4.84GFAQSKK105 pKa = 7.12WEE107 pKa = 4.08RR108 pKa = 11.84MDD110 pKa = 3.33TLEE113 pKa = 4.76RR114 pKa = 11.84KK115 pKa = 9.25EE116 pKa = 4.54VMDD119 pKa = 4.15NLADD123 pKa = 3.87YY124 pKa = 10.41VAEE127 pKa = 4.06AVGIEE132 pKa = 4.07HH133 pKa = 6.6TPDD136 pKa = 2.47ISFYY140 pKa = 10.61YY141 pKa = 10.43NPEE144 pKa = 3.85NGDD147 pKa = 3.36YY148 pKa = 11.45GEE150 pKa = 4.32YY151 pKa = 10.79NEE153 pKa = 5.51LDD155 pKa = 3.31NKK157 pKa = 10.5IKK159 pKa = 10.64INEE162 pKa = 4.1YY163 pKa = 10.83CLDD166 pKa = 4.31DD167 pKa = 4.59SNEE170 pKa = 3.89ALDD173 pKa = 4.24TVVHH177 pKa = 5.84EE178 pKa = 4.99LWHH181 pKa = 5.94SYY183 pKa = 7.4QRR185 pKa = 11.84QRR187 pKa = 11.84ASNPQTAKK195 pKa = 10.75DD196 pKa = 3.51YY197 pKa = 10.37QYY199 pKa = 11.22QYY201 pKa = 11.71NFDD204 pKa = 4.2HH205 pKa = 6.83YY206 pKa = 10.71IEE208 pKa = 4.63PLKK211 pKa = 11.1GPDD214 pKa = 3.34GNYY217 pKa = 10.24VNIINYY223 pKa = 8.94EE224 pKa = 4.0EE225 pKa = 3.95QLVEE229 pKa = 4.0VEE231 pKa = 4.18ARR233 pKa = 11.84AFAAHH238 pKa = 6.13FRR240 pKa = 11.84EE241 pKa = 4.67KK242 pKa = 10.31MRR244 pKa = 11.84TT245 pKa = 3.39

Molecular weight: 28.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G5I979|G5I979_9CLOT Uncharacterized protein OS=Hungatella hathewayi WAL-18680 OX=742737 GN=HMPREF9473_00069 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.41KK9 pKa = 7.5RR10 pKa = 11.84QRR12 pKa = 11.84ARR14 pKa = 11.84VHH16 pKa = 6.02GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSPGGRR28 pKa = 11.84KK29 pKa = 8.91VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.33GRR39 pKa = 11.84AKK41 pKa = 10.69LSAA44 pKa = 3.92

Molecular weight: 4.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5166

0

5166

1708591

21

4185

330.7

36.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.865 ± 0.038

1.451 ± 0.013

5.405 ± 0.028

7.515 ± 0.045

3.939 ± 0.03

8.011 ± 0.058

1.686 ± 0.017

6.84 ± 0.034

5.676 ± 0.033

8.948 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.287 ± 0.023

4.13 ± 0.024

3.532 ± 0.021

3.232 ± 0.02

4.617 ± 0.031

5.97 ± 0.034

5.634 ± 0.036

7.115 ± 0.032

1.044 ± 0.012

4.104 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski