Helicobacter bizzozeronii (strain CIII-1)
Average proteome isoelectric point is 7.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1959 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F8KQ95|F8KQ95_HELBC Thiamine-monophosphate kinase OS=Helicobacter bizzozeronii (strain CIII-1) OX=1002804 GN=thiL PE=3 SV=1
MM1 pKa = 6.97 QVKK4 pKa = 8.19 TLDD7 pKa = 4.06 PLSQQALEE15 pKa = 4.44 EE16 pKa = 4.62 IGLDD20 pKa = 3.11 WHH22 pKa = 6.89 TDD24 pKa = 3.4 TDD26 pKa = 3.58 NSPYY30 pKa = 10.09 ISQDD34 pKa = 3.25 LVIVSQSEE42 pKa = 3.36 ADD44 pKa = 3.78 AYY46 pKa = 11.41 YY47 pKa = 9.72 EE48 pKa = 4.27 ACNEE52 pKa = 3.95 LYY54 pKa = 11.13 EE55 pKa = 4.45 MFVEE59 pKa = 5.0 TAQEE63 pKa = 4.11 IIEE66 pKa = 4.07 HH67 pKa = 6.54 DD68 pKa = 3.71 RR69 pKa = 11.84 FFEE72 pKa = 4.29 LDD74 pKa = 3.37 IPNSIVPLIKK84 pKa = 10.42 QSWEE88 pKa = 4.24 NEE90 pKa = 4.54 VHH92 pKa = 3.57 WHH94 pKa = 5.59 IYY96 pKa = 9.97 GRR98 pKa = 11.84 FDD100 pKa = 4.57 LAGGLDD106 pKa = 3.67 GKK108 pKa = 9.49 PIKK111 pKa = 10.44 LLEE114 pKa = 4.39 FNADD118 pKa = 3.85 TPTMLYY124 pKa = 10.26 EE125 pKa = 4.22 SAVDD129 pKa = 3.96 AMGVTQIQWLL139 pKa = 3.77
Molecular weight: 15.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.791
IPC2_protein 3.986
IPC_protein 3.935
Toseland 3.757
ProMoST 4.075
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.808
Rodwell 3.77
Grimsley 3.668
Solomon 3.897
Lehninger 3.859
Nozaki 4.024
DTASelect 4.202
Thurlkill 3.795
EMBOSS 3.821
Sillero 4.062
Patrickios 1.901
IPC_peptide 3.897
IPC2_peptide 4.037
IPC2.peptide.svr19 3.929
Protein with the highest isoelectric point:
>tr|F8KQQ7|F8KQQ7_HELBC Putative mechanosensitive ion channel OS=Helicobacter bizzozeronii (strain CIII-1) OX=1002804 GN=HBZC1_00750 PE=4 SV=1
MM1 pKa = 7.62 SIFMAGSPSWRR12 pKa = 11.84 VTHH15 pKa = 6.93 IPIPRR20 pKa = 11.84 LKK22 pKa = 9.94 AHH24 pKa = 6.83 RR25 pKa = 11.84 PIAQRR30 pKa = 11.84 RR31 pKa = 11.84 AQHH34 pKa = 4.94 SHH36 pKa = 6.19 ALFF39 pKa = 4.13
Molecular weight: 4.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.393
IPC2_protein 10.833
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.193
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.974
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.127
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1959
0
1959
548772
37
2923
280.1
31.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.896 ± 0.053
1.265 ± 0.028
4.749 ± 0.039
5.836 ± 0.065
4.886 ± 0.051
6.529 ± 0.064
2.728 ± 0.031
6.467 ± 0.054
7.1 ± 0.051
11.408 ± 0.07
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.354 ± 0.025
4.376 ± 0.053
4.15 ± 0.044
4.981 ± 0.056
3.911 ± 0.035
6.294 ± 0.045
4.593 ± 0.045
5.978 ± 0.052
0.879 ± 0.019
3.613 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here