Amino acid dipepetide frequency for Pseudomonas sp. 5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	12.45AlaAla: 12.45 ± 0.125	1.276AlaCys: 1.276 ± 0.029	5.786AlaAsp: 5.786 ± 0.057	6.848AlaGlu: 6.848 ± 0.08	3.814AlaPhe: 3.814 ± 0.056	8.616AlaGly: 8.616 ± 0.081	2.225AlaHis: 2.225 ± 0.043	5.104AlaIle: 5.104 ± 0.065	3.745AlaLys: 3.745 ± 0.06	13.963AlaLeu: 13.963 ± 0.121	2.881AlaMet: 2.881 ± 0.042	3.153AlaAsn: 3.153 ± 0.045	4.69AlaPro: 4.69 ± 0.067	5.743AlaGln: 5.743 ± 0.07	6.877AlaArg: 6.877 ± 0.08	6.34AlaSer: 6.34 ± 0.064	4.701AlaThr: 4.701 ± 0.055	7.614AlaVal: 7.614 ± 0.081	1.595AlaTrp: 1.595 ± 0.037	2.555AlaTyr: 2.555 ± 0.048	0.001AlaXaa: 0.001 ± 0.001
Cys	1.134CysAla: 1.134 ± 0.027	0.146CysCys: 0.146 ± 0.008	0.565CysAsp: 0.565 ± 0.019	0.578CysGlu: 0.578 ± 0.019	0.355CysPhe: 0.355 ± 0.016	0.903CysGly: 0.903 ± 0.024	0.301CysHis: 0.301 ± 0.016	0.446CysIle: 0.446 ± 0.016	0.293CysLys: 0.293 ± 0.014	1.199CysLeu: 1.199 ± 0.031	0.215CysMet: 0.215 ± 0.012	0.282CysAsn: 0.282 ± 0.013	0.508CysPro: 0.508 ± 0.021	0.473CysGln: 0.473 ± 0.018	0.619CysArg: 0.619 ± 0.02	0.64CysSer: 0.64 ± 0.022	0.506CysThr: 0.506 ± 0.019	0.758CysVal: 0.758 ± 0.025	0.166CysTrp: 0.166 ± 0.01	0.259CysTyr: 0.259 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	5.547AspAla: 5.547 ± 0.059	0.579AspCys: 0.579 ± 0.021	2.893AspAsp: 2.893 ± 0.057	3.317AspGlu: 3.317 ± 0.048	2.121AspPhe: 2.121 ± 0.034	4.27AspGly: 4.27 ± 0.061	1.186AspHis: 1.186 ± 0.027	2.543AspIle: 2.543 ± 0.044	1.903AspLys: 1.903 ± 0.037	5.977AspLeu: 5.977 ± 0.059	1.19AspMet: 1.19 ± 0.028	1.729AspAsn: 1.729 ± 0.036	2.837AspPro: 2.837 ± 0.048	2.341AspGln: 2.341 ± 0.04	2.978AspArg: 2.978 ± 0.052	3.044AspSer: 3.044 ± 0.046	2.411AspThr: 2.411 ± 0.04	3.503AspVal: 3.503 ± 0.052	0.956AspTrp: 0.956 ± 0.026	1.757AspTyr: 1.757 ± 0.037	0.0AspXaa: 0.0 ± 0.0
Glu	6.219GluAla: 6.219 ± 0.077	0.443GluCys: 0.443 ± 0.017	2.512GluAsp: 2.512 ± 0.036	2.996GluGlu: 2.996 ± 0.057	1.749GluPhe: 1.749 ± 0.035	4.021GluGly: 4.021 ± 0.053	1.755GluHis: 1.755 ± 0.035	2.605GluIle: 2.605 ± 0.042	1.997GluLys: 1.997 ± 0.047	6.987GluLeu: 6.987 ± 0.071	1.269GluMet: 1.269 ± 0.03	1.476GluAsn: 1.476 ± 0.033	2.46GluPro: 2.46 ± 0.041	4.205GluGln: 4.205 ± 0.062	4.771GluArg: 4.771 ± 0.067	2.623GluSer: 2.623 ± 0.04	2.409GluThr: 2.409 ± 0.038	4.234GluVal: 4.234 ± 0.058	0.696GluTrp: 0.696 ± 0.025	1.196GluTyr: 1.196 ± 0.031	0.0GluXaa: 0.0 ± 0.0
Phe	4.062PheAla: 4.062 ± 0.051	0.488PheCys: 0.488 ± 0.016	2.476PheAsp: 2.476 ± 0.035	2.11PheGlu: 2.11 ± 0.035	1.509PhePhe: 1.509 ± 0.035	3.196PheGly: 3.196 ± 0.054	0.719PheHis: 0.719 ± 0.02	1.892PheIle: 1.892 ± 0.037	1.322PheLys: 1.322 ± 0.027	3.209PheLeu: 3.209 ± 0.051	0.821PheMet: 0.821 ± 0.024	1.427PheAsn: 1.427 ± 0.028	1.414PhePro: 1.414 ± 0.031	1.183PheGln: 1.183 ± 0.025	1.728PheArg: 1.728 ± 0.037	2.56PheSer: 2.56 ± 0.045	1.904PheThr: 1.904 ± 0.038	2.528PheVal: 2.528 ± 0.038	0.566PheTrp: 0.566 ± 0.021	1.067PheTyr: 1.067 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	7.293GlyAla: 7.293 ± 0.08	0.951GlyCys: 0.951 ± 0.029	3.879GlyAsp: 3.879 ± 0.052	4.577GlyGlu: 4.577 ± 0.058	3.306GlyPhe: 3.306 ± 0.047	5.952GlyGly: 5.952 ± 0.089	1.871GlyHis: 1.871 ± 0.037	4.065GlyIle: 4.065 ± 0.051	3.363GlyLys: 3.363 ± 0.049	9.43GlyLeu: 9.43 ± 0.089	2.171GlyMet: 2.171 ± 0.039	2.465GlyAsn: 2.465 ± 0.048	2.612GlyPro: 2.612 ± 0.038	3.843GlyGln: 3.843 ± 0.056	4.787GlyArg: 4.787 ± 0.052	4.659GlySer: 4.659 ± 0.058	3.64GlyThr: 3.64 ± 0.058	6.044GlyVal: 6.044 ± 0.063	1.356GlyTrp: 1.356 ± 0.03	2.417GlyTyr: 2.417 ± 0.045	0.0GlyXaa: 0.0 ± 0.0
His	2.273HisAla: 2.273 ± 0.039	0.356HisCys: 0.356 ± 0.016	1.232HisAsp: 1.232 ± 0.027	1.228HisGlu: 1.228 ± 0.028	1.005HisPhe: 1.005 ± 0.027	1.956HisGly: 1.956 ± 0.042	0.607HisHis: 0.607 ± 0.019	0.995HisIle: 0.995 ± 0.024	0.676HisLys: 0.676 ± 0.021	2.634HisLeu: 2.634 ± 0.05	0.529HisMet: 0.529 ± 0.015	0.715HisAsn: 0.715 ± 0.023	1.384HisPro: 1.384 ± 0.029	1.052HisGln: 1.052 ± 0.028	1.279HisArg: 1.279 ± 0.029	1.335HisSer: 1.335 ± 0.033	0.976HisThr: 0.976 ± 0.022	1.391HisVal: 1.391 ± 0.027	0.499HisTrp: 0.499 ± 0.022	0.801HisTyr: 0.801 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	5.845IleAla: 5.845 ± 0.067	0.519IleCys: 0.519 ± 0.02	3.315IleAsp: 3.315 ± 0.048	3.301IleGlu: 3.301 ± 0.052	1.416IlePhe: 1.416 ± 0.034	4.475IleGly: 4.475 ± 0.056	0.905IleHis: 0.905 ± 0.027	2.072IleIle: 2.072 ± 0.041	1.744IleLys: 1.744 ± 0.034	4.062IleLeu: 4.062 ± 0.049	0.806IleMet: 0.806 ± 0.024	1.8IleAsn: 1.8 ± 0.033	2.174IlePro: 2.174 ± 0.04	1.593IleGln: 1.593 ± 0.032	2.734IleArg: 2.734 ± 0.04	3.028IleSer: 3.028 ± 0.047	2.554IleThr: 2.554 ± 0.043	3.156IleVal: 3.156 ± 0.05	0.506IleTrp: 0.506 ± 0.018	1.113IleTyr: 1.113 ± 0.025	0.0IleXaa: 0.0 ± 0.0
Lys	4.226LysAla: 4.226 ± 0.065	0.188LysCys: 0.188 ± 0.01	1.82LysAsp: 1.82 ± 0.036	1.684LysGlu: 1.684 ± 0.038	0.883LysPhe: 0.883 ± 0.025	2.599LysGly: 2.599 ± 0.045	0.794LysHis: 0.794 ± 0.02	1.566LysIle: 1.566 ± 0.034	1.245LysLys: 1.245 ± 0.037	3.704LysLeu: 3.704 ± 0.051	0.714LysMet: 0.714 ± 0.026	0.988LysAsn: 0.988 ± 0.027	2.032LysPro: 2.032 ± 0.044	1.629LysGln: 1.629 ± 0.031	2.422LysArg: 2.422 ± 0.038	1.768LysSer: 1.768 ± 0.037	1.83LysThr: 1.83 ± 0.034	2.921LysVal: 2.921 ± 0.048	0.344LysTrp: 0.344 ± 0.014	0.732LysTyr: 0.732 ± 0.022	0.0LysXaa: 0.0 ± 0.0
Leu	14.283LeuAla: 14.283 ± 0.115	1.301LeuCys: 1.301 ± 0.028	6.682LeuAsp: 6.682 ± 0.069	6.652LeuGlu: 6.652 ± 0.072	4.244LeuPhe: 4.244 ± 0.055	9.37LeuGly: 9.37 ± 0.077	2.566LeuHis: 2.566 ± 0.047	5.653LeuIle: 5.653 ± 0.075	4.46LeuLys: 4.46 ± 0.066	13.775LeuLeu: 13.775 ± 0.156	2.597LeuMet: 2.597 ± 0.041	3.8LeuAsn: 3.8 ± 0.045	6.271LeuPro: 6.271 ± 0.077	5.261LeuGln: 5.261 ± 0.066	7.425LeuArg: 7.425 ± 0.088	7.583LeuSer: 7.583 ± 0.081	5.829LeuThr: 5.829 ± 0.058	7.83LeuVal: 7.83 ± 0.074	1.415LeuTrp: 1.415 ± 0.033	2.731LeuTyr: 2.731 ± 0.045	0.0LeuXaa: 0.0 ± 0.0
Met	2.686MetAla: 2.686 ± 0.047	0.168MetCys: 0.168 ± 0.01	1.001MetAsp: 1.001 ± 0.025	0.91MetGlu: 0.91 ± 0.024	0.74MetPhe: 0.74 ± 0.023	1.713MetGly: 1.713 ± 0.033	0.501MetHis: 0.501 ± 0.017	1.145MetIle: 1.145 ± 0.027	0.843MetLys: 0.843 ± 0.023	2.765MetLeu: 2.765 ± 0.044	0.463MetMet: 0.463 ± 0.017	0.856MetAsn: 0.856 ± 0.024	1.342MetPro: 1.342 ± 0.026	1.039MetGln: 1.039 ± 0.024	1.512MetArg: 1.512 ± 0.029	1.727MetSer: 1.727 ± 0.033	1.392MetThr: 1.392 ± 0.03	1.566MetVal: 1.566 ± 0.032	0.18MetTrp: 0.18 ± 0.011	0.388MetTyr: 0.388 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	3.291AsnAla: 3.291 ± 0.047	0.313AsnCys: 0.313 ± 0.013	1.687AsnAsp: 1.687 ± 0.039	1.548AsnGlu: 1.548 ± 0.032	1.039AsnPhe: 1.039 ± 0.026	2.579AsnGly: 2.579 ± 0.044	0.688AsnHis: 0.688 ± 0.021	1.413AsnIle: 1.413 ± 0.031	0.987AsnLys: 0.987 ± 0.028	3.649AsnLeu: 3.649 ± 0.056	0.594AsnMet: 0.594 ± 0.015	1.023AsnAsn: 1.023 ± 0.031	1.967AsnPro: 1.967 ± 0.039	1.425AsnGln: 1.425 ± 0.032	1.925AsnArg: 1.925 ± 0.032	1.69AsnSer: 1.69 ± 0.036	1.564AsnThr: 1.564 ± 0.033	2.009AsnVal: 2.009 ± 0.043	0.496AsnTrp: 0.496 ± 0.016	0.879AsnTyr: 0.879 ± 0.028	0.0AsnXaa: 0.0 ± 0.0
Pro	5.483ProAla: 5.483 ± 0.071	0.389ProCys: 0.389 ± 0.017	2.659ProAsp: 2.659 ± 0.041	3.126ProGlu: 3.126 ± 0.045	1.788ProPhe: 1.788 ± 0.04	3.99ProGly: 3.99 ± 0.048	1.039ProHis: 1.039 ± 0.025	2.001ProIle: 2.001 ± 0.038	1.511ProLys: 1.511 ± 0.031	5.823ProLeu: 5.823 ± 0.064	1.117ProMet: 1.117 ± 0.029	1.374ProAsn: 1.374 ± 0.03	1.805ProPro: 1.805 ± 0.041	2.458ProGln: 2.458 ± 0.037	2.567ProArg: 2.567 ± 0.044	2.59ProSer: 2.59 ± 0.041	2.176ProThr: 2.176 ± 0.038	3.807ProVal: 3.807 ± 0.054	0.731ProTrp: 0.731 ± 0.024	1.203ProTyr: 1.203 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	6.526GlnAla: 6.526 ± 0.098	0.356GlnCys: 0.356 ± 0.016	1.895GlnAsp: 1.895 ± 0.032	1.928GlnGlu: 1.928 ± 0.041	1.4GlnPhe: 1.4 ± 0.028	3.773GlnGly: 3.773 ± 0.05	1.258GlnHis: 1.258 ± 0.031	2.19GlnIle: 2.19 ± 0.042	1.242GlnLys: 1.242 ± 0.031	6.092GlnLeu: 6.092 ± 0.085	1.14GlnMet: 1.14 ± 0.031	1.085GlnAsn: 1.085 ± 0.025	2.476GlnPro: 2.476 ± 0.047	3.044GlnGln: 3.044 ± 0.056	3.934GlnArg: 3.934 ± 0.062	2.427GlnSer: 2.427 ± 0.036	2.139GlnThr: 2.139 ± 0.037	4.054GlnVal: 4.054 ± 0.059	0.784GlnTrp: 0.784 ± 0.025	1.062GlnTyr: 1.062 ± 0.027	0.0GlnXaa: 0.0 ± 0.0
Arg	5.789ArgAla: 5.789 ± 0.072	0.614ArgCys: 0.614 ± 0.02	3.398ArgAsp: 3.398 ± 0.048	4.097ArgGlu: 4.097 ± 0.054	2.671ArgPhe: 2.671 ± 0.04	3.989ArgGly: 3.989 ± 0.055	1.676ArgHis: 1.676 ± 0.033	3.226ArgIle: 3.226 ± 0.046	2.082ArgLys: 2.082 ± 0.037	8.541ArgLeu: 8.541 ± 0.099	1.621ArgMet: 1.621 ± 0.032	2.023ArgAsn: 2.023 ± 0.035	2.655ArgPro: 2.655 ± 0.043	3.544ArgGln: 3.544 ± 0.056	4.172ArgArg: 4.172 ± 0.064	3.547ArgSer: 3.547 ± 0.052	2.679ArgThr: 2.679 ± 0.037	4.311ArgVal: 4.311 ± 0.055	1.078ArgTrp: 1.078 ± 0.028	2.066ArgTyr: 2.066 ± 0.04	0.0ArgXaa: 0.0 ± 0.0
Ser	6.288SerAla: 6.288 ± 0.066	0.502SerCys: 0.502 ± 0.018	3.001SerAsp: 3.001 ± 0.052	3.152SerGlu: 3.152 ± 0.042	2.177SerPhe: 2.177 ± 0.042	5.128SerGly: 5.128 ± 0.067	1.335SerHis: 1.335 ± 0.031	2.74SerIle: 2.74 ± 0.043	1.893SerLys: 1.893 ± 0.037	7.22SerLeu: 7.22 ± 0.077	1.38SerMet: 1.38 ± 0.029	1.874SerAsn: 1.874 ± 0.04	2.658SerPro: 2.658 ± 0.042	2.627SerGln: 2.627 ± 0.045	3.657SerArg: 3.657 ± 0.051	3.603SerSer: 3.603 ± 0.05	2.967SerThr: 2.967 ± 0.043	3.967SerVal: 3.967 ± 0.047	0.836SerTrp: 0.836 ± 0.025	1.531SerTyr: 1.531 ± 0.034	0.001SerXaa: 0.001 ± 0.001
Thr	5.012ThrAla: 5.012 ± 0.057	0.506ThrCys: 0.506 ± 0.02	2.357ThrAsp: 2.357 ± 0.042	2.326ThrGlu: 2.326 ± 0.035	1.791ThrPhe: 1.791 ± 0.033	3.949ThrGly: 3.949 ± 0.057	1.041ThrHis: 1.041 ± 0.025	1.901ThrIle: 1.901 ± 0.044	1.1ThrLys: 1.1 ± 0.029	6.822ThrLeu: 6.822 ± 0.061	0.798ThrMet: 0.798 ± 0.025	1.19ThrAsn: 1.19 ± 0.032	3.035ThrPro: 3.035 ± 0.05	2.023ThrGln: 2.023 ± 0.035	3.044ThrArg: 3.044 ± 0.042	2.607ThrSer: 2.607 ± 0.05	2.35ThrThr: 2.35 ± 0.042	3.594ThrVal: 3.594 ± 0.054	0.696ThrTrp: 0.696 ± 0.021	1.258ThrTyr: 1.258 ± 0.036	0.0ThrXaa: 0.0 ± 0.0
Val	7.752ValAla: 7.752 ± 0.075	0.774ValCys: 0.774 ± 0.022	3.985ValAsp: 3.985 ± 0.05	4.438ValGlu: 4.438 ± 0.063	2.572ValPhe: 2.572 ± 0.047	5.234ValGly: 5.234 ± 0.062	1.48ValHis: 1.48 ± 0.03	3.865ValIle: 3.865 ± 0.056	2.427ValLys: 2.427 ± 0.044	8.442ValLeu: 8.442 ± 0.078	1.734ValMet: 1.734 ± 0.036	2.266ValAsn: 2.266 ± 0.047	3.34ValPro: 3.34 ± 0.043	2.951ValGln: 2.951 ± 0.044	4.249ValArg: 4.249 ± 0.053	4.307ValSer: 4.307 ± 0.063	3.524ValThr: 3.524 ± 0.056	5.36ValVal: 5.36 ± 0.077	0.86ValTrp: 0.86 ± 0.023	1.626ValTyr: 1.626 ± 0.034	0.0ValXaa: 0.0 ± 0.0
Trp	1.243TrpAla: 1.243 ± 0.033	0.173TrpCys: 0.173 ± 0.011	0.585TrpAsp: 0.585 ± 0.02	0.584TrpGlu: 0.584 ± 0.018	0.568TrpPhe: 0.568 ± 0.023	0.918TrpGly: 0.918 ± 0.025	0.388TrpHis: 0.388 ± 0.017	0.677TrpIle: 0.677 ± 0.02	0.461TrpLys: 0.461 ± 0.018	2.244TrpLeu: 2.244 ± 0.045	0.394TrpMet: 0.394 ± 0.016	0.464TrpAsn: 0.464 ± 0.018	0.666TrpPro: 0.666 ± 0.021	0.996TrpGln: 0.996 ± 0.026	1.101TrpArg: 1.101 ± 0.028	0.83TrpSer: 0.83 ± 0.021	0.609TrpThr: 0.609 ± 0.02	0.949TrpVal: 0.949 ± 0.026	0.248TrpTrp: 0.248 ± 0.013	0.352TrpTyr: 0.352 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.574TyrAla: 2.574 ± 0.041	0.305TyrCys: 0.305 ± 0.015	1.35TyrAsp: 1.35 ± 0.03	1.221TyrGlu: 1.221 ± 0.03	1.03TyrPhe: 1.03 ± 0.03	2.05TyrGly: 2.05 ± 0.042	0.591TyrHis: 0.591 ± 0.02	1.02TyrIle: 1.02 ± 0.021	0.796TyrLys: 0.796 ± 0.023	3.189TyrLeu: 3.189 ± 0.048	0.501TyrMet: 0.501 ± 0.02	0.776TyrAsn: 0.776 ± 0.024	1.312TyrPro: 1.312 ± 0.029	1.378TyrGln: 1.378 ± 0.028	1.997TyrArg: 1.997 ± 0.036	1.631TyrSer: 1.631 ± 0.034	1.239TyrThr: 1.239 ± 0.03	1.591TyrVal: 1.591 ± 0.028	0.442TyrTrp: 0.442 ± 0.017	0.754TyrTyr: 0.754 ± 0.026	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.005XaaXaa: 0.005 ± 0.004

Statistics based on 4863 proteins (1611599 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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