Streptococcus satellite phage Javan607
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 18 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZRT5|A0A4D5ZRT5_9VIRU Mobile element protein OS=Streptococcus satellite phage Javan607 OX=2558776 GN=JavanS607_0002 PE=4 SV=1
MM1 pKa = 7.26 LAKK4 pKa = 10.28 HH5 pKa = 6.4 AGADD9 pKa = 3.57 NGLLRR14 pKa = 11.84 YY15 pKa = 7.65 EE16 pKa = 4.02 TDD18 pKa = 3.28 KK19 pKa = 11.26 FLEE22 pKa = 4.53 TVFDD26 pKa = 3.75 MVEE29 pKa = 3.95 IYY31 pKa = 10.61 SDD33 pKa = 3.42 EE34 pKa = 4.4 LDD36 pKa = 3.99 RR37 pKa = 11.84 VACYY41 pKa = 10.43 LLEE44 pKa = 4.18 YY45 pKa = 10.55 DD46 pKa = 5.33 NPDD49 pKa = 3.18 EE50 pKa = 4.07 LRR52 pKa = 11.84 AYY54 pKa = 9.86 EE55 pKa = 4.09 EE56 pKa = 4.38 EE57 pKa = 4.45 EE58 pKa = 3.99 KK59 pKa = 11.53 GEE61 pKa = 4.06
Molecular weight: 7.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.058
IPC2_protein 4.101
IPC_protein 3.999
Toseland 3.821
ProMoST 4.075
Dawson 3.961
Bjellqvist 4.177
Wikipedia 3.846
Rodwell 3.834
Grimsley 3.745
Solomon 3.948
Lehninger 3.897
Nozaki 4.075
DTASelect 4.215
Thurlkill 3.859
EMBOSS 3.859
Sillero 4.113
Patrickios 2.943
IPC_peptide 3.948
IPC2_peptide 4.088
IPC2.peptide.svr19 4.03
Protein with the highest isoelectric point:
>tr|A0A4D5ZS98|A0A4D5ZS98_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan607 OX=2558776 GN=JavanS607_0013 PE=4 SV=1
MM1 pKa = 7.29 WVEE4 pKa = 4.31 DD5 pKa = 4.38 LPNGKK10 pKa = 9.48 YY11 pKa = 9.93 KK12 pKa = 10.56 YY13 pKa = 9.82 CEE15 pKa = 4.17 RR16 pKa = 11.84 YY17 pKa = 8.24 TDD19 pKa = 3.43 TKK21 pKa = 10.99 GKK23 pKa = 9.45 IRR25 pKa = 11.84 KK26 pKa = 8.72 KK27 pKa = 10.88 VSVTLDD33 pKa = 3.45 KK34 pKa = 11.37 NSSRR38 pKa = 11.84 AQNEE42 pKa = 3.91 ASRR45 pKa = 11.84 LLYY48 pKa = 10.78 NKK50 pKa = 9.55 IDD52 pKa = 3.55 AKK54 pKa = 10.9 LEE56 pKa = 4.13 KK57 pKa = 10.16 KK58 pKa = 9.95 NKK60 pKa = 7.83 KK61 pKa = 10.26 LKK63 pKa = 9.29 MSKK66 pKa = 8.95 TKK68 pKa = 10.74
Molecular weight: 8.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.065
IPC2_protein 9.414
IPC_protein 9.311
Toseland 10.306
ProMoST 9.853
Dawson 10.409
Bjellqvist 9.999
Wikipedia 10.526
Rodwell 11.169
Grimsley 10.452
Solomon 10.438
Lehninger 10.423
Nozaki 10.262
DTASelect 9.999
Thurlkill 10.292
EMBOSS 10.672
Sillero 10.321
Patrickios 10.906
IPC_peptide 10.438
IPC2_peptide 8.448
IPC2.peptide.svr19 8.409
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
18
0
18
2803
38
501
155.7
17.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.494 ± 0.406
0.535 ± 0.131
6.35 ± 0.619
7.563 ± 0.78
4.031 ± 0.482
5.102 ± 0.408
1.641 ± 0.419
6.493 ± 0.654
9.561 ± 0.835
8.669 ± 0.552
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.319 ± 0.281
6.35 ± 0.487
3.354 ± 0.575
3.461 ± 0.444
3.568 ± 0.437
6.564 ± 0.668
6.386 ± 0.753
6.208 ± 0.339
1.142 ± 0.162
5.209 ± 0.476
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here