Ruminococcus sp. CAG:579

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Ruminococcus; environmental samples

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2155 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6CKK3|R6CKK3_9FIRM ACT domain protein OS=Ruminococcus sp. CAG:579 OX=1262963 GN=BN718_01721 PE=4 SV=1
MM1 pKa = 8.02RR2 pKa = 11.84STKK5 pKa = 10.24SAALICAAVLVLMSMSACSKK25 pKa = 11.01SEE27 pKa = 3.86TAKK30 pKa = 10.24TNSSNADD37 pKa = 3.14SSSAKK42 pKa = 9.65STDD45 pKa = 2.97KK46 pKa = 11.32KK47 pKa = 10.84LDD49 pKa = 3.45VYY51 pKa = 10.87LSSDD55 pKa = 3.27GTPYY59 pKa = 11.15YY60 pKa = 10.66LADD63 pKa = 5.58DD64 pKa = 4.03GTKK67 pKa = 9.75MNLYY71 pKa = 10.43AYY73 pKa = 10.15ADD75 pKa = 4.03EE76 pKa = 6.13DD77 pKa = 4.6DD78 pKa = 4.87DD79 pKa = 4.18TAEE82 pKa = 4.96DD83 pKa = 3.87EE84 pKa = 4.89ASANYY89 pKa = 9.94IYY91 pKa = 10.83DD92 pKa = 3.74KK93 pKa = 11.35YY94 pKa = 11.34DD95 pKa = 3.7ADD97 pKa = 3.85GLKK100 pKa = 10.25FDD102 pKa = 5.11IPEE105 pKa = 3.75GWFADD110 pKa = 3.86TSFGSPMLLQQADD123 pKa = 3.63SDD125 pKa = 4.83DD126 pKa = 4.4EE127 pKa = 4.42INYY130 pKa = 10.03DD131 pKa = 3.54EE132 pKa = 5.51SIAIVDD138 pKa = 3.87TEE140 pKa = 4.45NVFDD144 pKa = 4.13SDD146 pKa = 3.4NGKK149 pKa = 8.21VTKK152 pKa = 9.34KK153 pKa = 8.3TVKK156 pKa = 10.32SYY158 pKa = 10.96FDD160 pKa = 3.56NYY162 pKa = 10.74LDD164 pKa = 3.36YY165 pKa = 11.17GYY167 pKa = 9.06YY168 pKa = 9.07TDD170 pKa = 4.46YY171 pKa = 10.83KK172 pKa = 10.36ISEE175 pKa = 4.32TGSMKK180 pKa = 10.42VSGVDD185 pKa = 3.04ANYY188 pKa = 10.56YY189 pKa = 10.71DD190 pKa = 4.54VITTISSALTNTDD203 pKa = 2.75SDD205 pKa = 3.67EE206 pKa = 4.33DD207 pKa = 4.12AEE209 pKa = 4.25PVVCKK214 pKa = 9.66TRR216 pKa = 11.84YY217 pKa = 9.75IITDD221 pKa = 3.69EE222 pKa = 4.27DD223 pKa = 4.06TSHH226 pKa = 7.48CMILSALNNDD236 pKa = 3.33EE237 pKa = 4.2SFGRR241 pKa = 11.84VVEE244 pKa = 4.93AYY246 pKa = 9.61EE247 pKa = 4.12KK248 pKa = 10.31MSGSLEE254 pKa = 4.18LPSS257 pKa = 3.81

Molecular weight: 28.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6CNF1|R6CNF1_9FIRM Uncharacterized protein OS=Ruminococcus sp. CAG:579 OX=1262963 GN=BN718_00350 PE=4 SV=1
MM1 pKa = 7.67SNRR4 pKa = 11.84FCTGCGAPLTPDD16 pKa = 3.1MKK18 pKa = 10.47ICPRR22 pKa = 11.84CGKK25 pKa = 9.52VAAVSARR32 pKa = 11.84PRR34 pKa = 11.84PQQRR38 pKa = 11.84APRR41 pKa = 11.84PQQPVRR47 pKa = 11.84NVRR50 pKa = 11.84TVRR53 pKa = 11.84TGQTVQNGQTVRR65 pKa = 11.84NSQPQARR72 pKa = 11.84RR73 pKa = 11.84PQTAPQRR80 pKa = 11.84RR81 pKa = 11.84PAPMPRR87 pKa = 11.84PSPAPEE93 pKa = 3.87RR94 pKa = 11.84QEE96 pKa = 3.86RR97 pKa = 11.84APKK100 pKa = 10.0KK101 pKa = 8.81RR102 pKa = 11.84SKK104 pKa = 8.61KK105 pKa = 10.34ARR107 pKa = 11.84MIFHH111 pKa = 7.6AATAAVVLVGLYY123 pKa = 9.11FAIFFVQIFRR133 pKa = 11.84VKK135 pKa = 10.29ISTYY139 pKa = 10.06DD140 pKa = 3.21FKK142 pKa = 11.28TEE144 pKa = 3.66IKK146 pKa = 10.6LEE148 pKa = 4.08SSNYY152 pKa = 7.32GQAMEE157 pKa = 5.14SYY159 pKa = 9.88FEE161 pKa = 4.07SGKK164 pKa = 10.08WSVNPLTGTCTYY176 pKa = 10.63TGTTKK181 pKa = 10.6HH182 pKa = 5.91GDD184 pKa = 3.34EE185 pKa = 4.35YY186 pKa = 10.91EE187 pKa = 4.09IVYY190 pKa = 7.75TARR193 pKa = 11.84LKK195 pKa = 11.02VDD197 pKa = 3.46VKK199 pKa = 11.08NISINGKK206 pKa = 7.51DD207 pKa = 3.36VRR209 pKa = 11.84ADD211 pKa = 4.33RR212 pKa = 11.84IEE214 pKa = 4.07STLMGMFII222 pKa = 4.87

Molecular weight: 24.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2155

0

2155

683710

29

3445

317.3

35.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.84 ± 0.063

1.718 ± 0.027

6.194 ± 0.046

7.238 ± 0.056

4.076 ± 0.037

7.112 ± 0.051

1.553 ± 0.022

6.954 ± 0.04

6.924 ± 0.048

8.245 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.898 ± 0.026

4.397 ± 0.045

3.312 ± 0.033

2.754 ± 0.026

4.369 ± 0.052

6.411 ± 0.053

5.559 ± 0.051

6.523 ± 0.045

0.803 ± 0.02

4.106 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski