Pseudanabaena sp. PCC 7367

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Pseudanabaenaceae; Pseudanabaena; unclassified Pseudanabaena

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3824 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K9SIH3|K9SIH3_9CYAN Putative cyanate ABC transporter substrate binding protein OS=Pseudanabaena sp. PCC 7367 OX=82654 GN=Pse7367_1717 PE=4 SV=1
MM1 pKa = 7.37SGSHH5 pKa = 6.33NPLEE9 pKa = 4.3AHH11 pKa = 7.28PSPTQAHH18 pKa = 6.49AFSPIPANTSVTNGSTSQVPNLAASQGAVDD48 pKa = 4.02FAFVQRR54 pKa = 11.84QGDD57 pKa = 3.51AYY59 pKa = 10.62YY60 pKa = 10.24PNNTGFFVGGDD71 pKa = 2.89IYY73 pKa = 11.11TPLVNDD79 pKa = 3.85VSTGGALGFLDD90 pKa = 5.87FYY92 pKa = 11.15VLPRR96 pKa = 11.84DD97 pKa = 3.53AVPQHH102 pKa = 4.94THH104 pKa = 4.2AHH106 pKa = 4.94EE107 pKa = 5.01AEE109 pKa = 3.91AKK111 pKa = 9.14YY112 pKa = 10.77VLEE115 pKa = 4.49GEE117 pKa = 4.74VNFDD121 pKa = 4.53LGPAQIRR128 pKa = 11.84VPTGSFIYY136 pKa = 10.48YY137 pKa = 9.8PIGRR141 pKa = 11.84QMGFTATDD149 pKa = 3.18QSARR153 pKa = 11.84ISVITTPGAPYY164 pKa = 10.76YY165 pKa = 9.81EE166 pKa = 4.38FAGVAVVDD174 pKa = 4.03EE175 pKa = 4.69QGNNTPPPQADD186 pKa = 2.84IGAIAAQLDD195 pKa = 4.08FGVVGQIIDD204 pKa = 3.88TYY206 pKa = 11.05GGGAFIPGVDD216 pKa = 3.39SAFQAGLQSPLLVVPDD232 pKa = 4.95LDD234 pKa = 6.2LIDD237 pKa = 4.43PAQLEE242 pKa = 4.31AVRR245 pKa = 11.84NVPGLNLQIFEE256 pKa = 3.96ISDD259 pKa = 3.24RR260 pKa = 11.84RR261 pKa = 11.84KK262 pKa = 9.68FDD264 pKa = 3.88GLFGTQNTSLIDD276 pKa = 3.8FEE278 pKa = 5.04EE279 pKa = 5.29SDD281 pKa = 4.01GNFAYY286 pKa = 10.63SQFNLARR293 pKa = 11.84DD294 pKa = 4.07DD295 pKa = 3.7RR296 pKa = 11.84HH297 pKa = 6.65SSNFLQAVINSDD309 pKa = 4.03QIVTTDD315 pKa = 3.28GVGVDD320 pKa = 3.86SGAFAYY326 pKa = 10.21ARR328 pKa = 11.84LHH330 pKa = 6.36AEE332 pKa = 3.85PNGTITYY339 pKa = 8.05EE340 pKa = 3.69ITINGLDD347 pKa = 3.2AGAYY351 pKa = 10.13LGDD354 pKa = 4.0GTPFTPDD361 pKa = 3.42PGDD364 pKa = 4.1DD365 pKa = 3.11LTAIHH370 pKa = 6.97LHH372 pKa = 4.67TAFRR376 pKa = 11.84GEE378 pKa = 4.27NGTHH382 pKa = 7.06AFNILGPGDD391 pKa = 4.31DD392 pKa = 4.89ADD394 pKa = 4.11LTVHH398 pKa = 6.43GNADD402 pKa = 3.47GSVTFSGVWDD412 pKa = 4.82DD413 pKa = 5.6SDD415 pKa = 3.85TTTDD419 pKa = 3.55TLPPPMSTKK428 pKa = 10.01PVSNFLPTLSNAAVGEE444 pKa = 4.12DD445 pKa = 2.78VGLYY449 pKa = 10.65VNIHH453 pKa = 5.6SNRR456 pKa = 11.84NSSGEE461 pKa = 3.48IRR463 pKa = 11.84GQVVGTTDD471 pKa = 3.98AFPASQVADD480 pKa = 3.35NHH482 pKa = 5.71LTFLVQEE489 pKa = 4.64GEE491 pKa = 4.28VAFQVNGNPFVAGVDD506 pKa = 3.5DD507 pKa = 4.23AVYY510 pKa = 10.16IAAGQNYY517 pKa = 10.22SIANLGNQNAVGLAAAVVDD536 pKa = 4.24SYY538 pKa = 11.68VPKK541 pKa = 10.33IGEE544 pKa = 4.29YY545 pKa = 10.28YY546 pKa = 10.58DD547 pKa = 4.07GNIDD551 pKa = 3.54FTASIRR557 pKa = 11.84TGDD560 pKa = 3.66EE561 pKa = 4.45FIQGNVVTTVPQAIGNGWIYY581 pKa = 9.81TWGAFGTDD589 pKa = 3.2DD590 pKa = 4.61FGNEE594 pKa = 3.88VPTSIGVTFTEE605 pKa = 4.96EE606 pKa = 3.68ALQDD610 pKa = 3.73AFVVDD615 pKa = 4.41DD616 pKa = 4.95PNNEE620 pKa = 4.02FPSSVPHH627 pKa = 6.48LAFPEE632 pKa = 3.89IFEE635 pKa = 4.12AARR638 pKa = 11.84VYY640 pKa = 11.05NILFPEE646 pKa = 4.87KK647 pKa = 9.92VQQSTPFNHH656 pKa = 6.22MGFYY660 pKa = 10.85ANSEE664 pKa = 3.81GHH666 pKa = 6.99APLDD670 pKa = 4.1IYY672 pKa = 10.93DD673 pKa = 4.46LPHH676 pKa = 6.7FDD678 pKa = 3.22VHH680 pKa = 6.72FFLDD684 pKa = 4.28TIEE687 pKa = 4.16EE688 pKa = 4.15RR689 pKa = 11.84DD690 pKa = 4.41LITGLPEE697 pKa = 5.02DD698 pKa = 3.99NANLFNLPEE707 pKa = 4.92PGFLPANYY715 pKa = 9.09IAPTIPGTDD724 pKa = 3.04IPATGDD730 pKa = 3.4ALQGIHH736 pKa = 5.39WVAGEE741 pKa = 4.01TPEE744 pKa = 4.49FNGEE748 pKa = 4.03VFDD751 pKa = 3.6QTFIFGSYY759 pKa = 10.27AGDD762 pKa = 3.53VNFWEE767 pKa = 4.27PMITRR772 pKa = 11.84DD773 pKa = 3.77FLEE776 pKa = 4.5SLSSARR782 pKa = 11.84QVITEE787 pKa = 3.9TTYY790 pKa = 11.27NIDD793 pKa = 2.98QPTRR797 pKa = 11.84FKK799 pKa = 11.0EE800 pKa = 3.81AGFYY804 pKa = 9.52PLEE807 pKa = 4.08YY808 pKa = 10.23GISYY812 pKa = 9.27NANHH816 pKa = 7.04GEE818 pKa = 4.21YY819 pKa = 10.0TVTLDD824 pKa = 3.28NFVIRR829 pKa = 11.84SADD832 pKa = 3.34GDD834 pKa = 3.78LSGFPGFDD842 pKa = 3.3GPPPFF847 pKa = 5.45

Molecular weight: 90.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K9SKN6|K9SKN6_9CYAN Uncharacterized protein OS=Pseudanabaena sp. PCC 7367 OX=82654 GN=Pse7367_2081 PE=4 SV=1
MM1 pKa = 7.54APKK4 pKa = 10.19DD5 pKa = 3.57YY6 pKa = 10.34RR7 pKa = 11.84RR8 pKa = 11.84CISCRR13 pKa = 11.84RR14 pKa = 11.84LDD16 pKa = 3.77HH17 pKa = 7.25RR18 pKa = 11.84DD19 pKa = 3.21HH20 pKa = 6.82FWRR23 pKa = 11.84VVRR26 pKa = 11.84SRR28 pKa = 11.84DD29 pKa = 3.24SSGQISVQLDD39 pKa = 3.08QGMGRR44 pKa = 11.84SAYY47 pKa = 8.73ICRR50 pKa = 11.84NADD53 pKa = 3.75CLKK56 pKa = 10.39QAQKK60 pKa = 10.82KK61 pKa = 9.0NRR63 pKa = 11.84LARR66 pKa = 11.84VLRR69 pKa = 11.84APVEE73 pKa = 3.74PTIYY77 pKa = 10.39EE78 pKa = 3.99RR79 pKa = 11.84LQARR83 pKa = 11.84LVAEE87 pKa = 4.01QAKK90 pKa = 10.38

Molecular weight: 10.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3824

0

3824

1251467

29

4259

327.3

36.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.329 ± 0.036

0.919 ± 0.016

5.767 ± 0.043

6.007 ± 0.038

3.76 ± 0.026

6.697 ± 0.044

1.836 ± 0.021

6.758 ± 0.026

4.337 ± 0.038

10.514 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.032 ± 0.02

4.424 ± 0.035

4.771 ± 0.029

5.312 ± 0.038

5.171 ± 0.032

6.398 ± 0.032

5.372 ± 0.04

6.403 ± 0.032

1.305 ± 0.017

2.887 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski