Rubus yellow net virus

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Caulimoviridae; Badnavirus

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A097DCZ5|A0A097DCZ5_9VIRU Reverse transcriptase OS=Rubus yellow net virus OX=198310 PE=4 SV=1
MM1 pKa = 7.46CCALGVFCCYY11 pKa = 8.29QHH13 pKa = 7.27DD14 pKa = 5.04PIVHH18 pKa = 6.49PCTYY22 pKa = 9.19RR23 pKa = 11.84TEE25 pKa = 4.11VDD27 pKa = 4.01VACSAGDD34 pKa = 3.58SSTVRR39 pKa = 11.84CFVTTDD45 pKa = 3.08AANPVADD52 pKa = 3.71RR53 pKa = 11.84HH54 pKa = 5.65FLGVTPGVCAVLLDD68 pKa = 3.94LL69 pKa = 5.99

Molecular weight: 7.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A097DCX2|A0A097DCX2_9VIRU Uncharacterized protein OS=Rubus yellow net virus OX=198310 PE=4 SV=1
MM1 pKa = 7.28NPRR4 pKa = 11.84WQEE7 pKa = 3.56TAAKK11 pKa = 7.26EE12 pKa = 4.27TYY14 pKa = 9.77IKK16 pKa = 10.42AIQATASLTSNGTGQGFIEE35 pKa = 4.13PHH37 pKa = 6.65TYY39 pKa = 9.77TGGQLSTNLAKK50 pKa = 10.64QNNTIIEE57 pKa = 4.33LLVQVLEE64 pKa = 4.47KK65 pKa = 11.14NLDD68 pKa = 3.59LEE70 pKa = 4.25RR71 pKa = 11.84AVANLTGQVTRR82 pKa = 11.84LEE84 pKa = 4.1KK85 pKa = 10.68AVADD89 pKa = 3.98KK90 pKa = 10.98EE91 pKa = 4.39AVKK94 pKa = 10.76LPEE97 pKa = 4.79KK98 pKa = 10.66VLEE101 pKa = 4.57DD102 pKa = 3.34LTKK105 pKa = 10.94EE106 pKa = 3.94FGKK109 pKa = 11.12VNLGKK114 pKa = 11.0GKK116 pKa = 9.76GKK118 pKa = 9.75KK119 pKa = 9.5RR120 pKa = 11.84KK121 pKa = 9.06VSSRR125 pKa = 11.84DD126 pKa = 3.23RR127 pKa = 11.84TSTCGKK133 pKa = 8.59PLHH136 pKa = 6.76SIQQ139 pKa = 4.36

Molecular weight: 15.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2532

69

1976

506.4

57.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.872 ± 1.962

1.777 ± 1.284

5.648 ± 0.553

7.978 ± 0.875

2.883 ± 0.302

5.885 ± 0.966

2.133 ± 0.375

5.45 ± 0.713

6.556 ± 1.429

8.017 ± 1.134

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.567 ± 0.7

3.752 ± 0.434

5.569 ± 0.46

4.621 ± 0.751

6.438 ± 1.168

6.714 ± 0.46

6.872 ± 0.744

5.332 ± 1.221

1.659 ± 0.426

3.278 ± 0.487
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski