Mycobacterium phage Cracklewink

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 135 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y6EPC1|A0A4Y6EPC1_9CAUD Uncharacterized protein OS=Mycobacterium phage Cracklewink OX=2588510 GN=64 PE=4 SV=1
MM1 pKa = 7.21ATITVWTLIIVGDD14 pKa = 4.15NEE16 pKa = 4.78DD17 pKa = 3.63STSVSLHH24 pKa = 6.04PSEE27 pKa = 5.03AAALGHH33 pKa = 6.76LRR35 pKa = 11.84EE36 pKa = 4.98LYY38 pKa = 10.24DD39 pKa = 3.34IPEE42 pKa = 4.1WVEE45 pKa = 4.06DD46 pKa = 3.99VVDD49 pKa = 4.3AVNNDD54 pKa = 3.54PVITNGEE61 pKa = 3.97EE62 pKa = 3.76VRR64 pKa = 11.84FVMLDD69 pKa = 3.4SHH71 pKa = 7.11DD72 pKa = 4.31HH73 pKa = 5.25YY74 pKa = 11.76CC75 pKa = 4.84

Molecular weight: 8.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y6EVD3|A0A4Y6EVD3_9CAUD Uncharacterized protein OS=Mycobacterium phage Cracklewink OX=2588510 GN=67 PE=4 SV=1
MM1 pKa = 6.73TTACSKK7 pKa = 10.21CRR9 pKa = 11.84QRR11 pKa = 11.84PATGGRR17 pKa = 11.84GLCEE21 pKa = 3.84NCWAVNRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.45LMAYY34 pKa = 9.32GRR36 pKa = 11.84WDD38 pKa = 3.92PDD40 pKa = 3.34QLDD43 pKa = 3.79PTPTRR48 pKa = 11.84QHH50 pKa = 6.38LAALQQWGMGTRR62 pKa = 11.84RR63 pKa = 11.84LQEE66 pKa = 3.75LTGLSRR72 pKa = 11.84PTLQGIPEE80 pKa = 4.43AAWVTRR86 pKa = 11.84KK87 pKa = 8.26TEE89 pKa = 3.85AALLAVPIPTTVFDD103 pKa = 4.36PVLADD108 pKa = 3.58GTQISSVGSRR118 pKa = 11.84RR119 pKa = 11.84RR120 pKa = 11.84LCALSAIGWSGEE132 pKa = 3.72ALAEE136 pKa = 3.84QLGVMRR142 pKa = 11.84HH143 pKa = 4.95RR144 pKa = 11.84VQAITSGRR152 pKa = 11.84QPKK155 pKa = 8.31VTVARR160 pKa = 11.84ARR162 pKa = 11.84DD163 pKa = 3.36IAHH166 pKa = 7.24LFNRR170 pKa = 11.84LQLTEE175 pKa = 4.24GPSRR179 pKa = 11.84KK180 pKa = 9.45ARR182 pKa = 11.84RR183 pKa = 11.84VAQLKK188 pKa = 9.25GWPLPLQWDD197 pKa = 4.19EE198 pKa = 5.81DD199 pKa = 4.14RR200 pKa = 11.84LDD202 pKa = 4.12DD203 pKa = 5.53PRR205 pKa = 11.84AKK207 pKa = 9.23PHH209 pKa = 6.47KK210 pKa = 9.92RR211 pKa = 11.84RR212 pKa = 11.84GPKK215 pKa = 10.11GSAEE219 pKa = 3.89DD220 pKa = 4.8RR221 pKa = 11.84ITEE224 pKa = 4.23LYY226 pKa = 10.37EE227 pKa = 3.64LGIRR231 pKa = 11.84DD232 pKa = 4.1TEE234 pKa = 4.18AMAEE238 pKa = 4.0RR239 pKa = 11.84LGIKK243 pKa = 9.62PEE245 pKa = 4.13SVVRR249 pKa = 11.84QINRR253 pKa = 11.84MRR255 pKa = 11.84AASS258 pKa = 3.37

Molecular weight: 28.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

135

0

135

24573

29

1561

182.0

19.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.203 ± 0.405

1.363 ± 0.152

6.825 ± 0.215

5.6 ± 0.251

2.36 ± 0.12

8.733 ± 0.365

2.568 ± 0.179

3.581 ± 0.173

3.064 ± 0.186

7.895 ± 0.263

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.454 ± 0.12

3.007 ± 0.151

6.422 ± 0.274

4.082 ± 0.158

7.227 ± 0.315

4.806 ± 0.121

6.54 ± 0.212

7.622 ± 0.233

2.141 ± 0.151

2.507 ± 0.105
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski