Iris yellow spot virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Tospoviridae; Orthotospovirus; Iris yellow spot orthotospovirus

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|C0KQW8|C0KQW8_9VIRU Replicase OS=Iris yellow spot virus OX=60456 PE=4 SV=1
MM1 pKa = 7.27NLHH4 pKa = 6.51YY5 pKa = 10.95LLLYY9 pKa = 10.52CFGVYY14 pKa = 10.61LMFTITDD21 pKa = 3.57VLLMNNAEE29 pKa = 4.02DD30 pKa = 4.13HH31 pKa = 6.86EE32 pKa = 4.85DD33 pKa = 2.9IKK35 pKa = 11.1MEE37 pKa = 4.6LKK39 pKa = 10.43RR40 pKa = 11.84LKK42 pKa = 10.42DD43 pKa = 3.27RR44 pKa = 11.84YY45 pKa = 10.87KK46 pKa = 11.03NDD48 pKa = 3.56DD49 pKa = 3.93PEE51 pKa = 5.33DD52 pKa = 4.49LIEE55 pKa = 4.92NKK57 pKa = 10.16VIDD60 pKa = 4.4PGYY63 pKa = 10.13SFPSLDD69 pKa = 3.3PKK71 pKa = 10.53IPEE74 pKa = 4.67RR75 pKa = 11.84IPRR78 pKa = 11.84EE79 pKa = 3.71ATTNVPSTTNVPSTTDD95 pKa = 3.21RR96 pKa = 11.84QVVEE100 pKa = 4.6TLSCDD105 pKa = 3.9DD106 pKa = 4.15FDD108 pKa = 5.4KK109 pKa = 11.17SKK111 pKa = 11.07CIIKK115 pKa = 8.88GTSDD119 pKa = 3.71FNAHH123 pKa = 5.15YY124 pKa = 9.49QVKK127 pKa = 10.5GSDD130 pKa = 3.54SVIACITDD138 pKa = 3.33SEE140 pKa = 4.98KK141 pKa = 10.82IFDD144 pKa = 3.96ACLFDD149 pKa = 5.57SDD151 pKa = 3.42IKK153 pKa = 10.95RR154 pKa = 11.84RR155 pKa = 11.84TFLKK159 pKa = 10.43VPVVPVLKK167 pKa = 10.8LEE169 pKa = 4.2NKK171 pKa = 9.7RR172 pKa = 11.84VLEE175 pKa = 4.18VGSKK179 pKa = 10.11FFFVDD184 pKa = 3.69SSNDD188 pKa = 4.01PILSDD193 pKa = 4.32PKK195 pKa = 10.92SGMANPTVSRR205 pKa = 11.84LSVRR209 pKa = 11.84LSGDD213 pKa = 3.57CIINQVSMSSPYY225 pKa = 9.23MIKK228 pKa = 10.05IKK230 pKa = 9.66STEE233 pKa = 4.17TIGFAVKK240 pKa = 10.41NIKK243 pKa = 10.03NPQAINFQTASGDD256 pKa = 3.08KK257 pKa = 10.33SVYY260 pKa = 10.38FKK262 pKa = 10.52IDD264 pKa = 3.52EE265 pKa = 4.43LDD267 pKa = 3.69GNHH270 pKa = 6.31FFLCGDD276 pKa = 3.18KK277 pKa = 11.08SSFIKK282 pKa = 10.69KK283 pKa = 9.47VDD285 pKa = 3.46VPVRR289 pKa = 11.84NCVSKK294 pKa = 11.27YY295 pKa = 10.19ADD297 pKa = 3.58EE298 pKa = 4.34PKK300 pKa = 10.64KK301 pKa = 10.8IFFCTNFSYY310 pKa = 10.04FKK312 pKa = 10.28WFFTFLVITFPITWILWKK330 pKa = 10.46AKK332 pKa = 9.43NAFSIWYY339 pKa = 8.8DD340 pKa = 2.83IMGILTYY347 pKa = 10.28PILISINYY355 pKa = 8.04LWLYY359 pKa = 10.63FPFKK363 pKa = 10.63CKK365 pKa = 10.49SCGNLSFLSHH375 pKa = 6.22EE376 pKa = 4.38CSRR379 pKa = 11.84LCVCNQSKK387 pKa = 10.24PSRR390 pKa = 11.84EE391 pKa = 3.97HH392 pKa = 7.2SKK394 pKa = 10.57EE395 pKa = 3.73CYY397 pKa = 9.77LFHH400 pKa = 7.89KK401 pKa = 8.97DD402 pKa = 3.14TKK404 pKa = 9.93KK405 pKa = 9.73WRR407 pKa = 11.84SLSLIDD413 pKa = 3.64HH414 pKa = 6.51FQFIVNTKK422 pKa = 9.2ISSEE426 pKa = 3.9FLVFLTKK433 pKa = 9.98MLIAGVLMSCIPSSLALSSEE453 pKa = 4.12RR454 pKa = 11.84NVCVEE459 pKa = 3.55KK460 pKa = 10.91CFYY463 pKa = 11.22SKK465 pKa = 10.9DD466 pKa = 3.79LEE468 pKa = 4.38KK469 pKa = 10.66LTTDD473 pKa = 3.27KK474 pKa = 11.33DD475 pKa = 3.85GSIKK479 pKa = 10.72DD480 pKa = 3.99SIDD483 pKa = 3.2TCEE486 pKa = 4.11CSIGEE491 pKa = 4.81LITEE495 pKa = 4.4TVYY498 pKa = 10.87RR499 pKa = 11.84GGAPISRR506 pKa = 11.84AAIKK510 pKa = 10.14NDD512 pKa = 3.37CVSLSSKK519 pKa = 10.4CLNSSNQAEE528 pKa = 4.21NLFACRR534 pKa = 11.84HH535 pKa = 5.08GCNALSTLNKK545 pKa = 9.16IPKK548 pKa = 9.04VMYY551 pKa = 9.64DD552 pKa = 2.87RR553 pKa = 11.84GYY555 pKa = 11.02KK556 pKa = 10.05GVEE559 pKa = 3.7FSGNLTVLKK568 pKa = 10.16IANRR572 pKa = 11.84LRR574 pKa = 11.84KK575 pKa = 10.13GFVDD579 pKa = 3.45NQSEE583 pKa = 4.35VRR585 pKa = 11.84NLEE588 pKa = 4.19SKK590 pKa = 10.24VSKK593 pKa = 9.78EE594 pKa = 3.46LSYY597 pKa = 11.0FKK599 pKa = 10.56SLKK602 pKa = 10.27VDD604 pKa = 3.77DD605 pKa = 4.73VPPEE609 pKa = 3.92NLMPRR614 pKa = 11.84QSLVFSTEE622 pKa = 3.11VDD624 pKa = 3.05GKK626 pKa = 8.53YY627 pKa = 10.23RR628 pKa = 11.84YY629 pKa = 9.01MIEE632 pKa = 3.79MDD634 pKa = 3.16IKK636 pKa = 10.92KK637 pKa = 8.53EE638 pKa = 4.09TGSVFLLNDD647 pKa = 4.14DD648 pKa = 4.19SSHH651 pKa = 7.66IPMEE655 pKa = 3.59FMVYY659 pKa = 8.85VKK661 pKa = 10.53SVGVEE666 pKa = 3.2YY667 pKa = 10.4DD668 pKa = 2.72IRR670 pKa = 11.84YY671 pKa = 9.5KK672 pKa = 11.09YY673 pKa = 9.68STAKK677 pKa = 10.37VDD679 pKa = 3.74TTVTDD684 pKa = 3.76YY685 pKa = 11.68LSTCTGDD692 pKa = 4.76CKK694 pKa = 10.83DD695 pKa = 3.8CRR697 pKa = 11.84KK698 pKa = 10.47SKK700 pKa = 9.71TPSGHH705 pKa = 6.71NDD707 pKa = 2.85FCIQPTSWWGCEE719 pKa = 3.82EE720 pKa = 4.53LGCLAINEE728 pKa = 4.33GAICGHH734 pKa = 5.88CTNVFDD740 pKa = 6.71LSTLVNVYY748 pKa = 9.59QVVEE752 pKa = 3.77SHH754 pKa = 7.14IIAEE758 pKa = 4.15ICIKK762 pKa = 10.55SLNGYY767 pKa = 8.27DD768 pKa = 3.85CRR770 pKa = 11.84KK771 pKa = 10.01HH772 pKa = 6.79SDD774 pKa = 3.3RR775 pKa = 11.84APIQTDD781 pKa = 4.26YY782 pKa = 10.88YY783 pKa = 9.83QLDD786 pKa = 3.82MTVDD790 pKa = 3.42LHH792 pKa = 7.59NDD794 pKa = 3.21FMSTDD799 pKa = 3.18KK800 pKa = 11.32LFAVTKK806 pKa = 8.66NQKK809 pKa = 9.5ILTGSISDD817 pKa = 4.19LSDD820 pKa = 3.93FSSGAFGHH828 pKa = 5.83PQITVDD834 pKa = 3.79GTPLAVKK841 pKa = 9.2ATISRR846 pKa = 11.84DD847 pKa = 3.22QFTWSCSAVGSKK859 pKa = 10.0SVNIKK864 pKa = 9.92QCGLYY869 pKa = 9.56SYY871 pKa = 11.67NMIYY875 pKa = 10.99ALTQSKK881 pKa = 10.43DD882 pKa = 3.32FSVIDD887 pKa = 3.61EE888 pKa = 4.73EE889 pKa = 4.48NNKK892 pKa = 10.63LYY894 pKa = 9.93MIKK897 pKa = 10.51DD898 pKa = 3.6SLVGKK903 pKa = 9.61LKK905 pKa = 10.92VVIDD909 pKa = 3.75MPKK912 pKa = 10.71EE913 pKa = 4.01MFKK916 pKa = 9.53KK917 pKa = 9.2TPVKK921 pKa = 10.31PILSEE926 pKa = 4.02TKK928 pKa = 9.89IICSGCTKK936 pKa = 10.28CAMGLEE942 pKa = 4.51CVMEE946 pKa = 4.2YY947 pKa = 10.46TSDD950 pKa = 3.36TTFSSRR956 pKa = 11.84LKK958 pKa = 10.11MDD960 pKa = 3.74GCSFKK965 pKa = 10.77SDD967 pKa = 3.6QIGSYY972 pKa = 9.9IGPNKK977 pKa = 10.2KK978 pKa = 10.07LIKK981 pKa = 9.74AYY983 pKa = 10.36CSEE986 pKa = 4.21SVKK989 pKa = 11.04DD990 pKa = 3.44KK991 pKa = 9.97TVQVIPEE998 pKa = 4.19DD999 pKa = 3.68QEE1001 pKa = 4.0EE1002 pKa = 4.18LSIEE1006 pKa = 4.1LKK1008 pKa = 9.97IDD1010 pKa = 3.29QVNVIDD1016 pKa = 3.72QDD1018 pKa = 4.27TIISYY1023 pKa = 10.5DD1024 pKa = 3.7DD1025 pKa = 3.83KK1026 pKa = 11.36SAHH1029 pKa = 7.2DD1030 pKa = 4.3EE1031 pKa = 4.55NIHH1034 pKa = 6.64HH1035 pKa = 7.66ADD1037 pKa = 3.26TGIATLWDD1045 pKa = 3.87WIKK1048 pKa = 11.44APFNWVASFFGSFFDD1063 pKa = 3.68LVRR1066 pKa = 11.84VILVILAVIVGLYY1079 pKa = 9.16ILGYY1083 pKa = 9.82IYY1085 pKa = 10.83NLSFSYY1091 pKa = 10.86YY1092 pKa = 9.06KK1093 pKa = 10.32EE1094 pKa = 3.55KK1095 pKa = 10.64RR1096 pKa = 11.84KK1097 pKa = 10.45RR1098 pKa = 11.84KK1099 pKa = 8.85MEE1101 pKa = 4.21FDD1103 pKa = 3.53LEE1105 pKa = 4.14EE1106 pKa = 5.89AEE1108 pKa = 4.13EE1109 pKa = 4.06SLIIKK1114 pKa = 8.8SNKK1117 pKa = 8.26YY1118 pKa = 9.23GEE1120 pKa = 4.27PRR1122 pKa = 11.84RR1123 pKa = 11.84RR1124 pKa = 11.84TPPKK1128 pKa = 9.63TFQFPLDD1135 pKa = 3.67LL1136 pKa = 5.62

Molecular weight:
128.86 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q8V6X1|Q8V6X1_9VIRU Envelopment polyprotein OS=Iris yellow spot virus OX=60456 GN=GP PE=3 SV=1
MM1 pKa = 6.61STVRR5 pKa = 11.84VKK7 pKa = 10.67PSEE10 pKa = 3.94IEE12 pKa = 3.97KK13 pKa = 10.48LLSGGDD19 pKa = 3.19VDD21 pKa = 5.66VVIEE25 pKa = 4.06SDD27 pKa = 3.26EE28 pKa = 4.39TEE30 pKa = 4.08GFNFKK35 pKa = 10.89NFVLANEE42 pKa = 4.8GVQMTFNNGYY52 pKa = 8.29TILRR56 pKa = 11.84NRR58 pKa = 11.84AGIYY62 pKa = 8.65KK63 pKa = 9.27TIKK66 pKa = 8.24TGKK69 pKa = 8.6FTFQGKK75 pKa = 8.15TIVIPSANVSPNQDD89 pKa = 2.3DD90 pKa = 3.58WTFRR94 pKa = 11.84RR95 pKa = 11.84LEE97 pKa = 3.93GFIRR101 pKa = 11.84ARR103 pKa = 11.84MLVEE107 pKa = 4.84LIEE110 pKa = 4.45TKK112 pKa = 10.46DD113 pKa = 3.46EE114 pKa = 4.15KK115 pKa = 10.84EE116 pKa = 3.9KK117 pKa = 11.02QKK119 pKa = 10.27MYY121 pKa = 11.02EE122 pKa = 4.31KK123 pKa = 10.3ICGLPLVSAYY133 pKa = 10.15GLKK136 pKa = 10.34PSSKK140 pKa = 9.73FHH142 pKa = 6.3ATTARR147 pKa = 11.84IMLTLGGPLTLMASLDD163 pKa = 3.54IFAAAALPLAYY174 pKa = 9.83FQNVKK179 pKa = 10.5KK180 pKa = 9.87EE181 pKa = 3.81ALGISRR187 pKa = 11.84FSTYY191 pKa = 9.83EE192 pKa = 3.58QLCKK196 pKa = 10.03VARR199 pKa = 11.84VMAAKK204 pKa = 9.95EE205 pKa = 4.04FKK207 pKa = 9.89FTEE210 pKa = 4.36KK211 pKa = 10.36YY212 pKa = 10.58KK213 pKa = 10.91KK214 pKa = 10.44IFDD217 pKa = 3.76EE218 pKa = 4.78TIKK221 pKa = 10.65ILTDD225 pKa = 3.56CTPGTSGAASLIKK238 pKa = 10.52FNEE241 pKa = 3.98QIKK244 pKa = 10.11ILEE247 pKa = 4.29GAFGKK252 pKa = 10.12IVEE255 pKa = 5.34DD256 pKa = 3.22IGEE259 pKa = 4.16SSKK262 pKa = 11.19PKK264 pKa = 9.98TPSKK268 pKa = 10.28KK269 pKa = 10.0DD270 pKa = 3.08RR271 pKa = 11.84YY272 pKa = 9.62NN273 pKa = 3.03

Molecular weight:
30.5 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

6483

273

2873

926.1

105.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.795 ± 0.529

2.453 ± 0.373

6.355 ± 0.393

6.185 ± 0.383

4.936 ± 0.24

4.257 ± 0.356

1.512 ± 0.146

7.605 ± 0.16

9.27 ± 0.206

8.808 ± 0.612

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.178 ± 0.538

6.047 ± 0.6

3.039 ± 0.384

2.391 ± 0.09

3.378 ± 0.095

9.918 ± 0.281

5.954 ± 0.205

6.016 ± 0.393

0.848 ± 0.126

4.057 ± 0.34

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski