Gordonia phage Schiebs

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 25 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6TQH0|A0A5J6TQH0_9CAUD Portal protein OS=Gordonia phage Schiebs OX=2599850 GN=4 PE=4 SV=1
MM1 pKa = 7.66PGSTPNFGLTYY12 pKa = 10.37PLTTDD17 pKa = 5.19PIYY20 pKa = 10.77QGAAQLKK27 pKa = 8.47AAMEE31 pKa = 4.27RR32 pKa = 11.84ADD34 pKa = 3.75TVLKK38 pKa = 10.82SFQTYY43 pKa = 7.91IHH45 pKa = 7.42GIPAGPEE52 pKa = 4.34GPVGPAGPVGPVGPEE67 pKa = 4.19GPVGPAGPVGPIGAGLNLDD86 pKa = 4.0GQVPTYY92 pKa = 11.32ADD94 pKa = 4.1LPALGPADD102 pKa = 4.11VGAQYY107 pKa = 11.38AAGGLLYY114 pKa = 10.39VFEE117 pKa = 4.89GAWPPEE123 pKa = 4.0AEE125 pKa = 4.99GIPLVGPAGPVGPVGPEE142 pKa = 3.96GPAGPEE148 pKa = 4.38GPVGPAGPAGEE159 pKa = 4.3VTAAALDD166 pKa = 3.66ARR168 pKa = 11.84LGGLTFRR175 pKa = 11.84SSTTAPAAGTPASTITFVTTAA196 pKa = 3.78

Molecular weight: 18.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6TQJ7|A0A5J6TQJ7_9CAUD Minor tail protein OS=Gordonia phage Schiebs OX=2599850 GN=16 PE=4 SV=1
MM1 pKa = 7.62AEE3 pKa = 3.77VRR5 pKa = 11.84IEE7 pKa = 3.87GGARR11 pKa = 11.84LRR13 pKa = 11.84RR14 pKa = 11.84TLKK17 pKa = 10.78AAGVDD22 pKa = 3.63MKK24 pKa = 10.55EE25 pKa = 3.73LSAINRR31 pKa = 11.84KK32 pKa = 8.5AANVVATAARR42 pKa = 11.84PTAPVRR48 pKa = 11.84SGALARR54 pKa = 11.84SVRR57 pKa = 11.84AGATAKK63 pKa = 10.64AGVVRR68 pKa = 11.84AGNKK72 pKa = 8.46AVPYY76 pKa = 9.7AGPIHH81 pKa = 6.58WGWPARR87 pKa = 11.84GIEE90 pKa = 4.01PHH92 pKa = 6.47PWIADD97 pKa = 3.39AAQATEE103 pKa = 4.43SEE105 pKa = 4.29WVAQYY110 pKa = 10.56FEE112 pKa = 4.5HH113 pKa = 8.24VEE115 pKa = 4.08DD116 pKa = 4.15VLDD119 pKa = 4.02SVKK122 pKa = 10.64GRR124 pKa = 3.68

Molecular weight: 13.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

25

0

25

4694

38

641

187.8

19.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

16.042 ± 1.019

0.703 ± 0.236

6.114 ± 0.653

4.069 ± 0.384

2.322 ± 0.246

9.054 ± 0.673

1.64 ± 0.276

4.367 ± 0.524

1.555 ± 0.225

8.522 ± 0.656

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.896 ± 0.153

2.301 ± 0.297

8.053 ± 0.546

2.94 ± 0.236

6.37 ± 0.894

4.857 ± 0.462

8.074 ± 0.574

7.648 ± 0.456

1.875 ± 0.351

1.598 ± 0.317
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski