Streptococcus urinalis 2285-97
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2206 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G5KDT2|G5KDT2_9STRE Ribosomal protein L11 methyltransferase OS=Streptococcus urinalis 2285-97 OX=764291 GN=prmA PE=3 SV=1
MM1 pKa = 7.71 AKK3 pKa = 9.71 EE4 pKa = 4.7 CVWNWLAAIDD14 pKa = 4.23 EE15 pKa = 4.43 KK16 pKa = 11.72 VEE18 pKa = 4.73 DD19 pKa = 4.45 GDD21 pKa = 3.84 ILLISSIRR29 pKa = 11.84 EE30 pKa = 3.64 GLNQVKK36 pKa = 10.21 KK37 pKa = 9.97 NYY39 pKa = 9.62 PGMDD43 pKa = 3.88 FSVSDD48 pKa = 3.84 GADD51 pKa = 3.21 CVGDD55 pKa = 3.55 GWYY58 pKa = 7.34 EE59 pKa = 3.84 TLFFITFPVKK69 pKa = 10.64 DD70 pKa = 3.83 GSDD73 pKa = 3.59 DD74 pKa = 3.41 LRR76 pKa = 11.84 NYY78 pKa = 10.24 CDD80 pKa = 3.35 YY81 pKa = 11.44 DD82 pKa = 4.22 AFVDD86 pKa = 5.68 DD87 pKa = 3.81 VNGWVNHH94 pKa = 5.84 DD95 pKa = 3.88 TLYY98 pKa = 10.41 PIYY101 pKa = 9.32 VTPEE105 pKa = 3.59 FVGEE109 pKa = 4.27 IFDD112 pKa = 3.88 EE113 pKa = 4.54
Molecular weight: 12.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.701
IPC2_protein 3.745
IPC_protein 3.732
Toseland 3.516
ProMoST 3.884
Dawson 3.732
Bjellqvist 3.935
Wikipedia 3.694
Rodwell 3.554
Grimsley 3.427
Solomon 3.719
Lehninger 3.681
Nozaki 3.859
DTASelect 4.101
Thurlkill 3.579
EMBOSS 3.694
Sillero 3.859
Patrickios 0.947
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.801
Protein with the highest isoelectric point:
>tr|G5KH98|G5KH98_9STRE N-acylglucosamine-6-phosphate 2-epimerase OS=Streptococcus urinalis 2285-97 OX=764291 GN=STRUR_0191 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.07 QPSKK9 pKa = 9.67 IRR11 pKa = 11.84 RR12 pKa = 11.84 QRR14 pKa = 11.84 KK15 pKa = 7.52 HH16 pKa = 5.2 GFRR19 pKa = 11.84 HH20 pKa = 6.39 RR21 pKa = 11.84 MSTKK25 pKa = 9.02 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLASRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.73 GRR39 pKa = 11.84 KK40 pKa = 8.76 VLSAA44 pKa = 4.05
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.449
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.398
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.135
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.082
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2206
0
2206
629093
37
2145
285.2
32.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.771 ± 0.071
0.528 ± 0.014
5.71 ± 0.05
6.589 ± 0.061
4.595 ± 0.045
6.221 ± 0.057
1.956 ± 0.026
7.932 ± 0.056
7.54 ± 0.048
9.726 ± 0.069
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.517 ± 0.027
5.105 ± 0.044
3.13 ± 0.026
4.152 ± 0.037
3.552 ± 0.039
6.811 ± 0.055
5.915 ± 0.06
6.599 ± 0.049
0.861 ± 0.018
3.789 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here