Salmonella phage vB_SenS_AG11
Average proteome isoelectric point is 6.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S4SGH6|S4SGH6_9CAUD Uncharacterized protein OS=Salmonella phage vB_SenS_AG11 OX=1211279 GN=AG11_56 PE=4 SV=1
MM1 pKa = 7.41 EE2 pKa = 5.55 LVHH5 pKa = 7.33 DD6 pKa = 4.6 IDD8 pKa = 4.86 DD9 pKa = 4.24 ASEE12 pKa = 4.06 AMALTANGAHH22 pKa = 6.62 EE23 pKa = 4.48 EE24 pKa = 4.35 YY25 pKa = 9.98 IGASMLPGIQGLYY38 pKa = 10.07
Molecular weight: 4.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.842
IPC2_protein 4.012
IPC_protein 3.77
Toseland 3.63
ProMoST 3.973
Dawson 3.77
Bjellqvist 3.948
Wikipedia 3.719
Rodwell 3.643
Grimsley 3.567
Solomon 3.719
Lehninger 3.681
Nozaki 3.935
DTASelect 4.037
Thurlkill 3.706
EMBOSS 3.732
Sillero 3.91
Patrickios 0.477
IPC_peptide 3.719
IPC2_peptide 3.872
IPC2.peptide.svr19 3.819
Protein with the highest isoelectric point:
>tr|S4SGS1|S4SGS1_9CAUD Putative holin-like class I OS=Salmonella phage vB_SenS_AG11 OX=1211279 GN=AG11_57 PE=4 SV=1
MM1 pKa = 7.61 KK2 pKa = 10.34 PNDD5 pKa = 3.53 LVTWAGRR12 pKa = 11.84 NGEE15 pKa = 4.18 TRR17 pKa = 11.84 HH18 pKa = 5.66 GKK20 pKa = 8.01 VTALQGIYY28 pKa = 10.7 ARR30 pKa = 11.84 VEE32 pKa = 3.33 WWRR35 pKa = 11.84 AHH37 pKa = 5.21 AKK39 pKa = 9.39 KK40 pKa = 9.94 PRR42 pKa = 11.84 YY43 pKa = 8.21 LTVRR47 pKa = 11.84 RR48 pKa = 11.84 DD49 pKa = 3.49 KK50 pKa = 11.41 LIVKK54 pKa = 9.6
Molecular weight: 6.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.286
IPC2_protein 10.043
IPC_protein 11.008
Toseland 11.199
ProMoST 11.228
Dawson 11.242
Bjellqvist 11.038
Wikipedia 11.535
Rodwell 11.447
Grimsley 11.286
Solomon 11.506
Lehninger 11.447
Nozaki 11.169
DTASelect 11.038
Thurlkill 11.184
EMBOSS 11.623
Sillero 11.199
Patrickios 11.199
IPC_peptide 11.506
IPC2_peptide 10.101
IPC2.peptide.svr19 8.485
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
66
0
66
13005
36
1032
197.0
21.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.765 ± 0.679
1.015 ± 0.151
6.136 ± 0.26
6.636 ± 0.372
3.683 ± 0.181
7.712 ± 0.32
1.676 ± 0.196
4.852 ± 0.194
5.913 ± 0.331
7.782 ± 0.286
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.722 ± 0.181
4.275 ± 0.211
3.945 ± 0.228
3.783 ± 0.391
5.698 ± 0.207
5.821 ± 0.25
6.459 ± 0.327
7.282 ± 0.334
1.453 ± 0.167
3.391 ± 0.199
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here