Arthrobacter phage KellEzio
Average proteome isoelectric point is 5.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 98 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A140G6F4|A0A140G6F4_9CAUD Uncharacterized protein OS=Arthrobacter phage KellEzio OX=1796995 GN=69 PE=4 SV=1
MM1 pKa = 8.29 DD2 pKa = 4.67 IGGKK6 pKa = 9.6 IKK8 pKa = 9.97 IDD10 pKa = 3.35 GRR12 pKa = 11.84 EE13 pKa = 4.12 TEE15 pKa = 4.84 FLLTDD20 pKa = 3.77 EE21 pKa = 5.02 GSYY24 pKa = 9.85 IQWGADD30 pKa = 2.81 TSTLGEE36 pKa = 4.09 RR37 pKa = 11.84 TDD39 pKa = 3.98 LLEE42 pKa = 4.57 KK43 pKa = 10.72 LGEE46 pKa = 4.53 AYY48 pKa = 10.68 VEE50 pKa = 4.0 WAQDD54 pKa = 3.85 NLCSEE59 pKa = 4.65 CKK61 pKa = 10.35 DD62 pKa = 3.23 RR63 pKa = 11.84 TLDD66 pKa = 4.54 DD67 pKa = 4.35 GEE69 pKa = 4.66 GFNGRR74 pKa = 11.84 CGNCADD80 pKa = 4.23 RR81 pKa = 11.84 AEE83 pKa = 4.62 KK84 pKa = 10.59 EE85 pKa = 4.22 GSNDD89 pKa = 4.48 DD90 pKa = 3.63 NDD92 pKa = 3.56
Molecular weight: 10.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.234
IPC2_protein 4.012
IPC_protein 3.961
Toseland 3.757
ProMoST 4.05
Dawson 3.935
Bjellqvist 4.139
Wikipedia 3.859
Rodwell 3.795
Grimsley 3.668
Solomon 3.935
Lehninger 3.884
Nozaki 4.062
DTASelect 4.253
Thurlkill 3.808
EMBOSS 3.872
Sillero 4.075
Patrickios 3.592
IPC_peptide 3.935
IPC2_peptide 4.062
IPC2.peptide.svr19 4.017
Protein with the highest isoelectric point:
>tr|A0A140G6D6|A0A140G6D6_9CAUD Uncharacterized protein OS=Arthrobacter phage KellEzio OX=1796995 GN=51 PE=4 SV=1
MM1 pKa = 7.12 GRR3 pKa = 11.84 RR4 pKa = 11.84 RR5 pKa = 11.84 KK6 pKa = 10.18 KK7 pKa = 9.44 YY8 pKa = 6.83 WAPMFNAKK16 pKa = 9.89 PPRR19 pKa = 11.84 QPVTQFTRR27 pKa = 11.84 GAKK30 pKa = 9.66 EE31 pKa = 3.48 GAGWVGRR38 pKa = 11.84 GVWGTIKK45 pKa = 10.73 LMWRR49 pKa = 11.84 IRR51 pKa = 3.47
Molecular weight: 6.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.255
IPC2_protein 10.701
IPC_protein 12.076
Toseland 12.237
ProMoST 12.735
Dawson 12.237
Bjellqvist 12.237
Wikipedia 12.705
Rodwell 12.018
Grimsley 12.281
Solomon 12.735
Lehninger 12.632
Nozaki 12.237
DTASelect 12.237
Thurlkill 12.237
EMBOSS 12.735
Sillero 12.237
Patrickios 11.769
IPC_peptide 12.735
IPC2_peptide 11.711
IPC2.peptide.svr19 9.096
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
98
0
98
18401
39
1704
187.8
20.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.923 ± 0.231
0.837 ± 0.129
6.706 ± 0.326
6.853 ± 0.452
3.049 ± 0.22
8.065 ± 0.403
2.038 ± 0.223
4.842 ± 0.25
4.94 ± 0.266
7.972 ± 0.298
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.603 ± 0.144
3.685 ± 0.243
5.049 ± 0.243
3.695 ± 0.232
5.646 ± 0.261
5.445 ± 0.373
6.418 ± 0.433
7.217 ± 0.296
2.451 ± 0.185
2.565 ± 0.149
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here