Amino acid dipepetide frequency for Ruminococcus sp. CAG:579

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.269AlaAla: 11.269 ± 0.188	1.294AlaCys: 1.294 ± 0.047	6.766AlaAsp: 6.766 ± 0.12	8.194AlaGlu: 8.194 ± 0.158	3.194AlaPhe: 3.194 ± 0.082	6.098AlaGly: 6.098 ± 0.113	1.306AlaHis: 1.306 ± 0.042	4.901AlaIle: 4.901 ± 0.086	5.613AlaLys: 5.613 ± 0.101	7.673AlaLeu: 7.673 ± 0.139	2.546AlaMet: 2.546 ± 0.072	2.99AlaAsn: 2.99 ± 0.073	2.445AlaPro: 2.445 ± 0.073	2.769AlaGln: 2.769 ± 0.071	3.365AlaArg: 3.365 ± 0.087	4.125AlaSer: 4.125 ± 0.076	3.055AlaThr: 3.055 ± 0.079	7.278AlaVal: 7.278 ± 0.106	0.513AlaTrp: 0.513 ± 0.027	2.988AlaTyr: 2.988 ± 0.062	0.013AlaXaa: 0.013 ± 0.004
Cys	1.625CysAla: 1.625 ± 0.058	0.383CysCys: 0.383 ± 0.025	1.031CysAsp: 1.031 ± 0.046	1.353CysGlu: 1.353 ± 0.046	0.646CysPhe: 0.646 ± 0.031	2.007CysGly: 2.007 ± 0.068	0.241CysHis: 0.241 ± 0.018	1.071CysIle: 1.071 ± 0.041	0.904CysLys: 0.904 ± 0.037	1.046CysLeu: 1.046 ± 0.04	0.398CysMet: 0.398 ± 0.025	0.604CysAsn: 0.604 ± 0.032	0.714CysPro: 0.714 ± 0.043	0.243CysGln: 0.243 ± 0.018	0.867CysArg: 0.867 ± 0.037	1.15CysSer: 1.15 ± 0.039	1.002CysThr: 1.002 ± 0.041	1.172CysVal: 1.172 ± 0.042	0.123CysTrp: 0.123 ± 0.013	0.6CysTyr: 0.6 ± 0.03	0.0CysXaa: 0.0 ± 0.0
Asp	4.009AspAla: 4.009 ± 0.077	1.022AspCys: 1.022 ± 0.039	3.841AspAsp: 3.841 ± 0.105	5.32AspGlu: 5.32 ± 0.096	2.94AspPhe: 2.94 ± 0.069	5.037AspGly: 5.037 ± 0.105	0.832AspHis: 0.832 ± 0.04	4.949AspIle: 4.949 ± 0.097	4.521AspLys: 4.521 ± 0.097	4.601AspLeu: 4.601 ± 0.086	2.034AspMet: 2.034 ± 0.055	2.756AspAsn: 2.756 ± 0.075	2.245AspPro: 2.245 ± 0.066	1.024AspGln: 1.024 ± 0.04	2.238AspArg: 2.238 ± 0.055	3.851AspSer: 3.851 ± 0.081	3.466AspThr: 3.466 ± 0.082	3.752AspVal: 3.752 ± 0.077	0.537AspTrp: 0.537 ± 0.033	2.963AspTyr: 2.963 ± 0.072	0.004AspXaa: 0.004 ± 0.002
Glu	5.207GluAla: 5.207 ± 0.118	1.059GluCys: 1.059 ± 0.042	3.82GluAsp: 3.82 ± 0.08	5.324GluGlu: 5.324 ± 0.12	2.503GluPhe: 2.503 ± 0.053	4.284GluGly: 4.284 ± 0.083	1.236GluHis: 1.236 ± 0.048	5.363GluIle: 5.363 ± 0.101	6.44GluLys: 6.44 ± 0.125	6.75GluLeu: 6.75 ± 0.109	2.318GluMet: 2.318 ± 0.062	4.481GluAsn: 4.481 ± 0.097	2.144GluPro: 2.144 ± 0.062	2.896GluGln: 2.896 ± 0.075	3.523GluArg: 3.523 ± 0.09	3.987GluSer: 3.987 ± 0.094	3.301GluThr: 3.301 ± 0.074	3.972GluVal: 3.972 ± 0.082	0.554GluTrp: 0.554 ± 0.028	3.208GluTyr: 3.208 ± 0.074	0.007GluXaa: 0.007 ± 0.003
Phe	3.662PheAla: 3.662 ± 0.079	0.802PheCys: 0.802 ± 0.038	3.086PheAsp: 3.086 ± 0.07	2.956PheGlu: 2.956 ± 0.062	1.79PhePhe: 1.79 ± 0.065	3.121PheGly: 3.121 ± 0.074	0.6PheHis: 0.6 ± 0.027	2.741PheIle: 2.741 ± 0.066	2.406PheLys: 2.406 ± 0.059	2.835PheLeu: 2.835 ± 0.064	1.176PheMet: 1.176 ± 0.042	1.587PheAsn: 1.587 ± 0.054	1.164PhePro: 1.164 ± 0.037	0.845PheGln: 0.845 ± 0.033	1.581PheArg: 1.581 ± 0.048	3.153PheSer: 3.153 ± 0.073	2.662PheThr: 2.662 ± 0.054	2.557PheVal: 2.557 ± 0.06	0.32PheTrp: 0.32 ± 0.021	1.711PheTyr: 1.711 ± 0.048	0.001PheXaa: 0.001 ± 0.001
Gly	5.752GlyAla: 5.752 ± 0.108	1.391GlyCys: 1.391 ± 0.051	4.154GlyAsp: 4.154 ± 0.088	5.398GlyGlu: 5.398 ± 0.105	3.172GlyPhe: 3.172 ± 0.071	5.796GlyGly: 5.796 ± 0.139	1.068GlyHis: 1.068 ± 0.049	5.02GlyIle: 5.02 ± 0.092	5.485GlyLys: 5.485 ± 0.093	5.627GlyLeu: 5.627 ± 0.09	2.168GlyMet: 2.168 ± 0.06	2.709GlyAsn: 2.709 ± 0.079	0.905GlyPro: 0.905 ± 0.044	1.583GlyGln: 1.583 ± 0.051	3.174GlyArg: 3.174 ± 0.079	4.509GlySer: 4.509 ± 0.073	4.019GlyThr: 4.019 ± 0.097	5.379GlyVal: 5.379 ± 0.087	0.704GlyTrp: 0.704 ± 0.039	3.093GlyTyr: 3.093 ± 0.075	0.012GlyXaa: 0.012 ± 0.004
His	1.125HisAla: 1.125 ± 0.043	0.338HisCys: 0.338 ± 0.019	0.911HisAsp: 0.911 ± 0.038	1.011HisGlu: 1.011 ± 0.033	0.74HisPhe: 0.74 ± 0.031	1.318HisGly: 1.318 ± 0.055	0.306HisHis: 0.306 ± 0.021	1.173HisIle: 1.173 ± 0.048	1.003HisLys: 1.003 ± 0.039	1.053HisLeu: 1.053 ± 0.04	0.429HisMet: 0.429 ± 0.026	0.67HisAsn: 0.67 ± 0.03	0.715HisPro: 0.715 ± 0.032	0.352HisGln: 0.352 ± 0.022	0.821HisArg: 0.821 ± 0.037	1.014HisSer: 1.014 ± 0.037	1.0HisThr: 1.0 ± 0.043	0.714HisVal: 0.714 ± 0.032	0.148HisTrp: 0.148 ± 0.015	0.693HisTyr: 0.693 ± 0.031	0.0HisXaa: 0.0 ± 0.0
Ile	6.117IleAla: 6.117 ± 0.11	1.506IleCys: 1.506 ± 0.051	4.419IleAsp: 4.419 ± 0.077	4.65IleGlu: 4.65 ± 0.094	3.051IlePhe: 3.051 ± 0.071	4.644IleGly: 4.644 ± 0.082	1.012IleHis: 1.012 ± 0.041	4.708IleIle: 4.708 ± 0.101	4.443IleLys: 4.443 ± 0.088	4.939IleLeu: 4.939 ± 0.086	2.138IleMet: 2.138 ± 0.06	3.169IleAsn: 3.169 ± 0.072	2.753IlePro: 2.753 ± 0.074	1.471IleGln: 1.471 ± 0.05	2.816IleArg: 2.816 ± 0.076	5.612IleSer: 5.612 ± 0.113	4.55IleThr: 4.55 ± 0.081	4.432IleVal: 4.432 ± 0.085	0.505IleTrp: 0.505 ± 0.032	2.603IleTyr: 2.603 ± 0.063	0.001IleXaa: 0.001 ± 0.001
Lys	5.829LysAla: 5.829 ± 0.095	0.935LysCys: 0.935 ± 0.041	3.68LysAsp: 3.68 ± 0.084	4.342LysGlu: 4.342 ± 0.082	2.331LysPhe: 2.331 ± 0.067	3.964LysGly: 3.964 ± 0.075	1.072LysHis: 1.072 ± 0.041	5.066LysIle: 5.066 ± 0.078	5.979LysLys: 5.979 ± 0.118	6.506LysLeu: 6.506 ± 0.094	2.121LysMet: 2.121 ± 0.049	3.858LysAsn: 3.858 ± 0.09	2.358LysPro: 2.358 ± 0.076	2.176LysGln: 2.176 ± 0.079	3.276LysArg: 3.276 ± 0.075	3.853LysSer: 3.853 ± 0.075	4.212LysThr: 4.212 ± 0.085	4.022LysVal: 4.022 ± 0.083	0.556LysTrp: 0.556 ± 0.027	3.099LysTyr: 3.099 ± 0.083	0.001LysXaa: 0.001 ± 0.002
Leu	7.313LeuAla: 7.313 ± 0.117	1.59LeuCys: 1.59 ± 0.058	5.143LeuAsp: 5.143 ± 0.097	5.412LeuGlu: 5.412 ± 0.102	3.304LeuPhe: 3.304 ± 0.076	5.556LeuGly: 5.556 ± 0.097	1.353LeuHis: 1.353 ± 0.048	5.673LeuIle: 5.673 ± 0.096	5.722LeuLys: 5.722 ± 0.087	6.911LeuLeu: 6.911 ± 0.14	2.377LeuMet: 2.377 ± 0.065	3.402LeuAsn: 3.402 ± 0.084	3.43LeuPro: 3.43 ± 0.078	1.815LeuGln: 1.815 ± 0.055	3.863LeuArg: 3.863 ± 0.103	6.029LeuSer: 6.029 ± 0.104	5.251LeuThr: 5.251 ± 0.097	4.625LeuVal: 4.625 ± 0.087	0.635LeuTrp: 0.635 ± 0.033	3.042LeuTyr: 3.042 ± 0.07	0.004LeuXaa: 0.004 ± 0.002
Met	2.543MetAla: 2.543 ± 0.056	0.455MetCys: 0.455 ± 0.026	1.474MetAsp: 1.474 ± 0.044	1.58MetGlu: 1.58 ± 0.049	0.981MetPhe: 0.981 ± 0.044	1.92MetGly: 1.92 ± 0.057	0.395MetHis: 0.395 ± 0.025	2.058MetIle: 2.058 ± 0.055	2.324MetLys: 2.324 ± 0.063	2.994MetLeu: 2.994 ± 0.07	0.828MetMet: 0.828 ± 0.04	1.492MetAsn: 1.492 ± 0.045	1.147MetPro: 1.147 ± 0.046	0.986MetGln: 0.986 ± 0.033	1.408MetArg: 1.408 ± 0.049	1.894MetSer: 1.894 ± 0.052	1.724MetThr: 1.724 ± 0.06	1.547MetVal: 1.547 ± 0.042	0.214MetTrp: 0.214 ± 0.019	1.012MetTyr: 1.012 ± 0.037	0.001MetXaa: 0.001 ± 0.001
Asn	3.896AsnAla: 3.896 ± 0.097	0.803AsnCys: 0.803 ± 0.036	2.565AsnAsp: 2.565 ± 0.07	2.93AsnGlu: 2.93 ± 0.068	1.764AsnPhe: 1.764 ± 0.052	3.98AsnGly: 3.98 ± 0.093	0.543AsnHis: 0.543 ± 0.034	3.56AsnIle: 3.56 ± 0.086	2.728AsnLys: 2.728 ± 0.075	2.877AsnLeu: 2.877 ± 0.07	1.334AsnMet: 1.334 ± 0.04	1.988AsnAsn: 1.988 ± 0.072	1.616AsnPro: 1.616 ± 0.051	0.927AsnGln: 0.927 ± 0.04	1.583AsnArg: 1.583 ± 0.05	3.104AsnSer: 3.104 ± 0.069	2.5AsnThr: 2.5 ± 0.078	3.022AsnVal: 3.022 ± 0.079	0.417AsnTrp: 0.417 ± 0.029	1.83AsnTyr: 1.83 ± 0.063	0.003AsnXaa: 0.003 ± 0.002
Pro	3.332ProAla: 3.332 ± 0.101	0.556ProCys: 0.556 ± 0.028	2.451ProAsp: 2.451 ± 0.063	3.205ProGlu: 3.205 ± 0.082	1.375ProPhe: 1.375 ± 0.049	1.425ProGly: 1.425 ± 0.05	0.639ProHis: 0.639 ± 0.036	1.999ProIle: 1.999 ± 0.053	2.097ProLys: 2.097 ± 0.063	2.567ProLeu: 2.567 ± 0.056	0.859ProMet: 0.859 ± 0.039	1.463ProAsn: 1.463 ± 0.051	1.006ProPro: 1.006 ± 0.046	1.408ProGln: 1.408 ± 0.055	1.172ProArg: 1.172 ± 0.043	1.793ProSer: 1.793 ± 0.048	1.798ProThr: 1.798 ± 0.048	2.377ProVal: 2.377 ± 0.068	0.25ProTrp: 0.25 ± 0.021	1.346ProTyr: 1.346 ± 0.049	0.004ProXaa: 0.004 ± 0.002
Gln	2.289GlnAla: 2.289 ± 0.068	0.291GlnCys: 0.291 ± 0.022	1.199GlnAsp: 1.199 ± 0.048	1.626GlnGlu: 1.626 ± 0.049	1.053GlnPhe: 1.053 ± 0.032	1.704GlnGly: 1.704 ± 0.047	0.468GlnHis: 0.468 ± 0.031	2.152GlnIle: 2.152 ± 0.061	1.953GlnLys: 1.953 ± 0.058	2.583GlnLeu: 2.583 ± 0.066	0.873GlnMet: 0.873 ± 0.04	1.429GlnAsn: 1.429 ± 0.053	1.017GlnPro: 1.017 ± 0.04	1.173GlnGln: 1.173 ± 0.051	1.442GlnArg: 1.442 ± 0.051	1.6GlnSer: 1.6 ± 0.047	1.762GlnThr: 1.762 ± 0.053	1.47GlnVal: 1.47 ± 0.046	0.214GlnTrp: 0.214 ± 0.02	1.237GlnTyr: 1.237 ± 0.048	0.003GlnXaa: 0.003 ± 0.002
Arg	3.573ArgAla: 3.573 ± 0.102	0.72ArgCys: 0.72 ± 0.032	2.203ArgAsp: 2.203 ± 0.062	3.453ArgGlu: 3.453 ± 0.082	1.948ArgPhe: 1.948 ± 0.049	2.786ArgGly: 2.786 ± 0.075	0.725ArgHis: 0.725 ± 0.039	3.073ArgIle: 3.073 ± 0.072	2.892ArgLys: 2.892 ± 0.062	4.129ArgLeu: 4.129 ± 0.087	1.218ArgMet: 1.218 ± 0.048	1.72ArgAsn: 1.72 ± 0.05	1.291ArgPro: 1.291 ± 0.049	1.255ArgGln: 1.255 ± 0.049	2.798ArgArg: 2.798 ± 0.095	2.753ArgSer: 2.753 ± 0.065	2.176ArgThr: 2.176 ± 0.054	2.747ArgVal: 2.747 ± 0.064	0.38ArgTrp: 0.38 ± 0.024	1.841ArgTyr: 1.841 ± 0.055	0.004ArgXaa: 0.004 ± 0.003
Ser	6.801SerAla: 6.801 ± 0.117	0.989SerCys: 0.989 ± 0.042	4.483SerAsp: 4.483 ± 0.085	4.737SerGlu: 4.737 ± 0.089	2.688SerPhe: 2.688 ± 0.067	5.701SerGly: 5.701 ± 0.118	0.951SerHis: 0.951 ± 0.042	3.733SerIle: 3.733 ± 0.073	3.683SerLys: 3.683 ± 0.085	4.745SerLeu: 4.745 ± 0.085	1.653SerMet: 1.653 ± 0.048	2.282SerAsn: 2.282 ± 0.071	1.929SerPro: 1.929 ± 0.047	1.931SerGln: 1.931 ± 0.061	2.666SerArg: 2.666 ± 0.065	4.44SerSer: 4.44 ± 0.142	3.039SerThr: 3.039 ± 0.073	4.634SerVal: 4.634 ± 0.097	0.525SerTrp: 0.525 ± 0.029	2.497SerTyr: 2.497 ± 0.068	0.003SerXaa: 0.003 ± 0.002
Thr	6.032ThrAla: 6.032 ± 0.113	0.739ThrCys: 0.739 ± 0.039	3.825ThrAsp: 3.825 ± 0.083	3.905ThrGlu: 3.905 ± 0.075	2.165ThrPhe: 2.165 ± 0.061	4.125ThrGly: 4.125 ± 0.091	0.92ThrHis: 0.92 ± 0.03	3.731ThrIle: 3.731 ± 0.094	3.038ThrLys: 3.038 ± 0.085	5.124ThrLeu: 5.124 ± 0.098	1.261ThrMet: 1.261 ± 0.041	1.972ThrAsn: 1.972 ± 0.067	2.333ThrPro: 2.333 ± 0.056	1.604ThrGln: 1.604 ± 0.048	1.897ThrArg: 1.897 ± 0.05	3.161ThrSer: 3.161 ± 0.073	2.767ThrThr: 2.767 ± 0.08	4.483ThrVal: 4.483 ± 0.096	0.41ThrTrp: 0.41 ± 0.028	2.094ThrTyr: 2.094 ± 0.066	0.004ThrXaa: 0.004 ± 0.002
Val	4.424ValAla: 4.424 ± 0.084	1.477ValCys: 1.477 ± 0.049	3.497ValAsp: 3.497 ± 0.084	3.74ValGlu: 3.74 ± 0.085	2.877ValPhe: 2.877 ± 0.064	3.948ValGly: 3.948 ± 0.085	1.059ValHis: 1.059 ± 0.039	5.207ValIle: 5.207 ± 0.088	4.882ValLys: 4.882 ± 0.091	5.675ValLeu: 5.675 ± 0.095	1.973ValMet: 1.973 ± 0.054	2.973ValAsn: 2.973 ± 0.076	2.406ValPro: 2.406 ± 0.064	1.735ValGln: 1.735 ± 0.052	2.889ValArg: 2.889 ± 0.06	4.704ValSer: 4.704 ± 0.088	4.249ValThr: 4.249 ± 0.107	4.104ValVal: 4.104 ± 0.087	0.567ValTrp: 0.567 ± 0.033	2.839ValTyr: 2.839 ± 0.071	0.003ValXaa: 0.003 ± 0.002
Trp	0.502TrpAla: 0.502 ± 0.03	0.18TrpCys: 0.18 ± 0.017	0.503TrpAsp: 0.503 ± 0.028	0.491TrpGlu: 0.491 ± 0.03	0.376TrpPhe: 0.376 ± 0.026	0.626TrpGly: 0.626 ± 0.035	0.183TrpHis: 0.183 ± 0.017	0.467TrpIle: 0.467 ± 0.026	0.525TrpLys: 0.525 ± 0.031	0.75TrpLeu: 0.75 ± 0.032	0.218TrpMet: 0.218 ± 0.02	0.465TrpAsn: 0.465 ± 0.031	0.104TrpPro: 0.104 ± 0.014	0.355TrpGln: 0.355 ± 0.026	0.351TrpArg: 0.351 ± 0.024	0.516TrpSer: 0.516 ± 0.035	0.443TrpThr: 0.443 ± 0.029	0.458TrpVal: 0.458 ± 0.028	0.095TrpTrp: 0.095 ± 0.011	0.418TrpTyr: 0.418 ± 0.028	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.076TyrAla: 3.076 ± 0.069	0.649TyrCys: 0.649 ± 0.028	2.89TyrAsp: 2.89 ± 0.072	2.748TyrGlu: 2.748 ± 0.064	1.858TyrPhe: 1.858 ± 0.061	3.074TyrGly: 3.074 ± 0.077	0.623TyrHis: 0.623 ± 0.03	2.895TyrIle: 2.895 ± 0.065	2.599TyrLys: 2.599 ± 0.065	3.061TyrLeu: 3.061 ± 0.08	1.053TyrMet: 1.053 ± 0.041	1.956TyrAsn: 1.956 ± 0.06	1.392TyrPro: 1.392 ± 0.051	0.986TyrGln: 0.986 ± 0.039	1.951TyrArg: 1.951 ± 0.054	2.859TyrSer: 2.859 ± 0.069	2.609TyrThr: 2.609 ± 0.084	2.482TyrVal: 2.482 ± 0.069	0.358TyrTrp: 0.358 ± 0.026	1.938TyrTyr: 1.938 ± 0.074	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.02XaaAla: 0.02 ± 0.005	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.004XaaGlu: 0.004 ± 0.002	0.0XaaPhe: 0.0 ± 0.0	0.006XaaGly: 0.006 ± 0.003	0.001XaaHis: 0.001 ± 0.001	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.004XaaLeu: 0.004 ± 0.002	0.003XaaMet: 0.003 ± 0.002	0.003XaaAsn: 0.003 ± 0.002	0.006XaaPro: 0.006 ± 0.003	0.003XaaGln: 0.003 ± 0.002	0.006XaaArg: 0.006 ± 0.003	0.003XaaSer: 0.003 ± 0.002	0.0XaaThr: 0.0 ± 0.0	0.003XaaVal: 0.003 ± 0.002	0.003XaaTrp: 0.003 ± 0.002	0.004XaaTyr: 0.004 ± 0.002	0.095XaaXaa: 0.095 ± 0.016

Statistics based on 2155 proteins (683710 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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