Marine Group I thaumarchaeote SCGC AAA799-P11
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1432 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A087S1V8|A0A087S1V8_9ARCH Nitrous-oxide reductase protein OS=Marine Group I thaumarchaeote SCGC AAA799-P11 OX=1502295 GN=nosZ PE=4 SV=1
MM1 pKa = 7.35 IKK3 pKa = 10.23 KK4 pKa = 9.98 IIASSIVGIAIIVSLAVAFSTEE26 pKa = 4.32 TEE28 pKa = 4.24 PQPEE32 pKa = 4.44 PAPEE36 pKa = 4.12 SNPTYY41 pKa = 11.1 SMQGVLQSDD50 pKa = 4.15 VMTGGDD56 pKa = 3.76 VIMPTKK62 pKa = 10.47 VSRR65 pKa = 11.84 PGCEE69 pKa = 3.75 EE70 pKa = 3.52 TDD72 pKa = 2.94 RR73 pKa = 11.84 CYY75 pKa = 10.45 IPSVITISAGDD86 pKa = 3.59 SVTWVNEE93 pKa = 3.41 DD94 pKa = 3.67 SAFHH98 pKa = 6.22 SVTSGFYY105 pKa = 10.48 DD106 pKa = 3.75 EE107 pKa = 5.24 PQDD110 pKa = 4.78 LFDD113 pKa = 5.1 SGHH116 pKa = 6.38 MDD118 pKa = 3.44 PFDD121 pKa = 4.82 SYY123 pKa = 11.79 TLTFDD128 pKa = 3.36 EE129 pKa = 5.7 KK130 pKa = 10.63 GTFDD134 pKa = 4.06 YY135 pKa = 11.13 FCTLHH140 pKa = 6.66 PWMEE144 pKa = 4.16 GQVIVQQ150 pKa = 3.79
Molecular weight: 16.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.774
IPC2_protein 3.986
IPC_protein 3.948
Toseland 3.745
ProMoST 4.101
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.846
Rodwell 3.77
Grimsley 3.656
Solomon 3.91
Lehninger 3.872
Nozaki 4.037
DTASelect 4.24
Thurlkill 3.795
EMBOSS 3.859
Sillero 4.062
Patrickios 0.998
IPC_peptide 3.91
IPC2_peptide 4.037
IPC2.peptide.svr19 3.928
Protein with the highest isoelectric point:
>tr|A0A087S226|A0A087S226_9ARCH Sigma 54 modulation / protein OS=Marine Group I thaumarchaeote SCGC AAA799-P11 OX=1502295 GN=AAA799P11_00525 PE=4 SV=1
MM1 pKa = 7.47 AQTKK5 pKa = 8.86 AQRR8 pKa = 11.84 SAAAKK13 pKa = 9.49 KK14 pKa = 9.83 AARR17 pKa = 11.84 TRR19 pKa = 11.84 KK20 pKa = 9.81 RR21 pKa = 11.84 NAKK24 pKa = 9.85 LKK26 pKa = 11.0 AEE28 pKa = 4.38 AAAKK32 pKa = 10.17 AAVKK36 pKa = 10.37 RR37 pKa = 11.84 KK38 pKa = 8.37 RR39 pKa = 11.84 TAAAKK44 pKa = 9.75 KK45 pKa = 9.75 RR46 pKa = 11.84 AVASAPKK53 pKa = 9.57 KK54 pKa = 9.42 RR55 pKa = 11.84 SVSKK59 pKa = 10.75 RR60 pKa = 11.84 STSKK64 pKa = 10.69 RR65 pKa = 11.84 KK66 pKa = 7.61 TVSKK70 pKa = 10.49 KK71 pKa = 10.72 APTKK75 pKa = 10.42 RR76 pKa = 11.84 KK77 pKa = 9.17 ASKK80 pKa = 10.22 KK81 pKa = 9.75 AVPKK85 pKa = 10.47 RR86 pKa = 11.84 KK87 pKa = 7.66 TAVKK91 pKa = 10.46 SKK93 pKa = 10.31 GNKK96 pKa = 8.57 RR97 pKa = 3.39
Molecular weight: 10.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.46
IPC2_protein 11.082
IPC_protein 12.588
Toseland 12.778
ProMoST 13.247
Dawson 12.778
Bjellqvist 12.749
Wikipedia 13.232
Rodwell 12.778
Grimsley 12.808
Solomon 13.247
Lehninger 13.159
Nozaki 12.764
DTASelect 12.749
Thurlkill 12.764
EMBOSS 13.261
Sillero 12.764
Patrickios 12.486
IPC_peptide 13.261
IPC2_peptide 12.237
IPC2.peptide.svr19 8.998
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1432
0
1432
319401
19
1477
223.0
25.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.802 ± 0.073
1.077 ± 0.03
5.899 ± 0.081
7.377 ± 0.076
4.43 ± 0.053
6.208 ± 0.073
1.802 ± 0.034
8.638 ± 0.075
8.943 ± 0.101
8.521 ± 0.072
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.548 ± 0.037
4.981 ± 0.06
3.729 ± 0.046
3.282 ± 0.043
3.53 ± 0.059
7.16 ± 0.056
5.508 ± 0.066
6.523 ± 0.058
0.908 ± 0.025
3.092 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here