Achromobacter phage vB_AxyS_19-32_Axy16
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A514CV62|A0A514CV62_9CAUD Uncharacterized protein OS=Achromobacter phage vB_AxyS_19-32_Axy16 OX=2591051 GN=Axy16_006 PE=4 SV=1
MM1 pKa = 7.83 DD2 pKa = 5.07 RR3 pKa = 11.84 ADD5 pKa = 4.31 IEE7 pKa = 4.23 DD8 pKa = 3.79 LQRR11 pKa = 11.84 VRR13 pKa = 11.84 DD14 pKa = 5.11 FICNEE19 pKa = 3.72 LALTDD24 pKa = 3.89 EE25 pKa = 4.44 MQLLVSQIEE34 pKa = 3.97 LRR36 pKa = 11.84 IATIEE41 pKa = 4.03 VSIEE45 pKa = 3.78 EE46 pKa = 4.21
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.377
IPC2_protein 4.024
IPC_protein 3.872
Toseland 3.706
ProMoST 3.973
Dawson 3.846
Bjellqvist 4.101
Wikipedia 3.77
Rodwell 3.719
Grimsley 3.617
Solomon 3.821
Lehninger 3.783
Nozaki 3.986
DTASelect 4.113
Thurlkill 3.757
EMBOSS 3.783
Sillero 3.999
Patrickios 3.846
IPC_peptide 3.821
IPC2_peptide 3.973
IPC2.peptide.svr19 3.924
Protein with the highest isoelectric point:
>tr|A0A514CV49|A0A514CV49_9CAUD Uncharacterized protein OS=Achromobacter phage vB_AxyS_19-32_Axy16 OX=2591051 GN=Axy16_053 PE=4 SV=1
MM1 pKa = 6.46 VTSPKK6 pKa = 10.58 LGFTDD11 pKa = 3.67 IPAARR16 pKa = 11.84 ALALATIEE24 pKa = 4.43 QRR26 pKa = 11.84 QAGQNVYY33 pKa = 10.15 ISNLLPYY40 pKa = 9.98 IGRR43 pKa = 11.84 LNDD46 pKa = 4.32 GYY48 pKa = 11.18 SSQAPAGFVEE58 pKa = 4.23 NALVVARR65 pKa = 11.84 QVARR69 pKa = 11.84 NIRR72 pKa = 11.84 LLQDD76 pKa = 3.04 VGKK79 pKa = 10.28 SSRR82 pKa = 11.84 RR83 pKa = 11.84 SNN85 pKa = 3.17
Molecular weight: 9.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.322
IPC2_protein 9.838
IPC_protein 10.789
Toseland 10.526
ProMoST 10.394
Dawson 10.701
Bjellqvist 10.482
Wikipedia 10.979
Rodwell 10.76
Grimsley 10.789
Solomon 10.833
Lehninger 10.774
Nozaki 10.496
DTASelect 10.482
Thurlkill 10.57
EMBOSS 10.95
Sillero 10.628
Patrickios 10.54
IPC_peptide 10.818
IPC2_peptide 9.487
IPC2.peptide.svr19 8.35
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
58
0
58
14012
46
1165
241.6
26.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.549 ± 0.379
0.871 ± 0.126
5.681 ± 0.27
6.73 ± 0.283
3.718 ± 0.257
7.85 ± 0.348
1.677 ± 0.201
5.067 ± 0.166
5.317 ± 0.38
8.207 ± 0.26
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.833 ± 0.149
4.182 ± 0.291
5.024 ± 0.269
4.232 ± 0.247
5.852 ± 0.22
5.652 ± 0.205
5.581 ± 0.328
6.873 ± 0.236
1.906 ± 0.232
3.197 ± 0.178
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here