Roseomonas rosea
Average proteome isoelectric point is 6.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5011 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1M6MB58|A0A1M6MB58_9PROT Dolichol-phosphate mannosyltransferase OS=Roseomonas rosea OX=198092 GN=SAMN02745194_03415 PE=4 SV=1
MM1 pKa = 7.9 PATTSPSASGTPAIDD16 pKa = 3.55 GLLAGTAWAGHH27 pKa = 5.6 SLTYY31 pKa = 10.53 SFPGSAADD39 pKa = 4.01 LSDD42 pKa = 3.59 YY43 pKa = 10.68 SAPNAVDD50 pKa = 3.61 PSYY53 pKa = 11.45 VAALSSGQQDD63 pKa = 3.81 TVRR66 pKa = 11.84 GMLVAFGAVANITFSEE82 pKa = 4.93 AITPSGGDD90 pKa = 3.33 LRR92 pKa = 11.84 LYY94 pKa = 9.86 WYY96 pKa = 7.65 EE97 pKa = 4.35 APDD100 pKa = 3.53 NYY102 pKa = 9.82 TARR105 pKa = 11.84 TVEE108 pKa = 4.45 FPSANPEE115 pKa = 4.12 AGDD118 pKa = 3.85 VQLGAYY124 pKa = 7.51 VVPGFVDD131 pKa = 3.0 QWTLGSYY138 pKa = 10.46 SYY140 pKa = 9.47 FTILHH145 pKa = 5.95 EE146 pKa = 4.91 LGHH149 pKa = 6.52 ALGLKK154 pKa = 9.8 HH155 pKa = 6.56 PHH157 pKa = 6.93 DD158 pKa = 4.68 EE159 pKa = 4.6 VNGFPPIPAAQDD171 pKa = 3.57 SIEE174 pKa = 3.96 YY175 pKa = 10.47 SIMSYY180 pKa = 10.43 RR181 pKa = 11.84 SYY183 pKa = 11.2 AGQDD187 pKa = 3.08 FGGYY191 pKa = 6.37 TVQSGSYY198 pKa = 9.79 PSGPMLNDD206 pKa = 3.21 IAALQYY212 pKa = 11.02 LYY214 pKa = 10.89 GANWTTNSGDD224 pKa = 3.05 TTYY227 pKa = 11.21 RR228 pKa = 11.84 FDD230 pKa = 3.85 PEE232 pKa = 3.98 QAVVFQTIWDD242 pKa = 3.87 GGGEE246 pKa = 4.06 DD247 pKa = 4.3 TYY249 pKa = 11.89 DD250 pKa = 3.44 LSAYY254 pKa = 8.44 GTDD257 pKa = 4.13 LVISLDD263 pKa = 3.42 PGGWSRR269 pKa = 11.84 FGGQYY274 pKa = 11.07 AVLDD278 pKa = 4.08 TQDD281 pKa = 2.86 GRR283 pKa = 11.84 EE284 pKa = 4.31 AIGNVANAYY293 pKa = 9.98 LYY295 pKa = 10.76 QGDD298 pKa = 4.22 LRR300 pKa = 11.84 SLIEE304 pKa = 3.87 NAIGGSGNDD313 pKa = 4.04 EE314 pKa = 3.75 ITGNAGANALTGGAGDD330 pKa = 3.59 DD331 pKa = 3.83 TLRR334 pKa = 11.84 GGLGDD339 pKa = 3.69 DD340 pKa = 3.82 VLRR343 pKa = 11.84 GGSGIDD349 pKa = 3.43 LMVGGEE355 pKa = 4.24 GSDD358 pKa = 3.64 TYY360 pKa = 11.21 HH361 pKa = 6.72 VDD363 pKa = 3.28 SVGDD367 pKa = 3.73 RR368 pKa = 11.84 VRR370 pKa = 11.84 EE371 pKa = 3.9 AAGEE375 pKa = 4.27 GGDD378 pKa = 4.06 VVWTGVDD385 pKa = 3.0 WTLEE389 pKa = 3.79 AGQAVEE395 pKa = 4.71 VIRR398 pKa = 11.84 ANAGEE403 pKa = 4.44 AGLTLTGNEE412 pKa = 3.96 LGNWLVSGAGADD424 pKa = 3.87 LLIGGLGNDD433 pKa = 3.26 SLYY436 pKa = 11.42 VNSAEE441 pKa = 4.07 DD442 pKa = 3.23 RR443 pKa = 11.84 VVEE446 pKa = 4.34 LVGQGSDD453 pKa = 3.31 TVFASASYY461 pKa = 9.46 TLAAGQEE468 pKa = 4.14 IEE470 pKa = 4.37 ILRR473 pKa = 11.84 ANAGATGLVLAGNDD487 pKa = 2.85 IANRR491 pKa = 11.84 IVGGDD496 pKa = 3.53 GADD499 pKa = 3.56 TLSGALGQDD508 pKa = 2.88 TLMGGEE514 pKa = 4.11 GADD517 pKa = 3.23 VFRR520 pKa = 11.84 FSSLAEE526 pKa = 4.32 SPASMLRR533 pKa = 11.84 DD534 pKa = 3.78 YY535 pKa = 11.18 ISDD538 pKa = 3.95 FVSGVDD544 pKa = 5.5 LINLSAIQAVAGAAVDD560 pKa = 3.51 AAFTFLDD567 pKa = 3.71 KK568 pKa = 11.42 DD569 pKa = 3.91 SFTKK573 pKa = 10.5 QAGQLHH579 pKa = 5.61 QIKK582 pKa = 10.24 AGSNTVVEE590 pKa = 4.48 GDD592 pKa = 3.62 VNGDD596 pKa = 2.97 GRR598 pKa = 11.84 ADD600 pKa = 3.62 LQILLKK606 pKa = 10.89 GHH608 pKa = 7.44 LDD610 pKa = 3.68 LEE612 pKa = 5.34 AGDD615 pKa = 4.39 FLLL618 pKa = 5.79
Molecular weight: 63.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.689
IPC2_protein 3.808
IPC_protein 3.846
Toseland 3.617
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.986
Wikipedia 3.783
Rodwell 3.668
Grimsley 3.516
Solomon 3.834
Lehninger 3.795
Nozaki 3.948
DTASelect 4.215
Thurlkill 3.668
EMBOSS 3.783
Sillero 3.973
Patrickios 1.456
IPC_peptide 3.834
IPC2_peptide 3.948
IPC2.peptide.svr19 3.85
Protein with the highest isoelectric point:
>tr|A0A1M6Q9A7|A0A1M6Q9A7_9PROT General L-amino acid transport system substrate-binding protein OS=Roseomonas rosea OX=198092 GN=SAMN02745194_04339 PE=3 SV=1
MM1 pKa = 7.34 LVGRR5 pKa = 11.84 IAKK8 pKa = 9.96 ALIGAAIRR16 pKa = 11.84 RR17 pKa = 11.84 QQNRR21 pKa = 11.84 ALGSIGLGGWKK32 pKa = 9.78 LHH34 pKa = 6.6 LFRR37 pKa = 11.84 AVQAAMRR44 pKa = 11.84 QGRR47 pKa = 11.84 RR48 pKa = 11.84 GPGQPMPRR56 pKa = 11.84 PPRR59 pKa = 11.84 RR60 pKa = 11.84 DD61 pKa = 2.91 IGG63 pKa = 3.55
Molecular weight: 6.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.416
IPC2_protein 10.891
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.13
Rodwell 12.237
Grimsley 12.691
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 11.974
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.128
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5011
0
5011
1607065
39
2482
320.7
34.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.729 ± 0.055
0.768 ± 0.009
4.94 ± 0.024
5.845 ± 0.033
3.207 ± 0.019
9.496 ± 0.036
1.999 ± 0.017
4.026 ± 0.023
1.907 ± 0.023
10.926 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.439 ± 0.016
2.047 ± 0.016
6.412 ± 0.027
2.815 ± 0.017
8.556 ± 0.036
4.592 ± 0.021
4.896 ± 0.021
7.243 ± 0.026
1.42 ± 0.015
1.738 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here