Flavobacterium granuli

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae;

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3250 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M5R229|A0A1M5R229_9FLAO Uncharacterized protein OS=Flavobacterium granuli OX=280093 GN=SAMN05443373_10911 PE=4 SV=1
MM1 pKa = 7.41EE2 pKa = 5.11VICKK6 pKa = 10.03EE7 pKa = 3.79EE8 pKa = 3.6QLRR11 pKa = 11.84FVGGSGGYY19 pKa = 7.22TWQQFVDD26 pKa = 3.93AVNSGNLSSIPAGSYY41 pKa = 10.44IMSSSGDD48 pKa = 3.42SFSFFGEE55 pKa = 4.05GLNEE59 pKa = 4.23VIVLDD64 pKa = 3.78KK65 pKa = 10.84TPAYY69 pKa = 10.11SSSGYY74 pKa = 10.48SSWSWTNTTTGGSGSFFLDD93 pKa = 3.47SIGYY97 pKa = 9.33SDD99 pKa = 5.23FYY101 pKa = 11.5NGGSGSTSNGGDD113 pKa = 3.45SPVDD117 pKa = 3.44SFVNSMLSLVDD128 pKa = 4.0SSDD131 pKa = 3.63DD132 pKa = 3.45NPYY135 pKa = 10.97RR136 pKa = 11.84NWTLEE141 pKa = 3.76TVANVFHH148 pKa = 7.0GMGDD152 pKa = 3.54SVNDD156 pKa = 3.54FGIVLNATGIGAEE169 pKa = 4.08VGVPLMTFGGTISLIGTGIDD189 pKa = 3.34FFIDD193 pKa = 3.3LRR195 pKa = 11.84DD196 pKa = 3.6NTIQSFDD203 pKa = 3.52YY204 pKa = 10.28AAWAITVGLEE214 pKa = 3.72ANSIVFGKK222 pKa = 8.6FTTTTGEE229 pKa = 3.99KK230 pKa = 9.73IIFEE234 pKa = 4.4LSQNGIEE241 pKa = 4.45YY242 pKa = 10.22IKK244 pKa = 10.61EE245 pKa = 3.81

Molecular weight: 26.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M5STD7|A0A1M5STD7_9FLAO Type IX secretion system membrane protein PorP/SprF family OS=Flavobacterium granuli OX=280093 GN=SAMN05443373_112132 PE=4 SV=1
MM1 pKa = 7.54SVRR4 pKa = 11.84KK5 pKa = 9.68LKK7 pKa = 10.5PITPGQRR14 pKa = 11.84FRR16 pKa = 11.84VVNGYY21 pKa = 9.53DD22 pKa = 4.32AITTDD27 pKa = 3.14KK28 pKa = 10.95PEE30 pKa = 4.34RR31 pKa = 11.84SLIAPIKK38 pKa = 10.47NSGGRR43 pKa = 11.84NSQGKK48 pKa = 6.33MTMRR52 pKa = 11.84YY53 pKa = 7.39TGGGHH58 pKa = 4.86KK59 pKa = 9.44QRR61 pKa = 11.84YY62 pKa = 8.74RR63 pKa = 11.84IIDD66 pKa = 3.81FKK68 pKa = 10.3RR69 pKa = 11.84TKK71 pKa = 10.51DD72 pKa = 3.79GIPATVKK79 pKa = 10.64SIEE82 pKa = 4.0YY83 pKa = 10.15DD84 pKa = 3.43PNRR87 pKa = 11.84TAFIALLAYY96 pKa = 10.31ADD98 pKa = 3.92GEE100 pKa = 4.34KK101 pKa = 10.14TYY103 pKa = 11.26VIAQNGLKK111 pKa = 10.23VGQKK115 pKa = 9.86LVSGPEE121 pKa = 3.92SQPEE125 pKa = 3.92IGNTLPLSRR134 pKa = 11.84IPLGTVISCIEE145 pKa = 3.9LRR147 pKa = 11.84PGQGAVIARR156 pKa = 11.84SAGTFAQLMARR167 pKa = 11.84DD168 pKa = 4.06GKK170 pKa = 10.75YY171 pKa = 9.05ATIKK175 pKa = 9.6MPSGEE180 pKa = 4.18TRR182 pKa = 11.84LILLTCSATIGAVSNSDD199 pKa = 3.15HH200 pKa = 6.2QLVVSGKK207 pKa = 9.95AGRR210 pKa = 11.84TRR212 pKa = 11.84WLGRR216 pKa = 11.84RR217 pKa = 11.84PRR219 pKa = 11.84TRR221 pKa = 11.84PVAMNPVDD229 pKa = 3.63HH230 pKa = 7.13PMGGGEE236 pKa = 4.08GRR238 pKa = 11.84SSGGHH243 pKa = 4.7PRR245 pKa = 11.84SRR247 pKa = 11.84NGIPAKK253 pKa = 10.29GYY255 pKa = 7.29RR256 pKa = 11.84TRR258 pKa = 11.84SKK260 pKa = 10.85KK261 pKa = 10.4NPSNKK266 pKa = 10.11YY267 pKa = 8.41IVEE270 pKa = 4.07RR271 pKa = 11.84RR272 pKa = 11.84KK273 pKa = 10.24KK274 pKa = 10.1

Molecular weight: 29.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3250

0

3250

1109611

30

4224

341.4

38.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.7 ± 0.041

0.782 ± 0.016

5.281 ± 0.033

6.387 ± 0.043

5.248 ± 0.032

6.29 ± 0.044

1.679 ± 0.018

8.09 ± 0.04

8.077 ± 0.049

9.259 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.255 ± 0.025

6.256 ± 0.042

3.373 ± 0.024

3.514 ± 0.026

3.238 ± 0.028

6.638 ± 0.037

5.811 ± 0.062

6.075 ± 0.038

1.021 ± 0.018

4.025 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski