Chimpanzee associated porprismacovirus 2
Average proteome isoelectric point is 7.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D2WKE9|D2WKE9_9VIRU Putative replicase protein OS=Chimpanzee associated porprismacovirus 2 OX=2170110 GN=rep PE=4 SV=1
MM1 pKa = 7.45 HH2 pKa = 7.8 LFTEE6 pKa = 4.83 VVSVDD11 pKa = 3.23 IMTTMQRR18 pKa = 11.84 THH20 pKa = 6.13 TNAEE24 pKa = 4.22 LIRR27 pKa = 11.84 WFKK30 pKa = 10.53 IFRR33 pKa = 11.84 DD34 pKa = 3.64 LDD36 pKa = 3.17 IHH38 pKa = 6.15 KK39 pKa = 9.68 WVIGLEE45 pKa = 4.11 EE46 pKa = 4.29 GKK48 pKa = 10.53 GGYY51 pKa = 7.67 GHH53 pKa = 6.29 WQVRR57 pKa = 11.84 CNIRR61 pKa = 11.84 VEE63 pKa = 4.38 VVNDD67 pKa = 3.09 WTAYY71 pKa = 9.53 LRR73 pKa = 11.84 AVFSWLGPISIWTEE87 pKa = 3.84 EE88 pKa = 4.25 CSDD91 pKa = 3.29 KK92 pKa = 10.49 YY93 pKa = 9.64 TYY95 pKa = 7.33 EE96 pKa = 4.28 TKK98 pKa = 10.41 EE99 pKa = 3.76 GRR101 pKa = 11.84 YY102 pKa = 6.08 WASWDD107 pKa = 3.46 TMGARR112 pKa = 11.84 QQRR115 pKa = 11.84 FGKK118 pKa = 9.08 MRR120 pKa = 11.84 WNQEE124 pKa = 3.52 GAVQALQRR132 pKa = 11.84 TNDD135 pKa = 3.34 RR136 pKa = 11.84 EE137 pKa = 4.07 IVVWYY142 pKa = 9.93 DD143 pKa = 3.11 EE144 pKa = 4.06 QGNMGKK150 pKa = 9.22 SWLCGHH156 pKa = 7.23 LFEE159 pKa = 5.62 TGQAYY164 pKa = 8.57 YY165 pKa = 9.68 IPPYY169 pKa = 7.6 MTSIQSMIQTVASLVLQDD187 pKa = 4.59 RR188 pKa = 11.84 DD189 pKa = 3.05 SGYY192 pKa = 9.92 PPRR195 pKa = 11.84 PLIVIDD201 pKa = 5.23 IPRR204 pKa = 11.84 SWKK207 pKa = 9.63 WSTEE211 pKa = 3.64 LYY213 pKa = 9.64 TAIEE217 pKa = 4.54 AIKK220 pKa = 10.41 DD221 pKa = 3.74 GLIMDD226 pKa = 4.67 PRR228 pKa = 11.84 YY229 pKa = 9.71 GARR232 pKa = 11.84 PVNIHH237 pKa = 6.31 GIKK240 pKa = 10.53 VIVLTNTKK248 pKa = 9.98 PKK250 pKa = 10.2 LDD252 pKa = 3.97 KK253 pKa = 10.88 LSEE256 pKa = 4.3 DD257 pKa = 2.82 RR258 pKa = 11.84 WVLYY262 pKa = 10.96 DD263 pKa = 3.65 PMDD266 pKa = 3.94 YY267 pKa = 11.48 PMMLL271 pKa = 3.63
Molecular weight: 31.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.972
IPC2_protein 5.97
IPC_protein 6.059
Toseland 6.427
ProMoST 6.415
Dawson 6.351
Bjellqvist 6.326
Wikipedia 6.364
Rodwell 6.338
Grimsley 6.576
Solomon 6.351
Lehninger 6.351
Nozaki 6.605
DTASelect 6.795
Thurlkill 6.81
EMBOSS 6.795
Sillero 6.722
Patrickios 4.101
IPC_peptide 6.376
IPC2_peptide 6.634
IPC2.peptide.svr19 6.641
Protein with the highest isoelectric point:
>tr|D2WKE5|D2WKE5_9VIRU ORF3 OS=Chimpanzee associated porprismacovirus 2 OX=2170110 PE=4 SV=1
MM1 pKa = 7.83 SIRR4 pKa = 11.84 IKK6 pKa = 10.46 IPQVLIKK13 pKa = 10.39 GCPRR17 pKa = 11.84 RR18 pKa = 11.84 PLEE21 pKa = 3.62 YY22 pKa = 10.61 AFVYY26 pKa = 10.45 GSGISGHH33 pKa = 6.23 NDD35 pKa = 2.67 NNAKK39 pKa = 10.21 DD40 pKa = 3.62 AYY42 pKa = 9.37 QRR44 pKa = 11.84 RR45 pKa = 11.84 ANPLVQNLPRR55 pKa = 11.84 SRR57 pKa = 11.84 YY58 pKa = 7.66 PQMGHH63 pKa = 6.47 RR64 pKa = 11.84 AGGRR68 pKa = 11.84 QRR70 pKa = 11.84 RR71 pKa = 11.84 IRR73 pKa = 11.84 TLAGQMQYY81 pKa = 10.62 PGRR84 pKa = 11.84 SGQRR88 pKa = 11.84 LDD90 pKa = 4.67 SIPKK94 pKa = 10.25 GSILMARR101 pKa = 11.84 TNLDD105 pKa = 3.03 LDD107 pKa = 3.39 RR108 pKa = 11.84 GMFRR112 pKa = 11.84 QVHH115 pKa = 5.86 LRR117 pKa = 11.84 DD118 pKa = 3.15 QGRR121 pKa = 11.84 QVLGIMGYY129 pKa = 10.48 DD130 pKa = 3.52 GSEE133 pKa = 3.87 TTAVRR138 pKa = 11.84 EE139 pKa = 4.08 NEE141 pKa = 3.85 MEE143 pKa = 4.01 PRR145 pKa = 11.84 RR146 pKa = 11.84 GRR148 pKa = 11.84 ASPTTDD154 pKa = 2.75 EE155 pKa = 4.55 RR156 pKa = 11.84 PRR158 pKa = 11.84 DD159 pKa = 3.59 SGLVRR164 pKa = 11.84 RR165 pKa = 11.84 TRR167 pKa = 11.84 QHH169 pKa = 4.86 GQIVALRR176 pKa = 11.84 APIRR180 pKa = 11.84 DD181 pKa = 3.48 RR182 pKa = 11.84 AGLLHH187 pKa = 6.06 TAVHH191 pKa = 6.94 DD192 pKa = 3.6 EE193 pKa = 4.27 HH194 pKa = 6.69 TKK196 pKa = 10.11 HH197 pKa = 6.79 DD198 pKa = 4.58 SNRR201 pKa = 11.84 SEE203 pKa = 4.19 PSPTRR208 pKa = 11.84 PRR210 pKa = 3.53
Molecular weight: 23.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.185
IPC2_protein 9.999
IPC_protein 11.301
Toseland 11.389
ProMoST 11.871
Dawson 11.403
Bjellqvist 11.374
Wikipedia 11.857
Rodwell 11.125
Grimsley 11.447
Solomon 11.857
Lehninger 11.769
Nozaki 11.374
DTASelect 11.374
Thurlkill 11.389
EMBOSS 11.871
Sillero 11.389
Patrickios 10.833
IPC_peptide 11.857
IPC2_peptide 10.833
IPC2.peptide.svr19 9.429
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1554
105
421
259.0
29.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.505 ± 0.821
1.351 ± 0.52
4.826 ± 0.502
4.054 ± 0.397
2.767 ± 0.408
6.435 ± 1.03
3.99 ± 0.583
7.722 ± 1.724
3.99 ± 0.332
8.43 ± 0.471
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.218 ± 0.556
4.762 ± 0.81
5.47 ± 0.375
4.054 ± 0.685
8.559 ± 1.881
8.366 ± 0.956
6.885 ± 0.395
5.856 ± 0.619
1.609 ± 0.789
3.153 ± 0.935
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here