Mangrovibacterium diazotrophicum

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Marinilabiliales; Prolixibacteraceae; Mangrovibacterium

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4641 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A419VY99|A0A419VY99_9BACT Signal recognition particle protein OS=Mangrovibacterium diazotrophicum OX=1261403 GN=ffh PE=3 SV=1
MM1 pKa = 7.48NNPTTHH7 pKa = 5.78FMKK10 pKa = 10.59VNHH13 pKa = 6.81KK14 pKa = 10.53LLILSLSIGLMTALSCKK31 pKa = 10.33KK32 pKa = 10.43YY33 pKa = 10.95DD34 pKa = 4.88GDD36 pKa = 6.01DD37 pKa = 3.73SDD39 pKa = 6.43DD40 pKa = 5.38DD41 pKa = 5.22DD42 pKa = 7.11DD43 pKa = 3.87EE44 pKa = 4.58TTIVDD49 pKa = 3.93AEE51 pKa = 4.25TGNAEE56 pKa = 4.13DD57 pKa = 4.46HH58 pKa = 6.82EE59 pKa = 4.53EE60 pKa = 4.0ASDD63 pKa = 4.05YY64 pKa = 10.91VWEE67 pKa = 5.14GDD69 pKa = 3.63TVTAIILNEE78 pKa = 3.84TSITVSGDD86 pKa = 2.94GASASGTTATVTAAGTYY103 pKa = 9.5LVSGTLDD110 pKa = 3.12NGKK113 pKa = 10.08IIVDD117 pKa = 4.06TEE119 pKa = 4.24DD120 pKa = 3.23EE121 pKa = 4.39DD122 pKa = 4.66AVKK125 pKa = 10.64LVLNAVNITCSNSAPIFVKK144 pKa = 10.46SAEE147 pKa = 4.07KK148 pKa = 10.49AIVILPSGTTNYY160 pKa = 8.93LTDD163 pKa = 4.11GSSYY167 pKa = 10.94SYY169 pKa = 11.05SSSDD173 pKa = 3.14EE174 pKa = 4.4DD175 pKa = 3.72EE176 pKa = 4.79PNAALYY182 pKa = 10.6SKK184 pKa = 10.89SDD186 pKa = 3.53LSISGEE192 pKa = 3.99GALVVDD198 pKa = 4.85ANYY201 pKa = 11.03NDD203 pKa = 5.37GITSKK208 pKa = 10.79DD209 pKa = 3.32GLIIASGNISVNSVDD224 pKa = 4.3DD225 pKa = 4.77GIRR228 pKa = 11.84GKK230 pKa = 10.4DD231 pKa = 3.41YY232 pKa = 11.33LIIEE236 pKa = 4.68SGTINVTSGGDD247 pKa = 3.56GFKK250 pKa = 10.7SDD252 pKa = 4.99NDD254 pKa = 3.25EE255 pKa = 4.91DD256 pKa = 4.13DD257 pKa = 3.73AKK259 pKa = 11.19GYY261 pKa = 10.46ISIEE265 pKa = 4.16SGTITITAANDD276 pKa = 3.21GMQATTDD283 pKa = 3.91LLIADD288 pKa = 4.05GTFNLTTGGGSSKK301 pKa = 9.69TISSSVSAKK310 pKa = 10.23GLKK313 pKa = 9.74SDD315 pKa = 4.03LNIIIEE321 pKa = 4.52SGDD324 pKa = 3.45FTINSADD331 pKa = 3.9DD332 pKa = 5.08AIHH335 pKa = 5.93SASLITINDD344 pKa = 3.41GTIAISSGDD353 pKa = 3.92DD354 pKa = 4.37GIHH357 pKa = 6.6TDD359 pKa = 2.93NTVNVNGGEE368 pKa = 3.92ITITKK373 pKa = 9.62SYY375 pKa = 10.67EE376 pKa = 4.45GIEE379 pKa = 4.17GPYY382 pKa = 10.35INMNGGTTYY391 pKa = 9.28VTSSDD396 pKa = 3.87DD397 pKa = 3.46GLNASKK403 pKa = 11.33GNGGEE408 pKa = 4.31SNDD411 pKa = 3.6GSLLSITGGYY421 pKa = 10.23LYY423 pKa = 10.21ISSTGDD429 pKa = 3.34GLDD432 pKa = 3.57SNGSITSTGGTVIVNGPSSSVEE454 pKa = 3.63VGLDD458 pKa = 3.46YY459 pKa = 11.63NGTFSLNGGLILIAGPNSSMTQGASSSSTQNSVLVRR495 pKa = 11.84FSSSKK500 pKa = 10.51SSSTLFNIQDD510 pKa = 3.25ASGNSLVTFQPAKK523 pKa = 8.98TYY525 pKa = 11.16SSVLYY530 pKa = 10.56SSSDD534 pKa = 3.36LTKK537 pKa = 9.08GTTYY541 pKa = 10.69YY542 pKa = 9.81IYY544 pKa = 10.95SGGSYY549 pKa = 10.28SGGSEE554 pKa = 3.83TDD556 pKa = 3.13GLYY559 pKa = 10.32TGGTYY564 pKa = 10.33SGGTQYY570 pKa = 11.67SSFTVSGSVTSVGSSSGSSGGGGGGRR596 pKa = 3.79

Molecular weight: 60.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A419VVZ8|A0A419VVZ8_9BACT Iron-only hydrogenase maturation protein HydG OS=Mangrovibacterium diazotrophicum OX=1261403 GN=BC643_4014 PE=4 SV=1
MM1 pKa = 7.28AQIKK5 pKa = 9.38QRR7 pKa = 11.84GVIEE11 pKa = 4.33LGKK14 pKa = 10.04SLLMVVLGLALLFIPEE30 pKa = 4.05VTLVSLARR38 pKa = 11.84LVAIFLIVQGIFVALSAFRR57 pKa = 11.84YY58 pKa = 9.35RR59 pKa = 11.84RR60 pKa = 11.84ADD62 pKa = 3.01INKK65 pKa = 8.97YY66 pKa = 10.85AFGLEE71 pKa = 4.12AGVSLIVGFIILYY84 pKa = 10.41NPGTTISFFVVVLAIWAILGGLLMAFSFNQLRR116 pKa = 11.84KK117 pKa = 10.11AGVTSWFLFFNSLVAMTFGLVLIFEE142 pKa = 4.68PLRR145 pKa = 11.84GGFALASIIGAFVLLHH161 pKa = 6.29GLLSLFSAFSRR172 pKa = 3.95

Molecular weight: 18.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4641

0

4641

1732579

30

6730

373.3

41.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.032 ± 0.036

0.883 ± 0.016

5.818 ± 0.025

6.625 ± 0.032

4.991 ± 0.026

6.862 ± 0.031

1.744 ± 0.019

6.843 ± 0.032

6.444 ± 0.038

9.378 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.412 ± 0.019

5.206 ± 0.028

3.747 ± 0.021

3.699 ± 0.021

4.037 ± 0.027

6.634 ± 0.04

5.518 ± 0.057

6.593 ± 0.033

1.328 ± 0.015

4.204 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski