Spirosoma agri

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cytophagaceae; Spirosoma

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5746 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M0II99|A0A6M0II99_9BACT Uncharacterized protein OS=Spirosoma agri OX=1987381 GN=GK091_13790 PE=4 SV=1
MM1 pKa = 7.65APLPSTDD8 pKa = 3.07IEE10 pKa = 4.26LGKK13 pKa = 9.7WYY15 pKa = 8.48PATLIPPDD23 pKa = 3.43LHH25 pKa = 7.02EE26 pKa = 4.42EE27 pKa = 4.13QFGNDD32 pKa = 2.94YY33 pKa = 11.77SMILGIDD40 pKa = 3.4GHH42 pKa = 7.06GSVPGCYY49 pKa = 9.47FLQKK53 pKa = 10.99GMFYY57 pKa = 9.13WHH59 pKa = 7.26CEE61 pKa = 3.68RR62 pKa = 11.84YY63 pKa = 10.5GDD65 pKa = 4.69FDD67 pKa = 5.15DD68 pKa = 4.74EE69 pKa = 5.83AISGWTRR76 pKa = 11.84AQEE79 pKa = 3.85INNNIEE85 pKa = 3.62WEE87 pKa = 4.08TDD89 pKa = 3.25VAKK92 pKa = 10.71DD93 pKa = 3.35VSGWMIIADD102 pKa = 3.62VPKK105 pKa = 10.5DD106 pKa = 3.61FEE108 pKa = 4.78TIQTDD113 pKa = 3.4AVNSFIGGEE122 pKa = 3.98PSNDD126 pKa = 2.92EE127 pKa = 5.55DD128 pKa = 6.33DD129 pKa = 6.68FDD131 pKa = 5.84DD132 pKa = 5.33QDD134 pKa = 4.43DD135 pKa = 4.35DD136 pKa = 5.01PYY138 pKa = 11.3HH139 pKa = 6.85HH140 pKa = 7.36SPDD143 pKa = 3.44TCC145 pKa = 4.88

Molecular weight: 16.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M0IKY1|A0A6M0IKY1_9BACT Malto-oligosyltrehalose synthase OS=Spirosoma agri OX=1987381 GN=treY PE=4 SV=1
MM1 pKa = 7.5RR2 pKa = 11.84SSVRR6 pKa = 11.84MVLLTRR12 pKa = 11.84MVLLTRR18 pKa = 11.84MVLLTRR24 pKa = 11.84MVLLTRR30 pKa = 11.84MVLLTRR36 pKa = 11.84MRR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.96GLRR43 pKa = 11.84LYY45 pKa = 9.43TINKK49 pKa = 7.63HH50 pKa = 5.72RR51 pKa = 11.84RR52 pKa = 11.84IKK54 pKa = 10.91SGGKK58 pKa = 8.39EE59 pKa = 3.64

Molecular weight: 7.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5746

0

5746

2038134

26

3024

354.7

39.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.375 ± 0.035

0.72 ± 0.009

5.421 ± 0.024

4.939 ± 0.031

4.413 ± 0.022

7.282 ± 0.028

1.793 ± 0.018

5.794 ± 0.024

4.916 ± 0.033

10.012 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.066 ± 0.014

4.686 ± 0.03

4.594 ± 0.021

4.496 ± 0.021

5.296 ± 0.026

6.373 ± 0.03

6.782 ± 0.041

6.952 ± 0.025

1.289 ± 0.012

3.8 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski