Gordonia phage Mahdia

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Gustavvirus; Gordonia virus Mahdia

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H4PAE0|A0A2H4PAE0_9CAUD Uncharacterized protein OS=Gordonia phage Mahdia OX=2047873 GN=PBI_MAHDIA_50 PE=4 SV=1
MM1 pKa = 7.55KK2 pKa = 10.33NYY4 pKa = 8.24TKK6 pKa = 10.9LITSLAALVAVVGIWGGLLAACASEE31 pKa = 4.39SSTASSTPTQTTVTPSPNVLPSEE54 pKa = 4.37TTDD57 pKa = 3.47TEE59 pKa = 4.2EE60 pKa = 4.21TAVTEE65 pKa = 4.23YY66 pKa = 10.77RR67 pKa = 11.84DD68 pKa = 3.56PADD71 pKa = 3.44VSEE74 pKa = 5.33LPLEE78 pKa = 4.37VQVYY82 pKa = 8.45TSMAADD88 pKa = 3.69LVDD91 pKa = 4.9AGTLPSSAVTISAVQADD108 pKa = 3.97VARR111 pKa = 11.84EE112 pKa = 3.77YY113 pKa = 11.24GITLTAAEE121 pKa = 4.95ADD123 pKa = 3.95TVVQAILRR131 pKa = 4.1

Molecular weight: 13.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H4PAA5|A0A2H4PAA5_9CAUD Uncharacterized protein OS=Gordonia phage Mahdia OX=2047873 GN=PBI_MAHDIA_10 PE=4 SV=1
MM1 pKa = 7.03NQHH4 pKa = 7.03DD5 pKa = 4.31DD6 pKa = 3.51EE7 pKa = 4.78PRR9 pKa = 11.84RR10 pKa = 11.84SSGGYY15 pKa = 8.54VPFMPGQGPRR25 pKa = 11.84PNRR28 pKa = 11.84AQRR31 pKa = 11.84RR32 pKa = 11.84AKK34 pKa = 10.38DD35 pKa = 3.37QIRR38 pKa = 11.84ARR40 pKa = 11.84GRR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84MEE46 pKa = 3.9RR47 pKa = 11.84ATVRR51 pKa = 11.84ALKK54 pKa = 9.69QAQRR58 pKa = 11.84QDD60 pKa = 3.52LRR62 pKa = 11.84PQLSEE67 pKa = 4.33DD68 pKa = 3.37MAEE71 pKa = 4.19GSVTVSVDD79 pKa = 3.55LEE81 pKa = 4.39EE82 pKa = 4.59GTVTEE87 pKa = 4.54TGG89 pKa = 3.23

Molecular weight: 10.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

61

0

61

13558

61

1416

222.3

24.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.713 ± 0.421

0.509 ± 0.093

6.439 ± 0.317

6.203 ± 0.252

2.368 ± 0.155

9.035 ± 0.487

1.822 ± 0.17

3.909 ± 0.191

3.585 ± 0.209

8.497 ± 0.24

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.235 ± 0.103

2.81 ± 0.26

5.945 ± 0.299

3.371 ± 0.176

6.719 ± 0.359

5.495 ± 0.23

7.25 ± 0.3

7.582 ± 0.218

2.043 ± 0.147

2.471 ± 0.165
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski