Proteome-pI 2.0 - Proteome Isoelectric Point Database

Pseudomonas phage PhiPA3 (Pseudomonas aeruginosa phage PhiPA3)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Phikzvirus; unclassified Phikzvirus

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 375 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|F8SJQ2|F8SJQ2_BPPA3 Virion structural protein OS=Pseudomonas phage PhiPA3 OX=998086 GN=021 PE=4 SV=1

MM1 pKa = 6.83MRR3 pKa = 11.84KK4 pKa = 8.96ILVSVLFALVPCVVQAEE21 pKa = 4.51DD22 pKa = 4.19NPDD25 pKa = 4.24LLTQMYY31 pKa = 10.14LDD33 pKa = 4.6SEE35 pKa = 4.34LVYY38 pKa = 10.58CKK40 pKa = 9.95TGEE43 pKa = 4.3CVVVDD48 pKa = 3.99TGEE51 pKa = 3.96TWEE54 pKa = 4.7QPDD57 pKa = 3.45ITDD60 pKa = 3.48GVYY63 pKa = 10.6SIAFPEE69 pKa = 4.02NKK71 pKa = 8.88YY72 pKa = 9.03TEE74 pKa = 4.05QQIADD79 pKa = 3.98SGPFISDD86 pKa = 2.78AWRR89 pKa = 11.84TRR91 pKa = 11.84IEE93 pKa = 4.07MMEE96 pKa = 4.27RR97 pKa = 11.84DD98 pKa = 4.36CTPPTISQKK107 pKa = 10.11YY108 pKa = 8.93KK109 pKa = 11.47DD110 pKa = 3.81MVGCQQ115 pKa = 2.96

Molecular weight: 13.08 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|F8SJB9|F8SJB9_BPPA3 Uncharacterized protein 283 OS=Pseudomonas phage PhiPA3 OX=998086 GN=283 PE=4 SV=1

MM1 pKa = 7.68SSDD4 pKa = 3.39KK5 pKa = 11.05SKK7 pKa = 10.41KK8 pKa = 8.43QYY10 pKa = 9.8RR11 pKa = 11.84HH12 pKa = 5.37HH13 pKa = 6.92HH14 pKa = 5.19EE15 pKa = 3.91QRR17 pKa = 11.84HH18 pKa = 4.44VKK20 pKa = 9.98AQVAIWRR27 pKa = 11.84FYY29 pKa = 9.18NQQAASLDD37 pKa = 3.58DD38 pKa = 3.58RR39 pKa = 11.84RR40 pKa = 11.84LGRR43 pKa = 11.84MRR45 pKa = 11.84KK46 pKa = 8.01EE47 pKa = 3.5HH48 pKa = 6.85ALNCGRR54 pKa = 11.84SHH56 pKa = 7.73CMLCVNPRR64 pKa = 11.84RR65 pKa = 11.84TWGRR69 pKa = 11.84KK70 pKa = 5.58TLAEE74 pKa = 4.13KK75 pKa = 10.64VADD78 pKa = 3.52IRR80 pKa = 11.84FKK82 pKa = 10.64EE83 pKa = 4.07QQQ85 pKa = 2.94

Molecular weight: 10.24 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
375	0	375	91007	43	2319	242.7	27.43

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
7.504 ± 0.176	0.913 ± 0.047	6.46 ± 0.093	6.523 ± 0.164	4.024 ± 0.099	6.369 ± 0.155	2.176 ± 0.075	6.023 ± 0.092	5.374 ± 0.127	8.465 ± 0.101

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.861 ± 0.082	5.04 ± 0.096	4.213 ± 0.08	4.071 ± 0.086	5.508 ± 0.108	5.6 ± 0.086	6.184 ± 0.123	7.182 ± 0.094	1.449 ± 0.051	4.061 ± 0.106

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski