Amino acid dipepetide frequency for Firmicutes bacterium CAG:646

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.477AlaAla: 6.477 ± 0.127	1.028AlaCys: 1.028 ± 0.041	3.938AlaAsp: 3.938 ± 0.075	5.206AlaGlu: 5.206 ± 0.09	2.803AlaPhe: 2.803 ± 0.061	5.988AlaGly: 5.988 ± 0.117	1.048AlaHis: 1.048 ± 0.034	4.363AlaIle: 4.363 ± 0.075	4.399AlaLys: 4.399 ± 0.077	6.609AlaLeu: 6.609 ± 0.101	2.289AlaMet: 2.289 ± 0.052	2.31AlaAsn: 2.31 ± 0.07	1.987AlaPro: 1.987 ± 0.059	2.383AlaGln: 2.383 ± 0.058	2.93AlaArg: 2.93 ± 0.063	3.567AlaSer: 3.567 ± 0.07	2.858AlaThr: 2.858 ± 0.07	6.099AlaVal: 6.099 ± 0.099	0.701AlaTrp: 0.701 ± 0.032	2.67AlaTyr: 2.67 ± 0.067	0.002AlaXaa: 0.002 ± 0.002
Cys	0.939CysAla: 0.939 ± 0.038	0.329CysCys: 0.329 ± 0.022	0.733CysAsp: 0.733 ± 0.03	0.916CysGlu: 0.916 ± 0.035	0.573CysPhe: 0.573 ± 0.027	1.417CysGly: 1.417 ± 0.05	0.314CysHis: 0.314 ± 0.019	1.093CysIle: 1.093 ± 0.047	0.851CysLys: 0.851 ± 0.033	1.172CysLeu: 1.172 ± 0.042	0.501CysMet: 0.501 ± 0.025	0.541CysAsn: 0.541 ± 0.027	0.64CysPro: 0.64 ± 0.028	0.523CysGln: 0.523 ± 0.028	0.72CysArg: 0.72 ± 0.033	0.986CysSer: 0.986 ± 0.033	0.752CysThr: 0.752 ± 0.031	0.998CysVal: 0.998 ± 0.033	0.156CysTrp: 0.156 ± 0.015	0.579CysTyr: 0.579 ± 0.03	0.0CysXaa: 0.0 ± 0.0
Asp	3.617AspAla: 3.617 ± 0.065	0.797AspCys: 0.797 ± 0.029	2.274AspAsp: 2.274 ± 0.064	4.132AspGlu: 4.132 ± 0.07	2.614AspPhe: 2.614 ± 0.064	4.171AspGly: 4.171 ± 0.08	0.894AspHis: 0.894 ± 0.036	3.898AspIle: 3.898 ± 0.073	3.063AspLys: 3.063 ± 0.062	4.641AspLeu: 4.641 ± 0.08	1.685AspMet: 1.685 ± 0.041	1.783AspAsn: 1.783 ± 0.048	1.73AspPro: 1.73 ± 0.055	1.789AspGln: 1.789 ± 0.041	2.341AspArg: 2.341 ± 0.058	2.755AspSer: 2.755 ± 0.063	3.052AspThr: 3.052 ± 0.059	3.597AspVal: 3.597 ± 0.07	0.607AspTrp: 0.607 ± 0.031	2.504AspTyr: 2.504 ± 0.058	0.0AspXaa: 0.0 ± 0.0
Glu	5.427GluAla: 5.427 ± 0.1	0.853GluCys: 0.853 ± 0.032	4.41GluAsp: 4.41 ± 0.087	9.321GluGlu: 9.321 ± 0.144	2.718GluPhe: 2.718 ± 0.057	5.08GluGly: 5.08 ± 0.077	1.501GluHis: 1.501 ± 0.043	5.999GluIle: 5.999 ± 0.091	8.13GluLys: 8.13 ± 0.124	7.209GluLeu: 7.209 ± 0.107	2.661GluMet: 2.661 ± 0.059	4.522GluAsn: 4.522 ± 0.085	2.077GluPro: 2.077 ± 0.057	3.62GluGln: 3.62 ± 0.079	3.797GluArg: 3.797 ± 0.078	3.464GluSer: 3.464 ± 0.064	4.077GluThr: 4.077 ± 0.078	4.695GluVal: 4.695 ± 0.094	0.78GluTrp: 0.78 ± 0.032	3.446GluTyr: 3.446 ± 0.077	0.0GluXaa: 0.0 ± 0.0
Phe	2.78PheAla: 2.78 ± 0.061	0.797PheCys: 0.797 ± 0.036	2.365PheAsp: 2.365 ± 0.061	2.623PheGlu: 2.623 ± 0.055	2.023PhePhe: 2.023 ± 0.064	3.0PheGly: 3.0 ± 0.069	0.892PheHis: 0.892 ± 0.035	2.481PheIle: 2.481 ± 0.062	1.768PheLys: 1.768 ± 0.052	4.352PheLeu: 4.352 ± 0.096	1.184PheMet: 1.184 ± 0.043	1.263PheAsn: 1.263 ± 0.037	1.507PhePro: 1.507 ± 0.046	1.829PheGln: 1.829 ± 0.052	1.892PheArg: 1.892 ± 0.049	3.081PheSer: 3.081 ± 0.072	2.305PheThr: 2.305 ± 0.047	2.831PheVal: 2.831 ± 0.058	0.526PheTrp: 0.526 ± 0.025	1.817PheTyr: 1.817 ± 0.049	0.0PheXaa: 0.0 ± 0.0
Gly	4.994GlyAla: 4.994 ± 0.101	1.278GlyCys: 1.278 ± 0.049	3.448GlyAsp: 3.448 ± 0.081	5.657GlyGlu: 5.657 ± 0.098	3.216GlyPhe: 3.216 ± 0.077	4.934GlyGly: 4.934 ± 0.118	1.194GlyHis: 1.194 ± 0.039	6.433GlyIle: 6.433 ± 0.116	6.066GlyLys: 6.066 ± 0.087	5.679GlyLeu: 5.679 ± 0.099	2.633GlyMet: 2.633 ± 0.06	3.19GlyAsn: 3.19 ± 0.077	1.243GlyPro: 1.243 ± 0.045	2.294GlyGln: 2.294 ± 0.051	2.991GlyArg: 2.991 ± 0.059	3.877GlySer: 3.877 ± 0.076	4.066GlyThr: 4.066 ± 0.077	5.078GlyVal: 5.078 ± 0.086	0.908GlyTrp: 0.908 ± 0.049	3.264GlyTyr: 3.264 ± 0.066	0.002GlyXaa: 0.002 ± 0.002
His	0.986HisAla: 0.986 ± 0.035	0.321HisCys: 0.321 ± 0.023	0.853HisAsp: 0.853 ± 0.037	1.034HisGlu: 1.034 ± 0.038	0.89HisPhe: 0.89 ± 0.035	1.4HisGly: 1.4 ± 0.038	0.526HisHis: 0.526 ± 0.031	1.315HisIle: 1.315 ± 0.039	0.954HisLys: 0.954 ± 0.037	1.708HisLeu: 1.708 ± 0.049	0.592HisMet: 0.592 ± 0.024	0.67HisAsn: 0.67 ± 0.028	1.013HisPro: 1.013 ± 0.036	0.745HisGln: 0.745 ± 0.031	0.865HisArg: 0.865 ± 0.031	0.97HisSer: 0.97 ± 0.04	1.047HisThr: 1.047 ± 0.041	1.116HisVal: 1.116 ± 0.04	0.222HisTrp: 0.222 ± 0.016	0.785HisTyr: 0.785 ± 0.034	0.0HisXaa: 0.0 ± 0.0
Ile	4.909IleAla: 4.909 ± 0.084	1.348IleCys: 1.348 ± 0.05	3.515IleAsp: 3.515 ± 0.065	4.28IleGlu: 4.28 ± 0.082	3.355IlePhe: 3.355 ± 0.088	4.749IleGly: 4.749 ± 0.098	1.446IleHis: 1.446 ± 0.045	4.24IleIle: 4.24 ± 0.089	3.48IleLys: 3.48 ± 0.065	7.71IleLeu: 7.71 ± 0.123	1.786IleMet: 1.786 ± 0.048	2.492IleAsn: 2.492 ± 0.06	3.323IlePro: 3.323 ± 0.059	2.873IleGln: 2.873 ± 0.061	4.019IleArg: 4.019 ± 0.084	4.866IleSer: 4.866 ± 0.097	4.099IleThr: 4.099 ± 0.075	4.405IleVal: 4.405 ± 0.081	0.758IleTrp: 0.758 ± 0.033	2.747IleTyr: 2.747 ± 0.059	0.0IleXaa: 0.0 ± 0.0
Lys	4.746LysAla: 4.746 ± 0.08	0.671LysCys: 0.671 ± 0.025	3.746LysAsp: 3.746 ± 0.066	7.558LysGlu: 7.558 ± 0.101	1.921LysPhe: 1.921 ± 0.055	4.273LysGly: 4.273 ± 0.083	0.96LysHis: 0.96 ± 0.037	5.291LysIle: 5.291 ± 0.093	6.713LysLys: 6.713 ± 0.113	5.387LysLeu: 5.387 ± 0.093	2.372LysMet: 2.372 ± 0.048	3.647LysAsn: 3.647 ± 0.069	1.913LysPro: 1.913 ± 0.057	2.416LysGln: 2.416 ± 0.053	3.155LysArg: 3.155 ± 0.061	3.152LysSer: 3.152 ± 0.06	3.656LysThr: 3.656 ± 0.071	4.295LysVal: 4.295 ± 0.077	0.621LysTrp: 0.621 ± 0.026	2.806LysTyr: 2.806 ± 0.065	0.001LysXaa: 0.001 ± 0.001
Leu	6.663LeuAla: 6.663 ± 0.103	1.485LeuCys: 1.485 ± 0.047	4.964LeuAsp: 4.964 ± 0.083	7.512LeuGlu: 7.512 ± 0.122	3.778LeuPhe: 3.778 ± 0.086	6.332LeuGly: 6.332 ± 0.113	1.731LeuHis: 1.731 ± 0.043	5.715LeuIle: 5.715 ± 0.101	6.298LeuLys: 6.298 ± 0.085	9.282LeuLeu: 9.282 ± 0.166	2.635LeuMet: 2.635 ± 0.061	3.545LeuAsn: 3.545 ± 0.074	3.641LeuPro: 3.641 ± 0.068	3.293LeuGln: 3.293 ± 0.056	3.859LeuArg: 3.859 ± 0.075	5.786LeuSer: 5.786 ± 0.097	5.018LeuThr: 5.018 ± 0.075	5.841LeuVal: 5.841 ± 0.092	0.851LeuTrp: 0.851 ± 0.035	3.484LeuTyr: 3.484 ± 0.078	0.0LeuXaa: 0.0 ± 0.0
Met	2.38MetAla: 2.38 ± 0.062	0.315MetCys: 0.315 ± 0.023	1.818MetAsp: 1.818 ± 0.041	3.323MetGlu: 3.323 ± 0.062	0.985MetPhe: 0.985 ± 0.036	2.346MetGly: 2.346 ± 0.058	0.461MetHis: 0.461 ± 0.026	2.314MetIle: 2.314 ± 0.063	2.785MetLys: 2.785 ± 0.055	2.531MetLeu: 2.531 ± 0.054	1.016MetMet: 1.016 ± 0.033	1.49MetAsn: 1.49 ± 0.041	1.083MetPro: 1.083 ± 0.035	1.126MetGln: 1.126 ± 0.041	1.338MetArg: 1.338 ± 0.043	1.578MetSer: 1.578 ± 0.043	1.644MetThr: 1.644 ± 0.046	2.021MetVal: 2.021 ± 0.052	0.23MetTrp: 0.23 ± 0.017	0.92MetTyr: 0.92 ± 0.033	0.001MetXaa: 0.001 ± 0.001
Asn	2.671AsnAla: 2.671 ± 0.069	0.592AsnCys: 0.592 ± 0.027	1.784AsnAsp: 1.784 ± 0.051	2.529AsnGlu: 2.529 ± 0.057	1.624AsnPhe: 1.624 ± 0.05	3.377AsnGly: 3.377 ± 0.088	0.834AsnHis: 0.834 ± 0.034	3.03AsnIle: 3.03 ± 0.066	2.219AsnLys: 2.219 ± 0.05	3.825AsnLeu: 3.825 ± 0.067	1.339AsnMet: 1.339 ± 0.04	1.606AsnAsn: 1.606 ± 0.051	2.001AsnPro: 2.001 ± 0.046	1.557AsnGln: 1.557 ± 0.043	2.065AsnArg: 2.065 ± 0.053	2.179AsnSer: 2.179 ± 0.05	2.263AsnThr: 2.263 ± 0.059	2.586AsnVal: 2.586 ± 0.049	0.473AsnTrp: 0.473 ± 0.026	1.677AsnTyr: 1.677 ± 0.047	0.0AsnXaa: 0.0 ± 0.0
Pro	2.39ProAla: 2.39 ± 0.056	0.464ProCys: 0.464 ± 0.026	2.089ProAsp: 2.089 ± 0.054	3.739ProGlu: 3.739 ± 0.076	1.609ProPhe: 1.609 ± 0.048	2.465ProGly: 2.465 ± 0.063	0.624ProHis: 0.624 ± 0.03	1.967ProIle: 1.967 ± 0.046	1.784ProLys: 1.784 ± 0.046	2.895ProLeu: 2.895 ± 0.063	0.948ProMet: 0.948 ± 0.032	1.144ProAsn: 1.144 ± 0.038	0.706ProPro: 0.706 ± 0.032	1.212ProGln: 1.212 ± 0.042	1.156ProArg: 1.156 ± 0.033	1.796ProSer: 1.796 ± 0.047	1.563ProThr: 1.563 ± 0.054	3.136ProVal: 3.136 ± 0.068	0.399ProTrp: 0.399 ± 0.024	1.45ProTyr: 1.45 ± 0.047	0.0ProXaa: 0.0 ± 0.0
Gln	2.567GlnAla: 2.567 ± 0.058	0.375GlnCys: 0.375 ± 0.021	1.67GlnAsp: 1.67 ± 0.046	3.908GlnGlu: 3.908 ± 0.09	1.242GlnPhe: 1.242 ± 0.043	2.547GlnGly: 2.547 ± 0.052	0.514GlnHis: 0.514 ± 0.03	2.994GlnIle: 2.994 ± 0.068	3.169GlnLys: 3.169 ± 0.064	3.099GlnLeu: 3.099 ± 0.074	1.465GlnMet: 1.465 ± 0.049	1.611GlnAsn: 1.611 ± 0.041	1.043GlnPro: 1.043 ± 0.04	1.45GlnGln: 1.45 ± 0.051	1.624GlnArg: 1.624 ± 0.046	1.633GlnSer: 1.633 ± 0.04	1.862GlnThr: 1.862 ± 0.058	2.735GlnVal: 2.735 ± 0.052	0.437GlnTrp: 0.437 ± 0.024	1.424GlnTyr: 1.424 ± 0.045	0.0GlnXaa: 0.0 ± 0.0
Arg	2.713ArgAla: 2.713 ± 0.064	0.61ArgCys: 0.61 ± 0.026	2.122ArgAsp: 2.122 ± 0.057	4.66ArgGlu: 4.66 ± 0.092	1.945ArgPhe: 1.945 ± 0.054	2.639ArgGly: 2.639 ± 0.063	0.762ArgHis: 0.762 ± 0.033	3.635ArgIle: 3.635 ± 0.069	4.074ArgLys: 4.074 ± 0.078	3.903ArgLeu: 3.903 ± 0.084	1.712ArgMet: 1.712 ± 0.045	1.882ArgAsn: 1.882 ± 0.06	1.259ArgPro: 1.259 ± 0.043	1.881ArgGln: 1.881 ± 0.053	2.385ArgArg: 2.385 ± 0.072	2.095ArgSer: 2.095 ± 0.046	2.157ArgThr: 2.157 ± 0.058	2.741ArgVal: 2.741 ± 0.062	0.45ArgTrp: 0.45 ± 0.021	1.949ArgTyr: 1.949 ± 0.058	0.0ArgXaa: 0.0 ± 0.0
Ser	3.754SerAla: 3.754 ± 0.065	0.841SerCys: 0.841 ± 0.033	2.798SerAsp: 2.798 ± 0.076	3.853SerGlu: 3.853 ± 0.07	2.58SerPhe: 2.58 ± 0.063	4.697SerGly: 4.697 ± 0.076	1.091SerHis: 1.091 ± 0.036	3.627SerIle: 3.627 ± 0.074	2.967SerLys: 2.967 ± 0.069	5.033SerLeu: 5.033 ± 0.092	1.812SerMet: 1.812 ± 0.046	1.852SerAsn: 1.852 ± 0.05	1.784SerPro: 1.784 ± 0.045	2.159SerGln: 2.159 ± 0.053	2.84SerArg: 2.84 ± 0.056	3.424SerSer: 3.424 ± 0.07	2.549SerThr: 2.549 ± 0.069	4.274SerVal: 4.274 ± 0.09	0.697SerTrp: 0.697 ± 0.029	2.42SerTyr: 2.42 ± 0.056	0.0SerXaa: 0.0 ± 0.0
Thr	4.033ThrAla: 4.033 ± 0.068	0.665ThrCys: 0.665 ± 0.033	2.861ThrAsp: 2.861 ± 0.071	4.133ThrGlu: 4.133 ± 0.076	2.106ThrPhe: 2.106 ± 0.043	4.559ThrGly: 4.559 ± 0.084	0.907ThrHis: 0.907 ± 0.03	3.606ThrIle: 3.606 ± 0.071	2.85ThrLys: 2.85 ± 0.054	4.799ThrLeu: 4.799 ± 0.068	1.448ThrMet: 1.448 ± 0.052	1.816ThrAsn: 1.816 ± 0.055	2.322ThrPro: 2.322 ± 0.066	1.647ThrGln: 1.647 ± 0.051	2.019ThrArg: 2.019 ± 0.048	2.812ThrSer: 2.812 ± 0.059	2.669ThrThr: 2.669 ± 0.062	4.381ThrVal: 4.381 ± 0.075	0.561ThrTrp: 0.561 ± 0.029	2.171ThrTyr: 2.171 ± 0.052	0.0ThrXaa: 0.0 ± 0.0
Val	4.43ValAla: 4.43 ± 0.075	1.21ValCys: 1.21 ± 0.039	3.651ValAsp: 3.651 ± 0.061	5.265ValGlu: 5.265 ± 0.082	3.039ValPhe: 3.039 ± 0.058	4.593ValGly: 4.593 ± 0.084	1.198ValHis: 1.198 ± 0.048	5.001ValIle: 5.001 ± 0.09	4.614ValLys: 4.614 ± 0.073	6.814ValLeu: 6.814 ± 0.112	2.003ValMet: 2.003 ± 0.048	2.737ValAsn: 2.737 ± 0.059	2.474ValPro: 2.474 ± 0.057	2.269ValGln: 2.269 ± 0.057	2.945ValArg: 2.945 ± 0.057	4.304ValSer: 4.304 ± 0.079	4.111ValThr: 4.111 ± 0.077	4.867ValVal: 4.867 ± 0.088	0.744ValTrp: 0.744 ± 0.035	2.65ValTyr: 2.65 ± 0.055	0.001ValXaa: 0.001 ± 0.001
Trp	0.599TrpAla: 0.599 ± 0.03	0.157TrpCys: 0.157 ± 0.015	0.579TrpAsp: 0.579 ± 0.026	0.924TrpGlu: 0.924 ± 0.038	0.438TrpPhe: 0.438 ± 0.025	0.718TrpGly: 0.718 ± 0.035	0.17TrpHis: 0.17 ± 0.015	0.812TrpIle: 0.812 ± 0.038	0.98TrpLys: 0.98 ± 0.036	1.001TrpLeu: 1.001 ± 0.035	0.468TrpMet: 0.468 ± 0.027	0.583TrpAsn: 0.583 ± 0.027	0.218TrpPro: 0.218 ± 0.016	0.416TrpGln: 0.416 ± 0.029	0.416TrpArg: 0.416 ± 0.023	0.513TrpSer: 0.513 ± 0.027	0.44TrpThr: 0.44 ± 0.025	0.604TrpVal: 0.604 ± 0.032	0.149TrpTrp: 0.149 ± 0.016	0.521TrpTyr: 0.521 ± 0.044	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.578TyrAla: 2.578 ± 0.053	0.598TyrCys: 0.598 ± 0.028	2.326TyrAsp: 2.326 ± 0.055	3.218TyrGlu: 3.218 ± 0.068	1.853TyrPhe: 1.853 ± 0.048	3.16TyrGly: 3.16 ± 0.062	0.945TyrHis: 0.945 ± 0.034	2.584TyrIle: 2.584 ± 0.057	2.136TyrLys: 2.136 ± 0.056	4.062TyrLeu: 4.062 ± 0.07	1.154TyrMet: 1.154 ± 0.04	1.548TyrAsn: 1.548 ± 0.046	1.572TyrPro: 1.572 ± 0.047	1.887TyrGln: 1.887 ± 0.053	2.217TyrArg: 2.217 ± 0.06	2.216TyrSer: 2.216 ± 0.047	2.174TyrThr: 2.174 ± 0.051	2.62TyrVal: 2.62 ± 0.054	0.436TyrTrp: 0.436 ± 0.021	1.931TyrTyr: 1.931 ± 0.061	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.001XaaMet: 0.001 ± 0.001	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.001	0.012XaaXaa: 0.012 ± 0.005

Statistics based on 2671 proteins (834769 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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