Coniochaeta pulveracea
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10012 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A420Y1Z3|A0A420Y1Z3_9PEZI Sec39 domain-containing protein OS=Coniochaeta pulveracea OX=177199 GN=DL546_003458 PE=4 SV=1
MM1 pKa = 7.44 IPAPYY6 pKa = 9.58 SASDD10 pKa = 3.43 TNPWLVAKK18 pKa = 10.17 RR19 pKa = 11.84 LPFADD24 pKa = 5.64 HH25 pKa = 7.28 IICLDD30 pKa = 3.57 ASDD33 pKa = 4.79 KK34 pKa = 10.19 VTAQGTFSDD43 pKa = 4.43 LSTAGGYY50 pKa = 8.43 VASFSLPGADD60 pKa = 2.31 WTYY63 pKa = 10.57 ATNNGSDD70 pKa = 4.09 DD71 pKa = 4.5 DD72 pKa = 5.93 DD73 pKa = 6.0 NITDD77 pKa = 3.71 ADD79 pKa = 3.69 QYY81 pKa = 11.71 GDD83 pKa = 3.58 VLAGNKK89 pKa = 9.13 TSDD92 pKa = 3.86 DD93 pKa = 3.93 MLDD96 pKa = 3.48 ASQTSSSSITSKK108 pKa = 10.94 GSTINLEE115 pKa = 4.16 EE116 pKa = 3.92 VSGSNRR122 pKa = 11.84 QAGDD126 pKa = 3.45 LQIYY130 pKa = 8.76 LYY132 pKa = 10.71 YY133 pKa = 9.3 MRR135 pKa = 11.84 SVGWWTSMTFTVAITAFVFCISFPSKK161 pKa = 10.87
Molecular weight: 17.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.728
IPC2_protein 3.872
IPC_protein 3.884
Toseland 3.643
ProMoST 4.088
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.91
Rodwell 3.706
Grimsley 3.554
Solomon 3.897
Lehninger 3.859
Nozaki 4.024
DTASelect 4.355
Thurlkill 3.719
EMBOSS 3.91
Sillero 4.012
Patrickios 1.952
IPC_peptide 3.884
IPC2_peptide 3.986
IPC2.peptide.svr19 3.904
Protein with the highest isoelectric point:
>tr|A0A420YCU6|A0A420YCU6_9PEZI AP complex subunit sigma OS=Coniochaeta pulveracea OX=177199 GN=VAS2 PE=3 SV=1
MM1 pKa = 7.74 AHH3 pKa = 6.38 RR4 pKa = 11.84 QSTTTTTTTTSTVPSKK20 pKa = 11.02 RR21 pKa = 11.84 NLFGRR26 pKa = 11.84 RR27 pKa = 11.84 DD28 pKa = 3.26 RR29 pKa = 11.84 AAHH32 pKa = 5.83 HH33 pKa = 6.1 RR34 pKa = 11.84 HH35 pKa = 5.66 GKK37 pKa = 10.04 LSLGNRR43 pKa = 11.84 VIGAVKK49 pKa = 10.17 RR50 pKa = 11.84 LRR52 pKa = 11.84 GTVVRR57 pKa = 11.84 RR58 pKa = 11.84 PGEE61 pKa = 4.01 KK62 pKa = 9.43 ATGTQHH68 pKa = 5.6 IHH70 pKa = 4.86 EE71 pKa = 4.45 THH73 pKa = 5.84 SRR75 pKa = 11.84 RR76 pKa = 11.84 SVGRR80 pKa = 11.84 SPRR83 pKa = 11.84 FLQRR87 pKa = 11.84 WRR89 pKa = 11.84 RR90 pKa = 11.84 KK91 pKa = 8.95 RR92 pKa = 11.84 AMGVARR98 pKa = 11.84 HH99 pKa = 5.42 SVGLL103 pKa = 3.74
Molecular weight: 11.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.394
IPC2_protein 10.847
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.223
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.945
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.127
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9534
478
10012
4982711
51
7917
497.7
54.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.909 ± 0.025
1.175 ± 0.01
5.748 ± 0.016
6.205 ± 0.029
3.489 ± 0.018
7.17 ± 0.023
2.417 ± 0.012
4.501 ± 0.014
4.839 ± 0.021
8.691 ± 0.027
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.196 ± 0.009
3.505 ± 0.013
6.271 ± 0.027
4.05 ± 0.022
6.135 ± 0.024
7.958 ± 0.026
6.226 ± 0.021
6.374 ± 0.018
1.417 ± 0.01
2.725 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here