Amino acid dipepetide frequency for Geobacillus sp. (strain WCH70)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.714AlaAla: 6.714 ± 0.128	0.727AlaCys: 0.727 ± 0.03	3.314AlaAsp: 3.314 ± 0.059	5.118AlaGlu: 5.118 ± 0.084	3.404AlaPhe: 3.404 ± 0.071	5.112AlaGly: 5.112 ± 0.096	1.474AlaHis: 1.474 ± 0.042	6.209AlaIle: 6.209 ± 0.08	5.881AlaLys: 5.881 ± 0.103	8.027AlaLeu: 8.027 ± 0.115	2.241AlaMet: 2.241 ± 0.054	2.872AlaAsn: 2.872 ± 0.067	2.341AlaPro: 2.341 ± 0.055	2.512AlaGln: 2.512 ± 0.054	3.327AlaArg: 3.327 ± 0.069	3.836AlaSer: 3.836 ± 0.071	3.402AlaThr: 3.402 ± 0.059	5.822AlaVal: 5.822 ± 0.082	0.688AlaTrp: 0.688 ± 0.031	2.497AlaTyr: 2.497 ± 0.05	0.0AlaXaa: 0.0 ± 0.0
Cys	0.487CysAla: 0.487 ± 0.023	0.135CysCys: 0.135 ± 0.013	0.415CysAsp: 0.415 ± 0.024	0.558CysGlu: 0.558 ± 0.022	0.389CysPhe: 0.389 ± 0.019	0.797CysGly: 0.797 ± 0.039	0.262CysHis: 0.262 ± 0.019	0.634CysIle: 0.634 ± 0.026	0.404CysLys: 0.404 ± 0.022	0.769CysLeu: 0.769 ± 0.031	0.18CysMet: 0.18 ± 0.012	0.285CysAsn: 0.285 ± 0.017	0.473CysPro: 0.473 ± 0.023	0.278CysGln: 0.278 ± 0.018	0.459CysArg: 0.459 ± 0.023	0.489CysSer: 0.489 ± 0.027	0.463CysThr: 0.463 ± 0.022	0.502CysVal: 0.502 ± 0.025	0.087CysTrp: 0.087 ± 0.01	0.301CysTyr: 0.301 ± 0.018	0.0CysXaa: 0.0 ± 0.0
Asp	3.21AspAla: 3.21 ± 0.055	0.406AspCys: 0.406 ± 0.025	2.456AspAsp: 2.456 ± 0.06	4.459AspGlu: 4.459 ± 0.085	2.051AspPhe: 2.051 ± 0.052	3.322AspGly: 3.322 ± 0.066	1.149AspHis: 1.149 ± 0.033	4.112AspIle: 4.112 ± 0.069	2.475AspLys: 2.475 ± 0.05	4.355AspLeu: 4.355 ± 0.077	1.138AspMet: 1.138 ± 0.037	1.388AspAsn: 1.388 ± 0.044	2.07AspPro: 2.07 ± 0.047	1.648AspGln: 1.648 ± 0.047	2.448AspArg: 2.448 ± 0.056	1.922AspSer: 1.922 ± 0.051	1.991AspThr: 1.991 ± 0.041	4.079AspVal: 4.079 ± 0.083	0.597AspTrp: 0.597 ± 0.029	2.031AspTyr: 2.031 ± 0.051	0.0AspXaa: 0.0 ± 0.0
Glu	6.135GluAla: 6.135 ± 0.099	0.399GluCys: 0.399 ± 0.021	3.062GluAsp: 3.062 ± 0.068	7.435GluGlu: 7.435 ± 0.117	2.46GluPhe: 2.46 ± 0.061	4.027GluGly: 4.027 ± 0.068	1.755GluHis: 1.755 ± 0.044	5.418GluIle: 5.418 ± 0.098	7.26GluLys: 7.26 ± 0.108	7.123GluLeu: 7.123 ± 0.094	2.375GluMet: 2.375 ± 0.06	3.242GluAsn: 3.242 ± 0.063	1.972GluPro: 1.972 ± 0.048	4.258GluGln: 4.258 ± 0.093	4.692GluArg: 4.692 ± 0.091	2.776GluSer: 2.776 ± 0.057	4.164GluThr: 4.164 ± 0.071	4.994GluVal: 4.994 ± 0.103	0.959GluTrp: 0.959 ± 0.034	2.295GluTyr: 2.295 ± 0.058	0.0GluXaa: 0.0 ± 0.0
Phe	3.51PheAla: 3.51 ± 0.057	0.449PheCys: 0.449 ± 0.021	2.136PheAsp: 2.136 ± 0.05	2.537PheGlu: 2.537 ± 0.054	2.445PhePhe: 2.445 ± 0.073	3.203PheGly: 3.203 ± 0.073	1.198PheHis: 1.198 ± 0.037	3.507PheIle: 3.507 ± 0.074	1.784PheLys: 1.784 ± 0.044	4.635PheLeu: 4.635 ± 0.1	1.031PheMet: 1.031 ± 0.033	1.261PheAsn: 1.261 ± 0.043	1.827PhePro: 1.827 ± 0.049	1.689PheGln: 1.689 ± 0.05	1.976PheArg: 1.976 ± 0.048	2.97PheSer: 2.97 ± 0.058	2.493PheThr: 2.493 ± 0.053	3.3PheVal: 3.3 ± 0.06	0.497PheTrp: 0.497 ± 0.027	1.743PheTyr: 1.743 ± 0.05	0.0PheXaa: 0.0 ± 0.0
Gly	4.817GlyAla: 4.817 ± 0.094	0.649GlyCys: 0.649 ± 0.031	3.007GlyAsp: 3.007 ± 0.058	4.374GlyGlu: 4.374 ± 0.072	3.121GlyPhe: 3.121 ± 0.069	4.486GlyGly: 4.486 ± 0.089	1.373GlyHis: 1.373 ± 0.049	5.672GlyIle: 5.672 ± 0.089	5.342GlyLys: 5.342 ± 0.081	6.053GlyLeu: 6.053 ± 0.094	1.988GlyMet: 1.988 ± 0.057	2.451GlyAsn: 2.451 ± 0.056	1.738GlyPro: 1.738 ± 0.044	2.136GlyGln: 2.136 ± 0.057	3.038GlyArg: 3.038 ± 0.07	3.133GlySer: 3.133 ± 0.06	3.828GlyThr: 3.828 ± 0.075	5.134GlyVal: 5.134 ± 0.074	0.788GlyTrp: 0.788 ± 0.031	2.812GlyTyr: 2.812 ± 0.06	0.0GlyXaa: 0.0 ± 0.0
His	1.49HisAla: 1.49 ± 0.047	0.264HisCys: 0.264 ± 0.018	1.085HisAsp: 1.085 ± 0.03	1.637HisGlu: 1.637 ± 0.048	1.047HisPhe: 1.047 ± 0.037	1.597HisGly: 1.597 ± 0.053	0.881HisHis: 0.881 ± 0.041	1.922HisIle: 1.922 ± 0.055	0.984HisLys: 0.984 ± 0.037	2.38HisLeu: 2.38 ± 0.058	0.579HisMet: 0.579 ± 0.026	0.71HisAsn: 0.71 ± 0.028	1.399HisPro: 1.399 ± 0.046	0.894HisGln: 0.894 ± 0.033	1.272HisArg: 1.272 ± 0.041	1.198HisSer: 1.198 ± 0.04	1.006HisThr: 1.006 ± 0.035	1.797HisVal: 1.797 ± 0.048	0.259HisTrp: 0.259 ± 0.019	0.986HisTyr: 0.986 ± 0.037	0.0HisXaa: 0.0 ± 0.0
Ile	6.682IleAla: 6.682 ± 0.1	0.705IleCys: 0.705 ± 0.03	4.363IleAsp: 4.363 ± 0.087	5.959IleGlu: 5.959 ± 0.096	3.038IlePhe: 3.038 ± 0.056	5.921IleGly: 5.921 ± 0.096	1.831IleHis: 1.831 ± 0.043	5.633IleIle: 5.633 ± 0.096	4.045IleLys: 4.045 ± 0.081	6.108IleLeu: 6.108 ± 0.088	1.559IleMet: 1.559 ± 0.042	2.525IleAsn: 2.525 ± 0.062	3.606IlePro: 3.606 ± 0.066	2.841IleGln: 2.841 ± 0.052	4.002IleArg: 4.002 ± 0.069	4.401IleSer: 4.401 ± 0.067	3.969IleThr: 3.969 ± 0.072	6.042IleVal: 6.042 ± 0.086	0.694IleTrp: 0.694 ± 0.029	2.432IleTyr: 2.432 ± 0.052	0.0IleXaa: 0.0 ± 0.0
Lys	5.222LysAla: 5.222 ± 0.093	0.345LysCys: 0.345 ± 0.022	3.132LysAsp: 3.132 ± 0.066	7.264LysGlu: 7.264 ± 0.104	1.793LysPhe: 1.793 ± 0.046	4.426LysGly: 4.426 ± 0.066	1.488LysHis: 1.488 ± 0.038	4.334LysIle: 4.334 ± 0.076	6.016LysLys: 6.016 ± 0.1	5.859LysLeu: 5.859 ± 0.082	2.024LysMet: 2.024 ± 0.052	2.748LysAsn: 2.748 ± 0.068	2.31LysPro: 2.31 ± 0.053	4.051LysGln: 4.051 ± 0.078	4.327LysArg: 4.327 ± 0.076	2.812LysSer: 2.812 ± 0.054	3.87LysThr: 3.87 ± 0.067	4.406LysVal: 4.406 ± 0.076	0.889LysTrp: 0.889 ± 0.031	2.201LysTyr: 2.201 ± 0.054	0.0LysXaa: 0.0 ± 0.0
Leu	8.034LeuAla: 8.034 ± 0.1	0.89LeuCys: 0.89 ± 0.036	4.444LeuAsp: 4.444 ± 0.074	6.917LeuGlu: 6.917 ± 0.108	4.841LeuPhe: 4.841 ± 0.097	6.038LeuGly: 6.038 ± 0.082	2.354LeuHis: 2.354 ± 0.057	6.482LeuIle: 6.482 ± 0.111	6.218LeuLys: 6.218 ± 0.084	10.429LeuLeu: 10.429 ± 0.156	2.219LeuMet: 2.219 ± 0.047	3.251LeuAsn: 3.251 ± 0.066	4.209LeuPro: 4.209 ± 0.078	4.203LeuGln: 4.203 ± 0.075	4.725LeuArg: 4.725 ± 0.076	6.252LeuSer: 6.252 ± 0.104	5.02LeuThr: 5.02 ± 0.076	6.351LeuVal: 6.351 ± 0.089	0.868LeuTrp: 0.868 ± 0.037	3.343LeuTyr: 3.343 ± 0.072	0.0LeuXaa: 0.0 ± 0.0
Met	2.13MetAla: 2.13 ± 0.049	0.14MetCys: 0.14 ± 0.009	1.266MetAsp: 1.266 ± 0.035	2.165MetGlu: 2.165 ± 0.05	1.025MetPhe: 1.025 ± 0.036	1.528MetGly: 1.528 ± 0.037	0.473MetHis: 0.473 ± 0.024	2.117MetIle: 2.117 ± 0.054	2.553MetLys: 2.553 ± 0.052	2.379MetLeu: 2.379 ± 0.047	1.038MetMet: 1.038 ± 0.035	1.444MetAsn: 1.444 ± 0.039	1.019MetPro: 1.019 ± 0.039	0.859MetGln: 0.859 ± 0.031	1.252MetArg: 1.252 ± 0.034	1.374MetSer: 1.374 ± 0.044	1.605MetThr: 1.605 ± 0.042	1.751MetVal: 1.751 ± 0.035	0.19MetTrp: 0.19 ± 0.015	0.807MetTyr: 0.807 ± 0.034	0.0MetXaa: 0.0 ± 0.0
Asn	2.659AsnAla: 2.659 ± 0.055	0.242AsnCys: 0.242 ± 0.015	1.953AsnAsp: 1.953 ± 0.051	3.284AsnGlu: 3.284 ± 0.057	1.314AsnPhe: 1.314 ± 0.039	3.098AsnGly: 3.098 ± 0.066	0.869AsnHis: 0.869 ± 0.033	3.145AsnIle: 3.145 ± 0.061	2.361AsnLys: 2.361 ± 0.058	2.818AsnLeu: 2.818 ± 0.059	1.075AsnMet: 1.075 ± 0.039	1.527AsnAsn: 1.527 ± 0.056	1.972AsnPro: 1.972 ± 0.052	1.366AsnGln: 1.366 ± 0.043	1.989AsnArg: 1.989 ± 0.056	1.606AsnSer: 1.606 ± 0.049	1.655AsnThr: 1.655 ± 0.049	2.882AsnVal: 2.882 ± 0.064	0.389AsnTrp: 0.389 ± 0.023	1.278AsnTyr: 1.278 ± 0.046	0.0AsnXaa: 0.0 ± 0.0
Pro	2.332ProAla: 2.332 ± 0.056	0.274ProCys: 0.274 ± 0.021	1.965ProAsp: 1.965 ± 0.046	2.923ProGlu: 2.923 ± 0.061	2.202ProPhe: 2.202 ± 0.049	2.266ProGly: 2.266 ± 0.052	1.015ProHis: 1.015 ± 0.036	2.929ProIle: 2.929 ± 0.053	2.628ProLys: 2.628 ± 0.053	4.016ProLeu: 4.016 ± 0.062	0.89ProMet: 0.89 ± 0.034	1.811ProAsn: 1.811 ± 0.046	1.29ProPro: 1.29 ± 0.045	1.124ProGln: 1.124 ± 0.033	1.387ProArg: 1.387 ± 0.039	2.463ProSer: 2.463 ± 0.059	1.931ProThr: 1.931 ± 0.047	2.917ProVal: 2.917 ± 0.056	0.42ProTrp: 0.42 ± 0.024	1.62ProTyr: 1.62 ± 0.044	0.0ProXaa: 0.0 ± 0.0
Gln	3.126GlnAla: 3.126 ± 0.069	0.283GlnCys: 0.283 ± 0.017	1.314GlnAsp: 1.314 ± 0.039	2.957GlnGlu: 2.957 ± 0.067	1.724GlnPhe: 1.724 ± 0.044	2.224GlnGly: 2.224 ± 0.049	0.982GlnHis: 0.982 ± 0.032	2.706GlnIle: 2.706 ± 0.054	3.088GlnLys: 3.088 ± 0.071	4.623GlnLeu: 4.623 ± 0.083	1.24GlnMet: 1.24 ± 0.037	1.347GlnAsn: 1.347 ± 0.034	1.316GlnPro: 1.316 ± 0.043	2.349GlnGln: 2.349 ± 0.068	2.128GlnArg: 2.128 ± 0.058	1.926GlnSer: 1.926 ± 0.043	2.159GlnThr: 2.159 ± 0.046	2.19GlnVal: 2.19 ± 0.058	0.574GlnTrp: 0.574 ± 0.025	1.492GlnTyr: 1.492 ± 0.043	0.0GlnXaa: 0.0 ± 0.0
Arg	3.217ArgAla: 3.217 ± 0.073	0.424ArgCys: 0.424 ± 0.027	2.367ArgAsp: 2.367 ± 0.058	3.903ArgGlu: 3.903 ± 0.083	2.428ArgPhe: 2.428 ± 0.051	2.809ArgGly: 2.809 ± 0.057	1.231ArgHis: 1.231 ± 0.046	3.678ArgIle: 3.678 ± 0.073	3.841ArgLys: 3.841 ± 0.066	5.255ArgLeu: 5.255 ± 0.082	1.528ArgMet: 1.528 ± 0.039	1.961ArgAsn: 1.961 ± 0.05	1.725ArgPro: 1.725 ± 0.042	2.287ArgGln: 2.287 ± 0.056	2.784ArgArg: 2.784 ± 0.068	2.424ArgSer: 2.424 ± 0.056	2.422ArgThr: 2.422 ± 0.049	3.18ArgVal: 3.18 ± 0.065	0.63ArgTrp: 0.63 ± 0.027	2.115ArgTyr: 2.115 ± 0.047	0.0ArgXaa: 0.0 ± 0.0
Ser	3.448SerAla: 3.448 ± 0.065	0.412SerCys: 0.412 ± 0.022	2.429SerAsp: 2.429 ± 0.06	3.402SerGlu: 3.402 ± 0.072	2.96SerPhe: 2.96 ± 0.064	3.723SerGly: 3.723 ± 0.065	1.181SerHis: 1.181 ± 0.038	4.139SerIle: 4.139 ± 0.066	3.116SerLys: 3.116 ± 0.062	5.796SerLeu: 5.796 ± 0.092	1.435SerMet: 1.435 ± 0.039	1.801SerAsn: 1.801 ± 0.043	2.104SerPro: 2.104 ± 0.052	1.599SerGln: 1.599 ± 0.047	2.314SerArg: 2.314 ± 0.049	3.113SerSer: 3.113 ± 0.071	2.44SerThr: 2.44 ± 0.06	3.744SerVal: 3.744 ± 0.075	0.573SerTrp: 0.573 ± 0.028	1.996SerTyr: 1.996 ± 0.054	0.0SerXaa: 0.0 ± 0.0
Thr	3.856ThrAla: 3.856 ± 0.075	0.442ThrCys: 0.442 ± 0.023	2.369ThrAsp: 2.369 ± 0.05	3.29ThrGlu: 3.29 ± 0.061	2.533ThrPhe: 2.533 ± 0.049	3.791ThrGly: 3.791 ± 0.063	0.988ThrHis: 0.988 ± 0.035	4.491ThrIle: 4.491 ± 0.076	3.632ThrLys: 3.632 ± 0.066	5.032ThrLeu: 5.032 ± 0.075	1.408ThrMet: 1.408 ± 0.039	2.232ThrAsn: 2.232 ± 0.049	2.258ThrPro: 2.258 ± 0.053	1.157ThrGln: 1.157 ± 0.036	1.85ThrArg: 1.85 ± 0.049	2.633ThrSer: 2.633 ± 0.056	2.731ThrThr: 2.731 ± 0.064	4.344ThrVal: 4.344 ± 0.081	0.556ThrTrp: 0.556 ± 0.026	1.954ThrTyr: 1.954 ± 0.043	0.0ThrXaa: 0.0 ± 0.0
Val	5.419ValAla: 5.419 ± 0.089	0.698ValCys: 0.698 ± 0.029	3.689ValAsp: 3.689 ± 0.068	4.892ValGlu: 4.892 ± 0.083	3.087ValPhe: 3.087 ± 0.064	4.423ValGly: 4.423 ± 0.081	1.639ValHis: 1.639 ± 0.047	5.765ValIle: 5.765 ± 0.084	4.889ValLys: 4.889 ± 0.079	6.835ValLeu: 6.835 ± 0.085	1.9ValMet: 1.9 ± 0.045	2.798ValAsn: 2.798 ± 0.057	3.058ValPro: 3.058 ± 0.063	2.663ValGln: 2.663 ± 0.06	3.506ValArg: 3.506 ± 0.076	4.07ValSer: 4.07 ± 0.067	4.093ValThr: 4.093 ± 0.07	5.332ValVal: 5.332 ± 0.091	0.707ValTrp: 0.707 ± 0.027	2.544ValTyr: 2.544 ± 0.05	0.0ValXaa: 0.0 ± 0.0
Trp	0.667TrpAla: 0.667 ± 0.03	0.088TrpCys: 0.088 ± 0.01	0.492TrpAsp: 0.492 ± 0.024	0.79TrpGlu: 0.79 ± 0.028	0.536TrpPhe: 0.536 ± 0.027	0.582TrpGly: 0.582 ± 0.029	0.241TrpHis: 0.241 ± 0.018	0.873TrpIle: 0.873 ± 0.033	1.002TrpLys: 1.002 ± 0.036	1.263TrpLeu: 1.263 ± 0.046	0.387TrpMet: 0.387 ± 0.021	0.548TrpAsn: 0.548 ± 0.025	0.255TrpPro: 0.255 ± 0.02	0.446TrpGln: 0.446 ± 0.023	0.531TrpArg: 0.531 ± 0.027	0.518TrpSer: 0.518 ± 0.023	0.56TrpThr: 0.56 ± 0.028	0.645TrpVal: 0.645 ± 0.031	0.147TrpTrp: 0.147 ± 0.012	0.369TrpTyr: 0.369 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.362TyrAla: 2.362 ± 0.051	0.396TyrCys: 0.396 ± 0.017	2.048TyrAsp: 2.048 ± 0.048	2.94TyrGlu: 2.94 ± 0.06	1.795TyrPhe: 1.795 ± 0.053	2.565TyrGly: 2.565 ± 0.055	1.002TyrHis: 1.002 ± 0.039	2.589TyrIle: 2.589 ± 0.061	1.997TyrLys: 1.997 ± 0.055	3.333TyrLeu: 3.333 ± 0.067	0.879TyrMet: 0.879 ± 0.029	1.279TyrAsn: 1.279 ± 0.039	1.46TyrPro: 1.46 ± 0.045	1.389TyrGln: 1.389 ± 0.042	2.203TyrArg: 2.203 ± 0.049	1.811TyrSer: 1.811 ± 0.05	1.742TyrThr: 1.742 ± 0.038	2.596TyrVal: 2.596 ± 0.058	0.427TyrTrp: 0.427 ± 0.023	1.534TyrTyr: 1.534 ± 0.05	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3048 proteins (882799 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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