Amino acid dipepetide frequency for Firmicutes bacterium CAG:194

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.62AlaAla: 7.62 ± 0.16	1.18AlaCys: 1.18 ± 0.045	5.473AlaAsp: 5.473 ± 0.096	5.99AlaGlu: 5.99 ± 0.119	3.254AlaPhe: 3.254 ± 0.079	6.421AlaGly: 6.421 ± 0.108	1.165AlaHis: 1.165 ± 0.04	4.972AlaIle: 4.972 ± 0.097	5.368AlaLys: 5.368 ± 0.096	7.091AlaLeu: 7.091 ± 0.102	2.529AlaMet: 2.529 ± 0.061	2.632AlaAsn: 2.632 ± 0.065	2.013AlaPro: 2.013 ± 0.055	2.767AlaGln: 2.767 ± 0.076	2.808AlaArg: 2.808 ± 0.063	4.032AlaSer: 4.032 ± 0.086	3.649AlaThr: 3.649 ± 0.096	6.434AlaVal: 6.434 ± 0.099	0.559AlaTrp: 0.559 ± 0.025	3.348AlaTyr: 3.348 ± 0.069	0.0AlaXaa: 0.0 ± 0.0
Cys	0.961CysAla: 0.961 ± 0.038	0.291CysCys: 0.291 ± 0.023	0.842CysAsp: 0.842 ± 0.036	0.883CysGlu: 0.883 ± 0.039	0.717CysPhe: 0.717 ± 0.033	1.412CysGly: 1.412 ± 0.043	0.303CysHis: 0.303 ± 0.02	1.183CysIle: 1.183 ± 0.046	0.883CysLys: 0.883 ± 0.035	1.284CysLeu: 1.284 ± 0.043	0.531CysMet: 0.531 ± 0.027	0.587CysAsn: 0.587 ± 0.03	0.557CysPro: 0.557 ± 0.03	0.444CysGln: 0.444 ± 0.025	0.672CysArg: 0.672 ± 0.034	0.891CysSer: 0.891 ± 0.033	0.635CysThr: 0.635 ± 0.028	1.079CysVal: 1.079 ± 0.034	0.109CysTrp: 0.109 ± 0.011	0.6CysTyr: 0.6 ± 0.031	0.0CysXaa: 0.0 ± 0.0
Asp	4.946AspAla: 4.946 ± 0.095	0.84AspCys: 0.84 ± 0.036	2.896AspAsp: 2.896 ± 0.086	4.455AspGlu: 4.455 ± 0.092	2.533AspPhe: 2.533 ± 0.06	4.518AspGly: 4.518 ± 0.102	1.043AspHis: 1.043 ± 0.039	4.285AspIle: 4.285 ± 0.082	3.52AspLys: 3.52 ± 0.084	4.872AspLeu: 4.872 ± 0.079	1.933AspMet: 1.933 ± 0.051	2.054AspAsn: 2.054 ± 0.05	1.931AspPro: 1.931 ± 0.055	1.639AspGln: 1.639 ± 0.053	2.515AspArg: 2.515 ± 0.063	3.027AspSer: 3.027 ± 0.07	3.365AspThr: 3.365 ± 0.076	3.864AspVal: 3.864 ± 0.074	0.532AspTrp: 0.532 ± 0.031	2.878AspTyr: 2.878 ± 0.065	0.0AspXaa: 0.0 ± 0.0
Glu	5.855GluAla: 5.855 ± 0.11	0.711GluCys: 0.711 ± 0.029	4.338GluAsp: 4.338 ± 0.086	7.081GluGlu: 7.081 ± 0.143	2.114GluPhe: 2.114 ± 0.056	4.109GluGly: 4.109 ± 0.076	1.223GluHis: 1.223 ± 0.04	5.564GluIle: 5.564 ± 0.085	7.256GluLys: 7.256 ± 0.122	6.286GluLeu: 6.286 ± 0.094	2.438GluMet: 2.438 ± 0.062	4.095GluAsn: 4.095 ± 0.077	1.838GluPro: 1.838 ± 0.057	3.402GluGln: 3.402 ± 0.087	3.011GluArg: 3.011 ± 0.072	3.297GluSer: 3.297 ± 0.07	4.109GluThr: 4.109 ± 0.082	4.266GluVal: 4.266 ± 0.087	0.524GluTrp: 0.524 ± 0.028	2.714GluTyr: 2.714 ± 0.067	0.0GluXaa: 0.0 ± 0.0
Phe	3.204PheAla: 3.204 ± 0.069	0.744PheCys: 0.744 ± 0.036	2.452PheAsp: 2.452 ± 0.064	2.459PheGlu: 2.459 ± 0.06	1.855PhePhe: 1.855 ± 0.065	2.857PheGly: 2.857 ± 0.068	0.834PheHis: 0.834 ± 0.034	2.569PheIle: 2.569 ± 0.069	1.785PheLys: 1.785 ± 0.054	4.08PheLeu: 4.08 ± 0.098	1.227PheMet: 1.227 ± 0.038	1.239PheAsn: 1.239 ± 0.046	1.255PhePro: 1.255 ± 0.046	1.234PheGln: 1.234 ± 0.047	1.577PheArg: 1.577 ± 0.048	2.713PheSer: 2.713 ± 0.072	2.371PheThr: 2.371 ± 0.064	2.848PheVal: 2.848 ± 0.066	0.375PheTrp: 0.375 ± 0.02	1.793PheTyr: 1.793 ± 0.053	0.0PheXaa: 0.0 ± 0.0
Gly	4.858GlyAla: 4.858 ± 0.103	1.246GlyCys: 1.246 ± 0.044	3.458GlyAsp: 3.458 ± 0.066	4.519GlyGlu: 4.519 ± 0.078	3.006GlyPhe: 3.006 ± 0.067	4.41GlyGly: 4.41 ± 0.114	1.185GlyHis: 1.185 ± 0.04	6.111GlyIle: 6.111 ± 0.109	5.561GlyLys: 5.561 ± 0.096	5.629GlyLeu: 5.629 ± 0.102	2.474GlyMet: 2.474 ± 0.057	3.152GlyAsn: 3.152 ± 0.081	1.166GlyPro: 1.166 ± 0.043	2.515GlyGln: 2.515 ± 0.062	2.945GlyArg: 2.945 ± 0.066	4.206GlySer: 4.206 ± 0.085	4.082GlyThr: 4.082 ± 0.087	4.61GlyVal: 4.61 ± 0.084	0.609GlyTrp: 0.609 ± 0.035	3.366GlyTyr: 3.366 ± 0.071	0.0GlyXaa: 0.0 ± 0.0
His	1.213HisAla: 1.213 ± 0.041	0.288HisCys: 0.288 ± 0.02	0.947HisAsp: 0.947 ± 0.04	1.024HisGlu: 1.024 ± 0.038	0.883HisPhe: 0.883 ± 0.038	1.209HisGly: 1.209 ± 0.044	0.363HisHis: 0.363 ± 0.025	1.385HisIle: 1.385 ± 0.044	1.133HisLys: 1.133 ± 0.043	1.432HisLeu: 1.432 ± 0.041	0.639HisMet: 0.639 ± 0.028	0.681HisAsn: 0.681 ± 0.033	0.802HisPro: 0.802 ± 0.036	0.478HisGln: 0.478 ± 0.022	0.673HisArg: 0.673 ± 0.033	0.913HisSer: 0.913 ± 0.037	1.037HisThr: 1.037 ± 0.038	1.179HisVal: 1.179 ± 0.04	0.126HisTrp: 0.126 ± 0.013	0.783HisTyr: 0.783 ± 0.033	0.0HisXaa: 0.0 ± 0.0
Ile	5.964IleAla: 5.964 ± 0.098	1.449IleCys: 1.449 ± 0.045	4.118IleAsp: 4.118 ± 0.09	4.682IleGlu: 4.682 ± 0.084	2.68IlePhe: 2.68 ± 0.072	5.255IleGly: 5.255 ± 0.114	1.313IleHis: 1.313 ± 0.043	4.713IleIle: 4.713 ± 0.101	3.948IleLys: 3.948 ± 0.079	7.015IleLeu: 7.015 ± 0.131	1.957IleMet: 1.957 ± 0.058	2.664IleAsn: 2.664 ± 0.063	2.985IlePro: 2.985 ± 0.062	2.025IleGln: 2.025 ± 0.053	3.769IleArg: 3.769 ± 0.078	4.678IleSer: 4.678 ± 0.097	4.387IleThr: 4.387 ± 0.08	5.018IleVal: 5.018 ± 0.091	0.633IleTrp: 0.633 ± 0.034	2.937IleTyr: 2.937 ± 0.061	0.001IleXaa: 0.001 ± 0.001
Lys	5.312LysAla: 5.312 ± 0.089	0.74LysCys: 0.74 ± 0.037	3.955LysAsp: 3.955 ± 0.085	7.013LysGlu: 7.013 ± 0.114	1.759LysPhe: 1.759 ± 0.048	4.069LysGly: 4.069 ± 0.081	0.98LysHis: 0.98 ± 0.033	4.703LysIle: 4.703 ± 0.083	6.903LysLys: 6.903 ± 0.14	5.884LysLeu: 5.884 ± 0.094	2.207LysMet: 2.207 ± 0.059	3.618LysAsn: 3.618 ± 0.069	2.106LysPro: 2.106 ± 0.063	3.014LysGln: 3.014 ± 0.072	2.764LysArg: 2.764 ± 0.065	3.197LysSer: 3.197 ± 0.069	3.955LysThr: 3.955 ± 0.083	4.505LysVal: 4.505 ± 0.086	0.595LysTrp: 0.595 ± 0.031	2.793LysTyr: 2.793 ± 0.066	0.0LysXaa: 0.0 ± 0.0
Leu	7.359LeuAla: 7.359 ± 0.118	1.599LeuCys: 1.599 ± 0.05	4.885LeuAsp: 4.885 ± 0.08	5.523LeuGlu: 5.523 ± 0.101	4.062LeuPhe: 4.062 ± 0.113	5.714LeuGly: 5.714 ± 0.099	1.729LeuHis: 1.729 ± 0.051	5.892LeuIle: 5.892 ± 0.108	5.726LeuLys: 5.726 ± 0.09	10.099LeuLeu: 10.099 ± 0.205	2.531LeuMet: 2.531 ± 0.061	3.212LeuAsn: 3.212 ± 0.067	3.703LeuPro: 3.703 ± 0.083	3.733LeuGln: 3.733 ± 0.083	3.549LeuArg: 3.549 ± 0.071	6.481LeuSer: 6.481 ± 0.102	5.724LeuThr: 5.724 ± 0.104	5.726LeuVal: 5.726 ± 0.096	0.73LeuTrp: 0.73 ± 0.026	3.729LeuTyr: 3.729 ± 0.082	0.0LeuXaa: 0.0 ± 0.0
Met	2.591MetAla: 2.591 ± 0.062	0.352MetCys: 0.352 ± 0.022	1.81MetAsp: 1.81 ± 0.044	2.607MetGlu: 2.607 ± 0.063	0.95MetPhe: 0.95 ± 0.041	2.062MetGly: 2.062 ± 0.052	0.58MetHis: 0.58 ± 0.029	2.298MetIle: 2.298 ± 0.057	2.368MetLys: 2.368 ± 0.067	2.933MetLeu: 2.933 ± 0.066	0.913MetMet: 0.913 ± 0.036	1.416MetAsn: 1.416 ± 0.04	1.176MetPro: 1.176 ± 0.047	1.637MetGln: 1.637 ± 0.056	1.231MetArg: 1.231 ± 0.042	1.654MetSer: 1.654 ± 0.044	1.842MetThr: 1.842 ± 0.052	1.899MetVal: 1.899 ± 0.047	0.249MetTrp: 0.249 ± 0.017	0.879MetTyr: 0.879 ± 0.035	0.0MetXaa: 0.0 ± 0.0
Asn	3.372AsnAla: 3.372 ± 0.065	0.616AsnCys: 0.616 ± 0.033	2.011AsnAsp: 2.011 ± 0.054	2.65AsnGlu: 2.65 ± 0.054	1.386AsnPhe: 1.386 ± 0.046	3.521AsnGly: 3.521 ± 0.079	0.722AsnHis: 0.722 ± 0.031	3.061AsnIle: 3.061 ± 0.074	2.557AsnLys: 2.557 ± 0.058	3.504AsnLeu: 3.504 ± 0.07	1.365AsnMet: 1.365 ± 0.044	1.637AsnAsn: 1.637 ± 0.066	1.766AsnPro: 1.766 ± 0.047	1.349AsnGln: 1.349 ± 0.047	1.961AsnArg: 1.961 ± 0.051	2.067AsnSer: 2.067 ± 0.063	2.282AsnThr: 2.282 ± 0.055	2.818AsnVal: 2.818 ± 0.067	0.368AsnTrp: 0.368 ± 0.022	1.723AsnTyr: 1.723 ± 0.052	0.0AsnXaa: 0.0 ± 0.0
Pro	2.662ProAla: 2.662 ± 0.073	0.438ProCys: 0.438 ± 0.027	2.375ProAsp: 2.375 ± 0.06	2.772ProGlu: 2.772 ± 0.071	1.42ProPhe: 1.42 ± 0.045	2.188ProGly: 2.188 ± 0.049	0.534ProHis: 0.534 ± 0.027	2.043ProIle: 2.043 ± 0.05	1.909ProLys: 1.909 ± 0.052	2.639ProLeu: 2.639 ± 0.064	0.787ProMet: 0.787 ± 0.035	1.049ProAsn: 1.049 ± 0.037	0.61ProPro: 0.61 ± 0.03	1.269ProGln: 1.269 ± 0.051	0.903ProArg: 0.903 ± 0.038	1.668ProSer: 1.668 ± 0.06	1.421ProThr: 1.421 ± 0.047	3.031ProVal: 3.031 ± 0.059	0.267ProTrp: 0.267 ± 0.019	1.542ProTyr: 1.542 ± 0.052	0.0ProXaa: 0.0 ± 0.0
Gln	3.022GlnAla: 3.022 ± 0.08	0.317GlnCys: 0.317 ± 0.02	1.77GlnAsp: 1.77 ± 0.049	3.091GlnGlu: 3.091 ± 0.067	1.174GlnPhe: 1.174 ± 0.042	2.024GlnGly: 2.024 ± 0.05	0.54GlnHis: 0.54 ± 0.027	3.124GlnIle: 3.124 ± 0.069	3.349GlnLys: 3.349 ± 0.072	3.325GlnLeu: 3.325 ± 0.073	1.326GlnMet: 1.326 ± 0.049	1.77GlnAsn: 1.77 ± 0.056	1.03GlnPro: 1.03 ± 0.04	1.696GlnGln: 1.696 ± 0.068	1.299GlnArg: 1.299 ± 0.044	1.77GlnSer: 1.77 ± 0.054	2.337GlnThr: 2.337 ± 0.067	2.249GlnVal: 2.249 ± 0.056	0.216GlnTrp: 0.216 ± 0.017	1.517GlnTyr: 1.517 ± 0.049	0.0GlnXaa: 0.0 ± 0.0
Arg	2.643ArgAla: 2.643 ± 0.057	0.551ArgCys: 0.551 ± 0.028	2.055ArgAsp: 2.055 ± 0.058	3.499ArgGlu: 3.499 ± 0.073	1.63ArgPhe: 1.63 ± 0.048	2.254ArgGly: 2.254 ± 0.062	0.656ArgHis: 0.656 ± 0.032	3.467ArgIle: 3.467 ± 0.076	3.425ArgLys: 3.425 ± 0.07	3.674ArgLeu: 3.674 ± 0.077	1.535ArgMet: 1.535 ± 0.047	1.863ArgAsn: 1.863 ± 0.052	1.144ArgPro: 1.144 ± 0.043	1.854ArgGln: 1.854 ± 0.058	1.977ArgArg: 1.977 ± 0.057	2.138ArgSer: 2.138 ± 0.06	2.063ArgThr: 2.063 ± 0.054	2.469ArgVal: 2.469 ± 0.052	0.274ArgTrp: 0.274 ± 0.019	1.775ArgTyr: 1.775 ± 0.051	0.0ArgXaa: 0.0 ± 0.0
Ser	4.472SerAla: 4.472 ± 0.089	0.823SerCys: 0.823 ± 0.035	3.482SerAsp: 3.482 ± 0.079	3.428SerGlu: 3.428 ± 0.078	2.605SerPhe: 2.605 ± 0.062	4.502SerGly: 4.502 ± 0.082	0.998SerHis: 0.998 ± 0.037	3.907SerIle: 3.907 ± 0.084	3.173SerLys: 3.173 ± 0.066	5.192SerLeu: 5.192 ± 0.099	1.99SerMet: 1.99 ± 0.056	2.02SerAsn: 2.02 ± 0.054	1.561SerPro: 1.561 ± 0.049	1.797SerGln: 1.797 ± 0.053	2.316SerArg: 2.316 ± 0.053	3.224SerSer: 3.224 ± 0.072	2.681SerThr: 2.681 ± 0.058	4.446SerVal: 4.446 ± 0.076	0.457SerTrp: 0.457 ± 0.028	2.849SerTyr: 2.849 ± 0.064	0.0SerXaa: 0.0 ± 0.0
Thr	4.693ThrAla: 4.693 ± 0.084	0.694ThrCys: 0.694 ± 0.032	3.808ThrAsp: 3.808 ± 0.084	4.333ThrGlu: 4.333 ± 0.084	2.228ThrPhe: 2.228 ± 0.054	4.923ThrGly: 4.923 ± 0.093	0.875ThrHis: 0.875 ± 0.035	4.1ThrIle: 4.1 ± 0.091	3.651ThrLys: 3.651 ± 0.074	4.796ThrLeu: 4.796 ± 0.097	1.561ThrMet: 1.561 ± 0.047	1.992ThrAsn: 1.992 ± 0.055	1.996ThrPro: 1.996 ± 0.062	1.696ThrGln: 1.696 ± 0.054	1.799ThrArg: 1.799 ± 0.053	2.806ThrSer: 2.806 ± 0.071	3.072ThrThr: 3.072 ± 0.083	4.667ThrVal: 4.667 ± 0.096	0.426ThrTrp: 0.426 ± 0.029	2.392ThrTyr: 2.392 ± 0.059	0.0ThrXaa: 0.0 ± 0.0
Val	4.967ValAla: 4.967 ± 0.079	1.264ValCys: 1.264 ± 0.039	3.797ValAsp: 3.797 ± 0.068	4.397ValGlu: 4.397 ± 0.076	3.006ValPhe: 3.006 ± 0.078	4.099ValGly: 4.099 ± 0.074	1.09ValHis: 1.09 ± 0.035	5.266ValIle: 5.266 ± 0.084	4.527ValLys: 4.527 ± 0.089	7.001ValLeu: 7.001 ± 0.103	2.072ValMet: 2.072 ± 0.058	2.768ValAsn: 2.768 ± 0.06	2.266ValPro: 2.266 ± 0.06	2.233ValGln: 2.233 ± 0.055	2.828ValArg: 2.828 ± 0.058	4.768ValSer: 4.768 ± 0.086	4.611ValThr: 4.611 ± 0.085	4.741ValVal: 4.741 ± 0.088	0.529ValTrp: 0.529 ± 0.03	2.924ValTyr: 2.924 ± 0.069	0.0ValXaa: 0.0 ± 0.0
Trp	0.465TrpAla: 0.465 ± 0.028	0.122TrpCys: 0.122 ± 0.012	0.458TrpAsp: 0.458 ± 0.027	0.532TrpGlu: 0.532 ± 0.028	0.324TrpPhe: 0.324 ± 0.023	0.61TrpGly: 0.61 ± 0.032	0.17TrpHis: 0.17 ± 0.018	0.641TrpIle: 0.641 ± 0.03	0.651TrpLys: 0.651 ± 0.029	0.799TrpLeu: 0.799 ± 0.034	0.233TrpMet: 0.233 ± 0.016	0.465TrpAsn: 0.465 ± 0.025	0.176TrpPro: 0.176 ± 0.015	0.414TrpGln: 0.414 ± 0.023	0.305TrpArg: 0.305 ± 0.021	0.385TrpSer: 0.385 ± 0.022	0.354TrpThr: 0.354 ± 0.022	0.411TrpVal: 0.411 ± 0.023	0.092TrpTrp: 0.092 ± 0.011	0.362TrpTyr: 0.362 ± 0.025	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.124TyrAla: 3.124 ± 0.066	0.597TyrCys: 0.597 ± 0.03	2.714TyrAsp: 2.714 ± 0.07	3.296TyrGlu: 3.296 ± 0.059	1.886TyrPhe: 1.886 ± 0.044	2.951TyrGly: 2.951 ± 0.071	0.885TyrHis: 0.885 ± 0.039	2.907TyrIle: 2.907 ± 0.06	2.379TyrLys: 2.379 ± 0.062	4.171TyrLeu: 4.171 ± 0.087	1.197TyrMet: 1.197 ± 0.041	1.821TyrAsn: 1.821 ± 0.052	1.446TyrPro: 1.446 ± 0.051	1.747TyrGln: 1.747 ± 0.053	2.045TyrArg: 2.045 ± 0.058	2.009TyrSer: 2.009 ± 0.057	2.531TyrThr: 2.531 ± 0.073	2.895TyrVal: 2.895 ± 0.063	0.3TyrTrp: 0.3 ± 0.024	2.16TyrTyr: 2.16 ± 0.055	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2310 proteins (763414 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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