Streptococcus sp. DD13
Average proteome isoelectric point is 6.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1649 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A139NPE3|A0A139NPE3_9STRE Thiamin pyrophosphokinase OS=Streptococcus sp. DD13 OX=1777881 GN=STRDD13_01381 PE=4 SV=1
MM1 pKa = 6.17 MAKK4 pKa = 10.33 YY5 pKa = 10.33 GFLDD9 pKa = 3.25 ILVDD13 pKa = 4.22 VMDD16 pKa = 4.02 QNFSYY21 pKa = 10.39 DD22 pKa = 4.48 YY23 pKa = 10.58 EE24 pKa = 4.5 INWDD28 pKa = 3.8 KK29 pKa = 11.2 KK30 pKa = 7.99 NHH32 pKa = 5.22 AVEE35 pKa = 4.32 VTFVLEE41 pKa = 4.24 AQNQAGVEE49 pKa = 4.25 TVDD52 pKa = 5.07 DD53 pKa = 4.12 SGTASGEE60 pKa = 4.14 DD61 pKa = 3.26 IVFEE65 pKa = 5.1 DD66 pKa = 4.18 YY67 pKa = 11.62 VLFYY71 pKa = 11.26 NPAKK75 pKa = 10.12 TRR77 pKa = 11.84 FDD79 pKa = 3.43 AADD82 pKa = 3.58 YY83 pKa = 10.72 LVAIPYY89 pKa = 8.64 EE90 pKa = 4.19 PKK92 pKa = 10.36 KK93 pKa = 10.93 GLSQEE98 pKa = 3.88 FLAYY102 pKa = 9.77 FAEE105 pKa = 4.37 TLNEE109 pKa = 3.99 VANDD113 pKa = 4.08 GLSDD117 pKa = 4.69 LMDD120 pKa = 4.54 FLADD124 pKa = 4.08 DD125 pKa = 4.16 GAEE128 pKa = 4.02 EE129 pKa = 5.26 FGLVWDD135 pKa = 4.29 QEE137 pKa = 4.2 AFKK140 pKa = 11.06 AGLANLEE147 pKa = 4.03 EE148 pKa = 4.68 TTFYY152 pKa = 10.61 PYY154 pKa = 10.36 PRR156 pKa = 11.84 YY157 pKa = 10.24
Molecular weight: 17.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.707
IPC2_protein 3.77
IPC_protein 3.757
Toseland 3.554
ProMoST 3.91
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.668
Rodwell 3.579
Grimsley 3.465
Solomon 3.732
Lehninger 3.681
Nozaki 3.859
DTASelect 4.062
Thurlkill 3.605
EMBOSS 3.681
Sillero 3.872
Patrickios 1.036
IPC_peptide 3.732
IPC2_peptide 3.859
IPC2.peptide.svr19 3.787
Protein with the highest isoelectric point:
>tr|A0A139NNZ5|A0A139NNZ5_9STRE Dihydrofolate reductase OS=Streptococcus sp. DD13 OX=1777881 GN=STRDD13_01327 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 9.26 IRR11 pKa = 11.84 RR12 pKa = 11.84 ARR14 pKa = 11.84 KK15 pKa = 8.35 HH16 pKa = 4.78 GFRR19 pKa = 11.84 NRR21 pKa = 11.84 MSTKK25 pKa = 9.22 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.81 GRR39 pKa = 11.84 KK40 pKa = 8.95 VLTAAA45 pKa = 4.49
Molecular weight: 5.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.525
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.056
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.735
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.554
Sillero 13.042
Patrickios 12.457
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.203
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1649
0
1649
483867
37
1543
293.4
32.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.72 ± 0.076
0.493 ± 0.015
5.55 ± 0.042
7.133 ± 0.061
4.478 ± 0.052
6.818 ± 0.058
2.022 ± 0.029
6.841 ± 0.059
5.943 ± 0.056
10.298 ± 0.089
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.425 ± 0.03
3.92 ± 0.044
3.468 ± 0.033
4.611 ± 0.052
4.573 ± 0.053
6.357 ± 0.091
5.667 ± 0.053
7.106 ± 0.053
0.889 ± 0.022
3.69 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here