Parvimonas micra
Average proteome isoelectric point is 6.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1418 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B4S2J3|A0A0B4S2J3_9FIRM Methyltransferase OS=Parvimonas micra OX=33033 GN=NW74_04620 PE=4 SV=1
MM1 pKa = 7.73 EE2 pKa = 4.99 EE3 pKa = 4.47 NKK5 pKa = 10.74 GCGCGCGCEE14 pKa = 4.01 EE15 pKa = 4.2 EE16 pKa = 4.76 MEE18 pKa = 4.66 AEE20 pKa = 4.08 LLVFEE25 pKa = 5.09 NEE27 pKa = 4.23 DD28 pKa = 3.44 GKK30 pKa = 11.48 EE31 pKa = 3.83 EE32 pKa = 4.05 TFEE35 pKa = 4.23 VIGVFEE41 pKa = 4.4 VEE43 pKa = 4.1 EE44 pKa = 4.24 KK45 pKa = 10.76 EE46 pKa = 4.36 YY47 pKa = 10.61 IALQSQEE54 pKa = 4.48 DD55 pKa = 4.41 GSILLCKK62 pKa = 10.45 YY63 pKa = 10.01 IEE65 pKa = 4.47 KK66 pKa = 10.34 EE67 pKa = 3.78 DD68 pKa = 4.01 GEE70 pKa = 4.61 FEE72 pKa = 4.24 VEE74 pKa = 4.19 EE75 pKa = 5.09 IEE77 pKa = 5.48 DD78 pKa = 3.76 DD79 pKa = 3.96 EE80 pKa = 4.73 EE81 pKa = 4.38 FEE83 pKa = 4.37 KK84 pKa = 11.46 VSDD87 pKa = 3.77 AFDD90 pKa = 3.95 ALVEE94 pKa = 4.31 EE95 pKa = 5.28 DD96 pKa = 5.4 DD97 pKa = 4.05 EE98 pKa = 4.61
Molecular weight: 11.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.886
IPC2_protein 3.592
IPC_protein 3.516
Toseland 3.35
ProMoST 3.63
Dawson 3.478
Bjellqvist 3.668
Wikipedia 3.338
Rodwell 3.363
Grimsley 3.274
Solomon 3.452
Lehninger 3.401
Nozaki 3.592
DTASelect 3.681
Thurlkill 3.389
EMBOSS 3.363
Sillero 3.63
Patrickios 1.723
IPC_peptide 3.452
IPC2_peptide 3.617
IPC2.peptide.svr19 3.641
Protein with the highest isoelectric point:
>tr|A0A0B4RZK2|A0A0B4RZK2_9FIRM Membrane protein insertase YidC OS=Parvimonas micra OX=33033 GN=yidC PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.4 RR3 pKa = 11.84 TYY5 pKa = 10.21 QPNRR9 pKa = 11.84 RR10 pKa = 11.84 KK11 pKa = 9.79 RR12 pKa = 11.84 SKK14 pKa = 9.53 IHH16 pKa = 5.79 GFRR19 pKa = 11.84 KK20 pKa = 10.01 RR21 pKa = 11.84 MSTKK25 pKa = 10.24 AGRR28 pKa = 11.84 AVLKK32 pKa = 10.4 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 LKK37 pKa = 10.17 NRR39 pKa = 11.84 KK40 pKa = 8.78 VLSAA44 pKa = 4.17
Molecular weight: 5.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.434
IPC2_protein 11.14
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.427
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.149
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.052
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1418
0
1418
449836
37
3043
317.2
36.09
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.951 ± 0.068
1.063 ± 0.021
5.683 ± 0.053
7.784 ± 0.081
5.323 ± 0.068
6.046 ± 0.06
1.218 ± 0.022
9.851 ± 0.081
9.889 ± 0.087
9.223 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.473 ± 0.033
6.363 ± 0.056
2.49 ± 0.028
1.974 ± 0.028
3.344 ± 0.044
6.377 ± 0.048
4.682 ± 0.052
6.543 ± 0.059
0.594 ± 0.02
4.13 ± 0.049
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here