Streptococcus phage Javan135
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6A096|A0A4D6A096_9CAUD Uncharacterized protein OS=Streptococcus phage Javan135 OX=2547998 GN=Javan135_0046 PE=4 SV=1
MM1 pKa = 7.52 NYY3 pKa = 9.5 IEE5 pKa = 4.55 YY6 pKa = 10.82 ALAYY10 pKa = 10.17 LEE12 pKa = 4.77 RR13 pKa = 11.84 EE14 pKa = 4.22 LEE16 pKa = 4.5 IIDD19 pKa = 3.9 NEE21 pKa = 4.38 VIEE24 pKa = 4.64 VEE26 pKa = 4.78 LPDD29 pKa = 4.41 GDD31 pKa = 3.85 WEE33 pKa = 4.53 FVLNPYY39 pKa = 9.86 YY40 pKa = 10.47 EE41 pKa = 4.81 EE42 pKa = 4.72 GLHH45 pKa = 6.64 DD46 pKa = 3.79 SPYY49 pKa = 10.48 YY50 pKa = 10.02 RR51 pKa = 11.84 SQVAKK56 pKa = 10.55 DD57 pKa = 3.09 ILDD60 pKa = 3.46 IKK62 pKa = 10.8 GLLGRR67 pKa = 4.72
Molecular weight: 7.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.847
IPC2_protein 4.101
IPC_protein 3.973
Toseland 3.808
ProMoST 4.113
Dawson 3.935
Bjellqvist 4.088
Wikipedia 3.834
Rodwell 3.821
Grimsley 3.719
Solomon 3.923
Lehninger 3.872
Nozaki 4.062
DTASelect 4.19
Thurlkill 3.846
EMBOSS 3.846
Sillero 4.088
Patrickios 0.846
IPC_peptide 3.923
IPC2_peptide 4.075
IPC2.peptide.svr19 3.977
Protein with the highest isoelectric point:
>tr|A0A4D6A4D3|A0A4D6A4D3_9CAUD Hyaluronidase OS=Streptococcus phage Javan135 OX=2547998 GN=Javan135_0045 PE=4 SV=1
MM1 pKa = 7.9 RR2 pKa = 11.84 GLDD5 pKa = 3.16 KK6 pKa = 10.66 FLRR9 pKa = 11.84 SVEE12 pKa = 4.13 RR13 pKa = 11.84 KK14 pKa = 8.65 QKK16 pKa = 9.38 SVRR19 pKa = 11.84 IAVDD23 pKa = 3.31 KK24 pKa = 10.73 EE25 pKa = 4.09 LSKK28 pKa = 10.9 SAARR32 pKa = 11.84 IEE34 pKa = 4.15 RR35 pKa = 11.84 QAKK38 pKa = 9.19 ILAPVDD44 pKa = 3.94 TGWLRR49 pKa = 11.84 AQIYY53 pKa = 10.31 NEE55 pKa = 3.95 QQRR58 pKa = 11.84 LLHH61 pKa = 5.57 YY62 pKa = 9.97 RR63 pKa = 11.84 VVSPALYY70 pKa = 10.27 SIYY73 pKa = 10.79 LEE75 pKa = 4.28 LGTRR79 pKa = 11.84 KK80 pKa = 8.87 MEE82 pKa = 4.32 AQPFLDD88 pKa = 3.7 PALRR92 pKa = 11.84 KK93 pKa = 8.48 EE94 pKa = 4.46 WPVLMANLKK103 pKa = 10.77 KK104 pKa = 9.96 MFKK107 pKa = 10.29 RR108 pKa = 3.74
Molecular weight: 12.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.271
IPC2_protein 9.706
IPC_protein 10.145
Toseland 10.657
ProMoST 10.379
Dawson 10.76
Bjellqvist 10.394
Wikipedia 10.906
Rodwell 11.169
Grimsley 10.804
Solomon 10.818
Lehninger 10.804
Nozaki 10.613
DTASelect 10.394
Thurlkill 10.643
EMBOSS 11.038
Sillero 10.672
Patrickios 10.906
IPC_peptide 10.833
IPC2_peptide 8.96
IPC2.peptide.svr19 8.754
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
54
0
54
12234
37
1385
226.6
25.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.117 ± 0.738
0.482 ± 0.108
6.122 ± 0.484
7.43 ± 0.687
3.588 ± 0.225
6.539 ± 0.512
1.283 ± 0.158
6.997 ± 0.303
8.599 ± 0.601
8.754 ± 0.359
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.387 ± 0.179
5.019 ± 0.308
2.804 ± 0.242
4.013 ± 0.312
4.299 ± 0.385
6.694 ± 0.81
5.885 ± 0.319
6.122 ± 0.238
1.103 ± 0.124
3.76 ± 0.451
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here