Iris yellow spot virus
Average proteome isoelectric point is 6.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C0KQW8|C0KQW8_9VIRU Replicase OS=Iris yellow spot virus OX=60456 PE=4 SV=1
MM1 pKa = 7.27 NLHH4 pKa = 6.51 YY5 pKa = 10.95 LLLYY9 pKa = 10.52 CFGVYY14 pKa = 10.61 LMFTITDD21 pKa = 3.57 VLLMNNAEE29 pKa = 4.02 DD30 pKa = 4.13 HH31 pKa = 6.86 EE32 pKa = 4.85 DD33 pKa = 2.9 IKK35 pKa = 11.1 MEE37 pKa = 4.6 LKK39 pKa = 10.43 RR40 pKa = 11.84 LKK42 pKa = 10.42 DD43 pKa = 3.27 RR44 pKa = 11.84 YY45 pKa = 10.87 KK46 pKa = 11.03 NDD48 pKa = 3.56 DD49 pKa = 3.93 PEE51 pKa = 5.33 DD52 pKa = 4.49 LIEE55 pKa = 4.92 NKK57 pKa = 10.16 VIDD60 pKa = 4.4 PGYY63 pKa = 10.13 SFPSLDD69 pKa = 3.3 PKK71 pKa = 10.53 IPEE74 pKa = 4.67 RR75 pKa = 11.84 IPRR78 pKa = 11.84 EE79 pKa = 3.71 ATTNVPSTTNVPSTTDD95 pKa = 3.21 RR96 pKa = 11.84 QVVEE100 pKa = 4.6 TLSCDD105 pKa = 3.9 DD106 pKa = 4.15 FDD108 pKa = 5.4 KK109 pKa = 11.17 SKK111 pKa = 11.07 CIIKK115 pKa = 8.88 GTSDD119 pKa = 3.71 FNAHH123 pKa = 5.15 YY124 pKa = 9.49 QVKK127 pKa = 10.5 GSDD130 pKa = 3.54 SVIACITDD138 pKa = 3.33 SEE140 pKa = 4.98 KK141 pKa = 10.82 IFDD144 pKa = 3.96 ACLFDD149 pKa = 5.57 SDD151 pKa = 3.42 IKK153 pKa = 10.95 RR154 pKa = 11.84 RR155 pKa = 11.84 TFLKK159 pKa = 10.43 VPVVPVLKK167 pKa = 10.8 LEE169 pKa = 4.2 NKK171 pKa = 9.7 RR172 pKa = 11.84 VLEE175 pKa = 4.18 VGSKK179 pKa = 10.11 FFFVDD184 pKa = 3.69 SSNDD188 pKa = 4.01 PILSDD193 pKa = 4.32 PKK195 pKa = 10.92 SGMANPTVSRR205 pKa = 11.84 LSVRR209 pKa = 11.84 LSGDD213 pKa = 3.57 CIINQVSMSSPYY225 pKa = 9.23 MIKK228 pKa = 10.05 IKK230 pKa = 9.66 STEE233 pKa = 4.17 TIGFAVKK240 pKa = 10.41 NIKK243 pKa = 10.03 NPQAINFQTASGDD256 pKa = 3.08 KK257 pKa = 10.33 SVYY260 pKa = 10.38 FKK262 pKa = 10.52 IDD264 pKa = 3.52 EE265 pKa = 4.43 LDD267 pKa = 3.69 GNHH270 pKa = 6.31 FFLCGDD276 pKa = 3.18 KK277 pKa = 11.08 SSFIKK282 pKa = 10.69 KK283 pKa = 9.47 VDD285 pKa = 3.46 VPVRR289 pKa = 11.84 NCVSKK294 pKa = 11.27 YY295 pKa = 10.19 ADD297 pKa = 3.58 EE298 pKa = 4.34 PKK300 pKa = 10.64 KK301 pKa = 10.8 IFFCTNFSYY310 pKa = 10.04 FKK312 pKa = 10.28 WFFTFLVITFPITWILWKK330 pKa = 10.46 AKK332 pKa = 9.43 NAFSIWYY339 pKa = 8.8 DD340 pKa = 2.83 IMGILTYY347 pKa = 10.28 PILISINYY355 pKa = 8.04 LWLYY359 pKa = 10.63 FPFKK363 pKa = 10.63 CKK365 pKa = 10.49 SCGNLSFLSHH375 pKa = 6.22 EE376 pKa = 4.38 CSRR379 pKa = 11.84 LCVCNQSKK387 pKa = 10.24 PSRR390 pKa = 11.84 EE391 pKa = 3.97 HH392 pKa = 7.2 SKK394 pKa = 10.57 EE395 pKa = 3.73 CYY397 pKa = 9.77 LFHH400 pKa = 7.89 KK401 pKa = 8.97 DD402 pKa = 3.14 TKK404 pKa = 9.93 KK405 pKa = 9.73 WRR407 pKa = 11.84 SLSLIDD413 pKa = 3.64 HH414 pKa = 6.51 FQFIVNTKK422 pKa = 9.2 ISSEE426 pKa = 3.9 FLVFLTKK433 pKa = 9.98 MLIAGVLMSCIPSSLALSSEE453 pKa = 4.12 RR454 pKa = 11.84 NVCVEE459 pKa = 3.55 KK460 pKa = 10.91 CFYY463 pKa = 11.22 SKK465 pKa = 10.9 DD466 pKa = 3.79 LEE468 pKa = 4.38 KK469 pKa = 10.66 LTTDD473 pKa = 3.27 KK474 pKa = 11.33 DD475 pKa = 3.85 GSIKK479 pKa = 10.72 DD480 pKa = 3.99 SIDD483 pKa = 3.2 TCEE486 pKa = 4.11 CSIGEE491 pKa = 4.81 LITEE495 pKa = 4.4 TVYY498 pKa = 10.87 RR499 pKa = 11.84 GGAPISRR506 pKa = 11.84 AAIKK510 pKa = 10.14 NDD512 pKa = 3.37 CVSLSSKK519 pKa = 10.4 CLNSSNQAEE528 pKa = 4.21 NLFACRR534 pKa = 11.84 HH535 pKa = 5.08 GCNALSTLNKK545 pKa = 9.16 IPKK548 pKa = 9.04 VMYY551 pKa = 9.64 DD552 pKa = 2.87 RR553 pKa = 11.84 GYY555 pKa = 11.02 KK556 pKa = 10.05 GVEE559 pKa = 3.7 FSGNLTVLKK568 pKa = 10.16 IANRR572 pKa = 11.84 LRR574 pKa = 11.84 KK575 pKa = 10.13 GFVDD579 pKa = 3.45 NQSEE583 pKa = 4.35 VRR585 pKa = 11.84 NLEE588 pKa = 4.19 SKK590 pKa = 10.24 VSKK593 pKa = 9.78 EE594 pKa = 3.46 LSYY597 pKa = 11.0 FKK599 pKa = 10.56 SLKK602 pKa = 10.27 VDD604 pKa = 3.77 DD605 pKa = 4.73 VPPEE609 pKa = 3.92 NLMPRR614 pKa = 11.84 QSLVFSTEE622 pKa = 3.11 VDD624 pKa = 3.05 GKK626 pKa = 8.53 YY627 pKa = 10.23 RR628 pKa = 11.84 YY629 pKa = 9.01 MIEE632 pKa = 3.79 MDD634 pKa = 3.16 IKK636 pKa = 10.92 KK637 pKa = 8.53 EE638 pKa = 4.09 TGSVFLLNDD647 pKa = 4.14 DD648 pKa = 4.19 SSHH651 pKa = 7.66 IPMEE655 pKa = 3.59 FMVYY659 pKa = 8.85 VKK661 pKa = 10.53 SVGVEE666 pKa = 3.2 YY667 pKa = 10.4 DD668 pKa = 2.72 IRR670 pKa = 11.84 YY671 pKa = 9.5 KK672 pKa = 11.09 YY673 pKa = 9.68 STAKK677 pKa = 10.37 VDD679 pKa = 3.74 TTVTDD684 pKa = 3.76 YY685 pKa = 11.68 LSTCTGDD692 pKa = 4.76 CKK694 pKa = 10.83 DD695 pKa = 3.8 CRR697 pKa = 11.84 KK698 pKa = 10.47 SKK700 pKa = 9.71 TPSGHH705 pKa = 6.71 NDD707 pKa = 2.85 FCIQPTSWWGCEE719 pKa = 3.82 EE720 pKa = 4.53 LGCLAINEE728 pKa = 4.33 GAICGHH734 pKa = 5.88 CTNVFDD740 pKa = 6.71 LSTLVNVYY748 pKa = 9.59 QVVEE752 pKa = 3.77 SHH754 pKa = 7.14 IIAEE758 pKa = 4.15 ICIKK762 pKa = 10.55 SLNGYY767 pKa = 8.27 DD768 pKa = 3.85 CRR770 pKa = 11.84 KK771 pKa = 10.01 HH772 pKa = 6.79 SDD774 pKa = 3.3 RR775 pKa = 11.84 APIQTDD781 pKa = 4.26 YY782 pKa = 10.88 YY783 pKa = 9.83 QLDD786 pKa = 3.82 MTVDD790 pKa = 3.42 LHH792 pKa = 7.59 NDD794 pKa = 3.21 FMSTDD799 pKa = 3.18 KK800 pKa = 11.32 LFAVTKK806 pKa = 8.66 NQKK809 pKa = 9.5 ILTGSISDD817 pKa = 4.19 LSDD820 pKa = 3.93 FSSGAFGHH828 pKa = 5.83 PQITVDD834 pKa = 3.79 GTPLAVKK841 pKa = 9.2 ATISRR846 pKa = 11.84 DD847 pKa = 3.22 QFTWSCSAVGSKK859 pKa = 10.0 SVNIKK864 pKa = 9.92 QCGLYY869 pKa = 9.56 SYY871 pKa = 11.67 NMIYY875 pKa = 10.99 ALTQSKK881 pKa = 10.43 DD882 pKa = 3.32 FSVIDD887 pKa = 3.61 EE888 pKa = 4.73 EE889 pKa = 4.48 NNKK892 pKa = 10.63 LYY894 pKa = 9.93 MIKK897 pKa = 10.51 DD898 pKa = 3.6 SLVGKK903 pKa = 9.61 LKK905 pKa = 10.92 VVIDD909 pKa = 3.75 MPKK912 pKa = 10.71 EE913 pKa = 4.01 MFKK916 pKa = 9.53 KK917 pKa = 9.2 TPVKK921 pKa = 10.31 PILSEE926 pKa = 4.02 TKK928 pKa = 9.89 IICSGCTKK936 pKa = 10.28 CAMGLEE942 pKa = 4.51 CVMEE946 pKa = 4.2 YY947 pKa = 10.46 TSDD950 pKa = 3.36 TTFSSRR956 pKa = 11.84 LKK958 pKa = 10.11 MDD960 pKa = 3.74 GCSFKK965 pKa = 10.77 SDD967 pKa = 3.6 QIGSYY972 pKa = 9.9 IGPNKK977 pKa = 10.2 KK978 pKa = 10.07 LIKK981 pKa = 9.74 AYY983 pKa = 10.36 CSEE986 pKa = 4.21 SVKK989 pKa = 11.04 DD990 pKa = 3.44 KK991 pKa = 9.97 TVQVIPEE998 pKa = 4.19 DD999 pKa = 3.68 QEE1001 pKa = 4.0 EE1002 pKa = 4.18 LSIEE1006 pKa = 4.1 LKK1008 pKa = 9.97 IDD1010 pKa = 3.29 QVNVIDD1016 pKa = 3.72 QDD1018 pKa = 4.27 TIISYY1023 pKa = 10.5 DD1024 pKa = 3.7 DD1025 pKa = 3.83 KK1026 pKa = 11.36 SAHH1029 pKa = 7.2 DD1030 pKa = 4.3 EE1031 pKa = 4.55 NIHH1034 pKa = 6.64 HH1035 pKa = 7.66 ADD1037 pKa = 3.26 TGIATLWDD1045 pKa = 3.87 WIKK1048 pKa = 11.44 APFNWVASFFGSFFDD1063 pKa = 3.68 LVRR1066 pKa = 11.84 VILVILAVIVGLYY1079 pKa = 9.16 ILGYY1083 pKa = 9.82 IYY1085 pKa = 10.83 NLSFSYY1091 pKa = 10.86 YY1092 pKa = 9.06 KK1093 pKa = 10.32 EE1094 pKa = 3.55 KK1095 pKa = 10.64 RR1096 pKa = 11.84 KK1097 pKa = 10.45 RR1098 pKa = 11.84 KK1099 pKa = 8.85 MEE1101 pKa = 4.21 FDD1103 pKa = 3.53 LEE1105 pKa = 4.14 EE1106 pKa = 5.89 AEE1108 pKa = 4.13 EE1109 pKa = 4.06 SLIIKK1114 pKa = 8.8 SNKK1117 pKa = 8.26 YY1118 pKa = 9.23 GEE1120 pKa = 4.27 PRR1122 pKa = 11.84 RR1123 pKa = 11.84 RR1124 pKa = 11.84 TPPKK1128 pKa = 9.63 TFQFPLDD1135 pKa = 3.67 LL1136 pKa = 5.62
Molecular weight: 128.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.919
IPC2_protein 6.071
IPC_protein 6.097
Toseland 6.173
ProMoST 6.517
Dawson 6.453
Bjellqvist 6.478
Wikipedia 6.453
Rodwell 6.44
Grimsley 6.237
Solomon 6.453
Lehninger 6.453
Nozaki 6.751
DTASelect 6.868
Thurlkill 6.883
EMBOSS 6.854
Sillero 6.839
Patrickios 3.948
IPC_peptide 6.465
IPC2_peptide 6.62
IPC2.peptide.svr19 6.68
Protein with the highest isoelectric point:
>tr|Q8V6X1|Q8V6X1_9VIRU Envelopment polyprotein OS=Iris yellow spot virus OX=60456 GN=GP PE=3 SV=1
MM1 pKa = 6.61 STVRR5 pKa = 11.84 VKK7 pKa = 10.67 PSEE10 pKa = 3.94 IEE12 pKa = 3.97 KK13 pKa = 10.48 LLSGGDD19 pKa = 3.19 VDD21 pKa = 5.66 VVIEE25 pKa = 4.06 SDD27 pKa = 3.26 EE28 pKa = 4.39 TEE30 pKa = 4.08 GFNFKK35 pKa = 10.89 NFVLANEE42 pKa = 4.8 GVQMTFNNGYY52 pKa = 8.29 TILRR56 pKa = 11.84 NRR58 pKa = 11.84 AGIYY62 pKa = 8.65 KK63 pKa = 9.27 TIKK66 pKa = 8.24 TGKK69 pKa = 8.6 FTFQGKK75 pKa = 8.15 TIVIPSANVSPNQDD89 pKa = 2.3 DD90 pKa = 3.58 WTFRR94 pKa = 11.84 RR95 pKa = 11.84 LEE97 pKa = 3.93 GFIRR101 pKa = 11.84 ARR103 pKa = 11.84 MLVEE107 pKa = 4.84 LIEE110 pKa = 4.45 TKK112 pKa = 10.46 DD113 pKa = 3.46 EE114 pKa = 4.15 KK115 pKa = 10.84 EE116 pKa = 3.9 KK117 pKa = 11.02 QKK119 pKa = 10.27 MYY121 pKa = 11.02 EE122 pKa = 4.31 KK123 pKa = 10.3 ICGLPLVSAYY133 pKa = 10.15 GLKK136 pKa = 10.34 PSSKK140 pKa = 9.73 FHH142 pKa = 6.3 ATTARR147 pKa = 11.84 IMLTLGGPLTLMASLDD163 pKa = 3.54 IFAAAALPLAYY174 pKa = 9.83 FQNVKK179 pKa = 10.5 KK180 pKa = 9.87 EE181 pKa = 3.81 ALGISRR187 pKa = 11.84 FSTYY191 pKa = 9.83 EE192 pKa = 3.58 QLCKK196 pKa = 10.03 VARR199 pKa = 11.84 VMAAKK204 pKa = 9.95 EE205 pKa = 4.04 FKK207 pKa = 9.89 FTEE210 pKa = 4.36 KK211 pKa = 10.36 YY212 pKa = 10.58 KK213 pKa = 10.91 KK214 pKa = 10.44 IFDD217 pKa = 3.76 EE218 pKa = 4.78 TIKK221 pKa = 10.65 ILTDD225 pKa = 3.56 CTPGTSGAASLIKK238 pKa = 10.52 FNEE241 pKa = 3.98 QIKK244 pKa = 10.11 ILEE247 pKa = 4.29 GAFGKK252 pKa = 10.12 IVEE255 pKa = 5.34 DD256 pKa = 3.22 IGEE259 pKa = 4.16 SSKK262 pKa = 11.19 PKK264 pKa = 9.98 TPSKK268 pKa = 10.28 KK269 pKa = 10.0 DD270 pKa = 3.08 RR271 pKa = 11.84 YY272 pKa = 9.62 NN273 pKa = 3.03
Molecular weight: 30.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.732
IPC2_protein 8.58
IPC_protein 8.463
Toseland 9.37
ProMoST 9.033
Dawson 9.589
Bjellqvist 9.282
Wikipedia 9.706
Rodwell 10.014
Grimsley 9.633
Solomon 9.633
Lehninger 9.604
Nozaki 9.472
DTASelect 9.224
Thurlkill 9.443
EMBOSS 9.78
Sillero 9.545
Patrickios 5.385
IPC_peptide 9.633
IPC2_peptide 7.746
IPC2.peptide.svr19 7.843
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
6483
273
2873
926.1
105.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.795 ± 0.529
2.453 ± 0.373
6.355 ± 0.393
6.185 ± 0.383
4.936 ± 0.24
4.257 ± 0.356
1.512 ± 0.146
7.605 ± 0.16
9.27 ± 0.206
8.808 ± 0.612
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.178 ± 0.538
6.047 ± 0.6
3.039 ± 0.384
2.391 ± 0.09
3.378 ± 0.095
9.918 ± 0.281
5.954 ± 0.205
6.016 ± 0.393
0.848 ± 0.126
4.057 ± 0.34
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here