Achromobacter phage vB_AxyS_19-32_Axy16

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Steinhofvirus; unclassified Steinhofvirus

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A514CV62|A0A514CV62_9CAUD Uncharacterized protein OS=Achromobacter phage vB_AxyS_19-32_Axy16 OX=2591051 GN=Axy16_006 PE=4 SV=1
MM1 pKa = 7.83DD2 pKa = 5.07RR3 pKa = 11.84ADD5 pKa = 4.31IEE7 pKa = 4.23DD8 pKa = 3.79LQRR11 pKa = 11.84VRR13 pKa = 11.84DD14 pKa = 5.11FICNEE19 pKa = 3.72LALTDD24 pKa = 3.89EE25 pKa = 4.44MQLLVSQIEE34 pKa = 3.97LRR36 pKa = 11.84IATIEE41 pKa = 4.03VSIEE45 pKa = 3.78EE46 pKa = 4.21

Molecular weight:
5.38 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A514CV49|A0A514CV49_9CAUD Uncharacterized protein OS=Achromobacter phage vB_AxyS_19-32_Axy16 OX=2591051 GN=Axy16_053 PE=4 SV=1
MM1 pKa = 6.46VTSPKK6 pKa = 10.58LGFTDD11 pKa = 3.67IPAARR16 pKa = 11.84ALALATIEE24 pKa = 4.43QRR26 pKa = 11.84QAGQNVYY33 pKa = 10.15ISNLLPYY40 pKa = 9.98IGRR43 pKa = 11.84LNDD46 pKa = 4.32GYY48 pKa = 11.18SSQAPAGFVEE58 pKa = 4.23NALVVARR65 pKa = 11.84QVARR69 pKa = 11.84NIRR72 pKa = 11.84LLQDD76 pKa = 3.04VGKK79 pKa = 10.28SSRR82 pKa = 11.84RR83 pKa = 11.84SNN85 pKa = 3.17

Molecular weight:
9.2 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

14012

46

1165

241.6

26.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.549 ± 0.379

0.871 ± 0.126

5.681 ± 0.27

6.73 ± 0.283

3.718 ± 0.257

7.85 ± 0.348

1.677 ± 0.201

5.067 ± 0.166

5.317 ± 0.38

8.207 ± 0.26

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.833 ± 0.149

4.182 ± 0.291

5.024 ± 0.269

4.232 ± 0.247

5.852 ± 0.22

5.652 ± 0.205

5.581 ± 0.328

6.873 ± 0.236

1.906 ± 0.232

3.197 ± 0.178

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski