Capybara microvirus Cap1_SP_244
Average proteome isoelectric point is 5.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W5I1|A0A4P8W5I1_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_244 OX=2585418 PE=4 SV=1
MM1 pKa = 8.1 DD2 pKa = 6.07 DD3 pKa = 4.35 KK4 pKa = 11.63 VVLPGNIDD12 pKa = 3.63 SNVNYY17 pKa = 9.13 DD18 pKa = 3.58 ASMMPVSNLSDD29 pKa = 3.08 WYY31 pKa = 10.94 GEE33 pKa = 4.33 SGGEE37 pKa = 4.35 KK38 pKa = 10.13 YY39 pKa = 10.6 LQEE42 pKa = 4.16 ATGSNIDD49 pKa = 4.1 LAVMDD54 pKa = 4.72 DD55 pKa = 4.6 RR56 pKa = 11.84 EE57 pKa = 4.23 FEE59 pKa = 4.15 LWTDD63 pKa = 3.09 NTKK66 pKa = 8.84 WQRR69 pKa = 11.84 SVEE72 pKa = 4.02 DD73 pKa = 3.48 MKK75 pKa = 11.12 RR76 pKa = 11.84 AGINPFQALGSSPSTSVISGNQLDD100 pKa = 4.08 ASGKK104 pKa = 10.35 KK105 pKa = 9.89 LDD107 pKa = 4.3 AFLKK111 pKa = 10.76 FLGTFLKK118 pKa = 11.03 LII120 pKa = 4.32
Molecular weight: 13.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.528
IPC2_protein 4.342
IPC_protein 4.266
Toseland 4.062
ProMoST 4.418
Dawson 4.24
Bjellqvist 4.406
Wikipedia 4.177
Rodwell 4.088
Grimsley 3.973
Solomon 4.24
Lehninger 4.19
Nozaki 4.355
DTASelect 4.596
Thurlkill 4.101
EMBOSS 4.19
Sillero 4.38
Patrickios 4.151
IPC_peptide 4.24
IPC2_peptide 4.355
IPC2.peptide.svr19 4.325
Protein with the highest isoelectric point:
>tr|A0A4V1FVR5|A0A4V1FVR5_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_244 OX=2585418 PE=4 SV=1
MM1 pKa = 7.74 SMNCLKK7 pKa = 10.65 PKK9 pKa = 8.77 MAIWNKK15 pKa = 6.99 EE16 pKa = 3.8 TGVFKK21 pKa = 11.08 LINSDD26 pKa = 3.91 DD27 pKa = 3.99 LEE29 pKa = 5.0 SIYY32 pKa = 11.02 RR33 pKa = 11.84 NSNKK37 pKa = 10.22 NYY39 pKa = 8.3 VPLKK43 pKa = 9.91 IRR45 pKa = 11.84 CGKK48 pKa = 10.32 CEE50 pKa = 3.89 PCNLLKK56 pKa = 11.07 SQDD59 pKa = 2.78 WTKK62 pKa = 11.02 RR63 pKa = 11.84 ILCEE67 pKa = 3.61 MKK69 pKa = 10.13 YY70 pKa = 10.73 FKK72 pKa = 10.78 YY73 pKa = 10.58 SYY75 pKa = 10.24 FITLTYY81 pKa = 11.02 DD82 pKa = 3.07 NYY84 pKa = 11.02 HH85 pKa = 6.5 LRR87 pKa = 11.84 DD88 pKa = 3.28 INTRR92 pKa = 11.84 DD93 pKa = 3.02 IQLFIKK99 pKa = 10.32 KK100 pKa = 9.3 LRR102 pKa = 11.84 KK103 pKa = 10.04 KK104 pKa = 9.9 IDD106 pKa = 3.28 RR107 pKa = 11.84 KK108 pKa = 10.49 LKK110 pKa = 9.42 YY111 pKa = 9.21 ICCGEE116 pKa = 4.16 YY117 pKa = 10.61 GSEE120 pKa = 4.13 TKK122 pKa = 10.32 RR123 pKa = 11.84 PHH125 pKa = 5.82 YY126 pKa = 10.74 HH127 pKa = 6.59 MILLSDD133 pKa = 4.09 YY134 pKa = 10.93 EE135 pKa = 4.35 FVPPEE140 pKa = 3.47 WRR142 pKa = 11.84 YY143 pKa = 10.3 NDD145 pKa = 3.38 NTFVCSEE152 pKa = 4.79 LEE154 pKa = 4.6 KK155 pKa = 10.23 IWQNGNVRR163 pKa = 11.84 ISNDD167 pKa = 3.0 VNEE170 pKa = 4.29 RR171 pKa = 11.84 CIKK174 pKa = 9.44 YY175 pKa = 7.58 TLKK178 pKa = 9.84 YY179 pKa = 7.54 TLKK182 pKa = 10.59 NVGEE186 pKa = 4.52 SKK188 pKa = 10.94 VFLYY192 pKa = 10.45 SKK194 pKa = 10.4 KK195 pKa = 10.07 IGYY198 pKa = 7.34 TWFEE202 pKa = 4.16 EE203 pKa = 4.08 NKK205 pKa = 10.34 EE206 pKa = 4.16 DD207 pKa = 4.09 IKK209 pKa = 11.26 NYY211 pKa = 10.29 CGFYY215 pKa = 10.3 SKK217 pKa = 10.75 NGKK220 pKa = 9.36 FEE222 pKa = 4.14 NPPLYY227 pKa = 10.48 FIRR230 pKa = 11.84 KK231 pKa = 6.94 MKK233 pKa = 10.89 NSDD236 pKa = 3.83 DD237 pKa = 4.16 FNDD240 pKa = 3.77 FLWYY244 pKa = 10.13 DD245 pKa = 3.71 DD246 pKa = 4.08 FNKK249 pKa = 10.0 NRR251 pKa = 11.84 LGDD254 pKa = 3.87 TIIDD258 pKa = 3.94 FNDD261 pKa = 2.75 ILASKK266 pKa = 10.27 FYY268 pKa = 10.75 EE269 pKa = 4.08 EE270 pKa = 4.25 KK271 pKa = 10.4 KK272 pKa = 9.96 KK273 pKa = 11.09 KK274 pKa = 9.38 KK275 pKa = 10.63 GKK277 pKa = 10.64 GILL280 pKa = 3.5
Molecular weight: 33.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.566
IPC2_protein 8.492
IPC_protein 8.331
Toseland 8.902
ProMoST 8.873
Dawson 9.282
Bjellqvist 9.18
Wikipedia 9.428
Rodwell 9.472
Grimsley 9.282
Solomon 9.326
Lehninger 9.297
Nozaki 9.311
DTASelect 9.063
Thurlkill 9.18
EMBOSS 9.414
Sillero 9.355
Patrickios 4.342
IPC_peptide 9.326
IPC2_peptide 7.878
IPC2.peptide.svr19 7.734
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1201
98
569
240.2
27.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.83 ± 1.087
1.415 ± 0.716
7.41 ± 0.685
6.495 ± 0.772
4.913 ± 0.488
5.079 ± 0.631
1.582 ± 0.311
7.244 ± 0.468
9.992 ± 2.118
8.41 ± 0.414
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.165 ± 0.317
6.994 ± 0.682
3.997 ± 0.781
2.831 ± 0.758
3.164 ± 0.553
6.911 ± 0.887
5.495 ± 0.826
5.329 ± 0.783
1.166 ± 0.493
5.579 ± 0.863
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here