Henriciella sp. JN25

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomonadales; Hyphomonadaceae; Henriciella

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3647 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A399RRE4|A0A399RRE4_9PROT Uncharacterized protein OS=Henriciella sp. JN25 OX=2305466 GN=D1223_03065 PE=4 SV=1
MM1 pKa = 7.43NIWIKK6 pKa = 7.73TTAVTATALAMAACTQSGYY25 pKa = 7.42TEE27 pKa = 4.01RR28 pKa = 11.84NAAAGAAAGAVAGAVIGNNTGDD50 pKa = 3.35GDD52 pKa = 4.08AAEE55 pKa = 4.69GAAIGAAVGGAAGAAKK71 pKa = 10.38GCTEE75 pKa = 4.95AADD78 pKa = 4.11CDD80 pKa = 4.18TPGVKK85 pKa = 10.01DD86 pKa = 3.76QPDD89 pKa = 3.65EE90 pKa = 4.62KK91 pKa = 11.2DD92 pKa = 3.22SDD94 pKa = 3.63GDD96 pKa = 4.29GYY98 pKa = 11.62VDD100 pKa = 5.37AYY102 pKa = 11.07DD103 pKa = 5.12DD104 pKa = 4.08YY105 pKa = 11.49PYY107 pKa = 11.28DD108 pKa = 3.8PNRR111 pKa = 11.84WW112 pKa = 3.32

Molecular weight: 10.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A399R5I8|A0A399R5I8_9PROT Holdfast attachment protein HfaA OS=Henriciella sp. JN25 OX=2305466 GN=D1223_16585 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84LKK11 pKa = 10.1RR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.65GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.46NGRR28 pKa = 11.84KK29 pKa = 8.96VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.16GRR39 pKa = 11.84KK40 pKa = 6.41TLSAA44 pKa = 4.15

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3647

0

3647

1173922

41

2417

321.9

35.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.879 ± 0.048

0.865 ± 0.014

6.349 ± 0.033

6.504 ± 0.038

3.836 ± 0.029

8.539 ± 0.037

1.907 ± 0.021

5.18 ± 0.027

3.542 ± 0.035

9.602 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.541 ± 0.02

2.713 ± 0.023

5.04 ± 0.027

3.112 ± 0.022

6.615 ± 0.039

5.812 ± 0.027

5.284 ± 0.024

6.902 ± 0.034

1.426 ± 0.016

2.35 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski