Turnip leaf roll virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Turncurtovirus

Average proteome isoelectric point is 7.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S3JNN5|A0A0S3JNN5_9GEMI Replication-associated protein OS=Turnip leaf roll virus OX=1766828 PE=3 SV=1
MM1 pKa = 7.42IWRR4 pKa = 11.84VNKK7 pKa = 9.7DD8 pKa = 3.46DD9 pKa = 3.47KK10 pKa = 10.79RR11 pKa = 11.84VRR13 pKa = 11.84AFRR16 pKa = 11.84YY17 pKa = 8.4KK18 pKa = 10.17KK19 pKa = 9.97RR20 pKa = 11.84RR21 pKa = 11.84FYY23 pKa = 11.51SLAEE27 pKa = 3.98YY28 pKa = 10.82LLAPLATHH36 pKa = 6.85LCKK39 pKa = 10.66GRR41 pKa = 11.84IFPDD45 pKa = 3.36DD46 pKa = 4.02KK47 pKa = 11.35YY48 pKa = 11.38LVPAYY53 pKa = 9.31TDD55 pKa = 3.2EE56 pKa = 5.01AYY58 pKa = 11.07SFLVFLKK65 pKa = 10.63EE66 pKa = 3.78EE67 pKa = 4.17TRR69 pKa = 11.84TPFQILKK76 pKa = 10.45DD77 pKa = 3.52EE78 pKa = 4.46WNVEE82 pKa = 4.15FKK84 pKa = 10.63EE85 pKa = 4.18IEE87 pKa = 4.16VEE89 pKa = 4.23LSRR92 pKa = 11.84LRR94 pKa = 11.84SGPKK98 pKa = 9.86DD99 pKa = 3.33AGEE102 pKa = 3.84EE103 pKa = 4.05AGDD106 pKa = 3.99KK107 pKa = 10.46EE108 pKa = 4.76GSLSIPISVCGEE120 pKa = 3.9TSLEE124 pKa = 3.81EE125 pKa = 4.75DD126 pKa = 4.0VYY128 pKa = 11.74EE129 pKa = 4.78DD130 pKa = 3.45

Molecular weight: 15.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S3JNV6|A0A0S3JNV6_9GEMI Capsid protein OS=Turnip leaf roll virus OX=1766828 PE=3 SV=1
MM1 pKa = 7.45SGTWSLKK8 pKa = 8.4RR9 pKa = 11.84SRR11 pKa = 11.84WNSPGYY17 pKa = 10.47DD18 pKa = 3.8LAPKK22 pKa = 9.63TPAKK26 pKa = 10.23RR27 pKa = 11.84PAIRR31 pKa = 11.84RR32 pKa = 11.84ALFQSPSQYY41 pKa = 9.5VARR44 pKa = 11.84RR45 pKa = 11.84PWRR48 pKa = 11.84RR49 pKa = 11.84TFTRR53 pKa = 11.84TNKK56 pKa = 9.87GVSKK60 pKa = 10.61KK61 pKa = 10.1KK62 pKa = 10.65LKK64 pKa = 10.85ASDD67 pKa = 3.5YY68 pKa = 10.58QIYY71 pKa = 9.38EE72 pKa = 4.14DD73 pKa = 5.59RR74 pKa = 11.84VGGDD78 pKa = 3.3NGWTVTSTGDD88 pKa = 3.27CTMLNNYY95 pKa = 8.7VRR97 pKa = 11.84GIGRR101 pKa = 11.84DD102 pKa = 3.55QRR104 pKa = 11.84DD105 pKa = 3.31STLTKK110 pKa = 8.64TVHH113 pKa = 4.5MHH115 pKa = 5.93FSGVLMANDD124 pKa = 4.5AFWEE128 pKa = 4.53APNYY132 pKa = 6.13MTMYY136 pKa = 10.36SWIILDD142 pKa = 3.98NDD144 pKa = 4.09PGGSFPKK151 pKa = 10.22PADD154 pKa = 3.33IFDD157 pKa = 3.9MTDD160 pKa = 2.63KK161 pKa = 10.75DD162 pKa = 4.39FPSMYY167 pKa = 10.11EE168 pKa = 3.66VAEE171 pKa = 4.23SVKK174 pKa = 10.27PRR176 pKa = 11.84FIVKK180 pKa = 10.11RR181 pKa = 11.84KK182 pKa = 6.14TRR184 pKa = 11.84HH185 pKa = 4.59YY186 pKa = 10.76LRR188 pKa = 11.84SCGVAFGEE196 pKa = 4.26KK197 pKa = 9.6QNYY200 pKa = 7.5KK201 pKa = 10.67APTLGPIKK209 pKa = 10.41KK210 pKa = 8.76PISMVFRR217 pKa = 11.84NMWQMTEE224 pKa = 3.78WKK226 pKa = 8.6DD227 pKa = 3.55TAGGKK232 pKa = 10.06YY233 pKa = 10.11EE234 pKa = 4.17DD235 pKa = 4.21LKK237 pKa = 11.27KK238 pKa = 10.78GALLFVCMSDD248 pKa = 3.17NKK250 pKa = 10.61ASQFSFSLRR259 pKa = 11.84GKK261 pKa = 8.49WKK263 pKa = 9.22MYY265 pKa = 9.86FINRR269 pKa = 11.84DD270 pKa = 3.13RR271 pKa = 11.84FQQ273 pKa = 5.04

Molecular weight: 31.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1140

85

371

190.0

21.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.263 ± 0.962

1.228 ± 0.155

5.263 ± 0.65

6.053 ± 1.087

4.737 ± 0.503

5.702 ± 0.369

3.333 ± 0.811

6.404 ± 0.984

7.018 ± 0.736

7.719 ± 0.84

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.281 ± 0.885

5.088 ± 0.666

5.965 ± 0.888

3.86 ± 0.534

6.228 ± 0.825

7.193 ± 0.679

6.404 ± 0.858

4.561 ± 0.554

1.93 ± 0.383

3.772 ± 0.355
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski