Streptococcus phage Javan135

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4D6A096|A0A4D6A096_9CAUD Uncharacterized protein OS=Streptococcus phage Javan135 OX=2547998 GN=Javan135_0046 PE=4 SV=1
MM1 pKa = 7.52NYY3 pKa = 9.5IEE5 pKa = 4.55YY6 pKa = 10.82ALAYY10 pKa = 10.17LEE12 pKa = 4.77RR13 pKa = 11.84EE14 pKa = 4.22LEE16 pKa = 4.5IIDD19 pKa = 3.9NEE21 pKa = 4.38VIEE24 pKa = 4.64VEE26 pKa = 4.78LPDD29 pKa = 4.41GDD31 pKa = 3.85WEE33 pKa = 4.53FVLNPYY39 pKa = 9.86YY40 pKa = 10.47EE41 pKa = 4.81EE42 pKa = 4.72GLHH45 pKa = 6.64DD46 pKa = 3.79SPYY49 pKa = 10.48YY50 pKa = 10.02RR51 pKa = 11.84SQVAKK56 pKa = 10.55DD57 pKa = 3.09ILDD60 pKa = 3.46IKK62 pKa = 10.8GLLGRR67 pKa = 4.72

Molecular weight:
7.94 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4D6A4D3|A0A4D6A4D3_9CAUD Hyaluronidase OS=Streptococcus phage Javan135 OX=2547998 GN=Javan135_0045 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84GLDD5 pKa = 3.16KK6 pKa = 10.66FLRR9 pKa = 11.84SVEE12 pKa = 4.13RR13 pKa = 11.84KK14 pKa = 8.65QKK16 pKa = 9.38SVRR19 pKa = 11.84IAVDD23 pKa = 3.31KK24 pKa = 10.73EE25 pKa = 4.09LSKK28 pKa = 10.9SAARR32 pKa = 11.84IEE34 pKa = 4.15RR35 pKa = 11.84QAKK38 pKa = 9.19ILAPVDD44 pKa = 3.94TGWLRR49 pKa = 11.84AQIYY53 pKa = 10.31NEE55 pKa = 3.95QQRR58 pKa = 11.84LLHH61 pKa = 5.57YY62 pKa = 9.97RR63 pKa = 11.84VVSPALYY70 pKa = 10.27SIYY73 pKa = 10.79LEE75 pKa = 4.28LGTRR79 pKa = 11.84KK80 pKa = 8.87MEE82 pKa = 4.32AQPFLDD88 pKa = 3.7PALRR92 pKa = 11.84KK93 pKa = 8.48EE94 pKa = 4.46WPVLMANLKK103 pKa = 10.77KK104 pKa = 9.96MFKK107 pKa = 10.29RR108 pKa = 3.74

Molecular weight:
12.73 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

12234

37

1385

226.6

25.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.117 ± 0.738

0.482 ± 0.108

6.122 ± 0.484

7.43 ± 0.687

3.588 ± 0.225

6.539 ± 0.512

1.283 ± 0.158

6.997 ± 0.303

8.599 ± 0.601

8.754 ± 0.359

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.387 ± 0.179

5.019 ± 0.308

2.804 ± 0.242

4.013 ± 0.312

4.299 ± 0.385

6.694 ± 0.81

5.885 ± 0.319

6.122 ± 0.238

1.103 ± 0.124

3.76 ± 0.451

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski