Aeromicrobium sp. 592
Average proteome isoelectric point is 5.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3511 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S0WRQ6|A0A2S0WRQ6_9ACTN Deoxyribodipyrimidine photolyase OS=Aeromicrobium sp. 592 OX=2079793 GN=C3E78_03485 PE=3 SV=1
MM1 pKa = 6.96 TTTDD5 pKa = 3.4 TYY7 pKa = 11.12 RR8 pKa = 11.84 VTGMTCEE15 pKa = 4.03 HH16 pKa = 6.71 CVAAVTEE23 pKa = 4.35 EE24 pKa = 4.43 LSALDD29 pKa = 4.47 DD30 pKa = 3.97 VTSVAVDD37 pKa = 3.78 LVPGGTSTVTVEE49 pKa = 4.34 SSAPLDD55 pKa = 3.71 PATVAEE61 pKa = 4.58 AVDD64 pKa = 3.51 EE65 pKa = 4.59 AGYY68 pKa = 11.28 ALAGPRR74 pKa = 11.84 DD75 pKa = 3.75 LPLAA79 pKa = 4.34
Molecular weight: 8.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.788
IPC2_protein 3.681
IPC_protein 3.579
Toseland 3.401
ProMoST 3.783
Dawson 3.592
Bjellqvist 3.757
Wikipedia 3.554
Rodwell 3.427
Grimsley 3.325
Solomon 3.554
Lehninger 3.503
Nozaki 3.732
DTASelect 3.897
Thurlkill 3.478
EMBOSS 3.554
Sillero 3.719
Patrickios 0.604
IPC_peptide 3.541
IPC2_peptide 3.681
IPC2.peptide.svr19 3.691
Protein with the highest isoelectric point:
>tr|A0A2S0WJE5|A0A2S0WJE5_9ACTN Uncharacterized protein OS=Aeromicrobium sp. 592 OX=2079793 GN=C3E78_03805 PE=4 SV=1
MM1 pKa = 6.58 TTPPRR6 pKa = 11.84 TSPSRR11 pKa = 11.84 RR12 pKa = 11.84 PAHH15 pKa = 5.96 SAASSRR21 pKa = 11.84 SRR23 pKa = 11.84 SPRR26 pKa = 11.84 PHH28 pKa = 6.29 LRR30 pKa = 11.84 PRR32 pKa = 11.84 LRR34 pKa = 11.84 GRR36 pKa = 11.84 PPTRR40 pKa = 11.84 RR41 pKa = 11.84 PPRR44 pKa = 11.84 LQPPRR49 pKa = 11.84 LRR51 pKa = 11.84 PPRR54 pKa = 11.84 LHH56 pKa = 6.4 RR57 pKa = 11.84 PRR59 pKa = 11.84 RR60 pKa = 11.84 RR61 pKa = 11.84 RR62 pKa = 11.84 PSAPSTDD69 pKa = 3.71 RR70 pKa = 11.84 PPGSPHH76 pKa = 6.37 AAAVAPSS83 pKa = 3.21
Molecular weight: 9.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.52
IPC2_protein 11.228
IPC_protein 12.837
Toseland 12.998
ProMoST 13.495
Dawson 12.998
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.501
Grimsley 13.042
Solomon 13.495
Lehninger 13.393
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.495
Sillero 12.998
Patrickios 12.223
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.217
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3511
0
3511
1133916
29
3019
323.0
34.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.648 ± 0.059
0.636 ± 0.01
6.6 ± 0.033
5.569 ± 0.044
2.927 ± 0.022
9.012 ± 0.039
2.162 ± 0.023
4.122 ± 0.03
2.352 ± 0.035
10.032 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.933 ± 0.017
1.851 ± 0.021
5.287 ± 0.028
2.834 ± 0.02
7.345 ± 0.041
5.663 ± 0.033
6.249 ± 0.037
9.368 ± 0.037
1.435 ± 0.017
1.976 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here