Amino acid dipepetide frequency for Cyanobium gracile (strain ATCC 27147 / PCC 6307)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.059AlaAla: 18.059 ± 0.237	1.468AlaCys: 1.468 ± 0.04	6.516AlaAsp: 6.516 ± 0.099	7.066AlaGlu: 7.066 ± 0.115	3.427AlaPhe: 3.427 ± 0.062	11.335AlaGly: 11.335 ± 0.156	2.038AlaHis: 2.038 ± 0.049	5.226AlaIle: 5.226 ± 0.079	1.985AlaLys: 1.985 ± 0.052	15.494AlaLeu: 15.494 ± 0.2	2.792AlaMet: 2.792 ± 0.059	2.183AlaAsn: 2.183 ± 0.052	6.836AlaPro: 6.836 ± 0.109	3.646AlaGln: 3.646 ± 0.065	8.153AlaArg: 8.153 ± 0.101	6.76AlaSer: 6.76 ± 0.101	5.756AlaThr: 5.756 ± 0.092	8.915AlaVal: 8.915 ± 0.122	1.885AlaTrp: 1.885 ± 0.046	2.083AlaTyr: 2.083 ± 0.042	0.0AlaXaa: 0.0 ± 0.0
Cys	0.923CysAla: 0.923 ± 0.029	0.2CysCys: 0.2 ± 0.013	0.577CysAsp: 0.577 ± 0.023	0.489CysGlu: 0.489 ± 0.021	0.414CysPhe: 0.414 ± 0.02	1.228CysGly: 1.228 ± 0.035	0.328CysHis: 0.328 ± 0.016	0.341CysIle: 0.341 ± 0.015	0.171CysLys: 0.171 ± 0.014	1.329CysLeu: 1.329 ± 0.044	0.15CysMet: 0.15 ± 0.015	0.245CysAsn: 0.245 ± 0.016	0.728CysPro: 0.728 ± 0.027	0.384CysGln: 0.384 ± 0.021	1.236CysArg: 1.236 ± 0.036	0.706CysSer: 0.706 ± 0.028	0.458CysThr: 0.458 ± 0.023	0.63CysVal: 0.63 ± 0.025	0.263CysTrp: 0.263 ± 0.016	0.197CysTyr: 0.197 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	5.378AspAla: 5.378 ± 0.079	0.507AspCys: 0.507 ± 0.022	2.516AspAsp: 2.516 ± 0.059	2.224AspGlu: 2.224 ± 0.05	1.528AspPhe: 1.528 ± 0.041	5.391AspGly: 5.391 ± 0.09	1.191AspHis: 1.191 ± 0.037	1.513AspIle: 1.513 ± 0.043	0.812AspLys: 0.812 ± 0.03	6.915AspLeu: 6.915 ± 0.094	0.641AspMet: 0.641 ± 0.027	0.95AspAsn: 0.95 ± 0.034	4.67AspPro: 4.67 ± 0.078	1.982AspGln: 1.982 ± 0.048	4.333AspArg: 4.333 ± 0.079	2.144AspSer: 2.144 ± 0.053	2.073AspThr: 2.073 ± 0.051	2.986AspVal: 2.986 ± 0.065	1.034AspTrp: 1.034 ± 0.035	1.038AspTyr: 1.038 ± 0.036	0.0AspXaa: 0.0 ± 0.0
Glu	8.558GluAla: 8.558 ± 0.135	0.343GluCys: 0.343 ± 0.019	2.244GluAsp: 2.244 ± 0.047	3.427GluGlu: 3.427 ± 0.077	1.238GluPhe: 1.238 ± 0.04	4.907GluGly: 4.907 ± 0.068	1.108GluHis: 1.108 ± 0.037	2.08GluIle: 2.08 ± 0.047	1.239GluLys: 1.239 ± 0.048	7.285GluLeu: 7.285 ± 0.101	0.859GluMet: 0.859 ± 0.028	0.979GluAsn: 0.979 ± 0.038	3.4GluPro: 3.4 ± 0.07	2.834GluGln: 2.834 ± 0.063	6.135GluArg: 6.135 ± 0.106	2.441GluSer: 2.441 ± 0.049	2.356GluThr: 2.356 ± 0.046	3.833GluVal: 3.833 ± 0.064	0.709GluTrp: 0.709 ± 0.028	0.603GluTyr: 0.603 ± 0.026	0.0GluXaa: 0.0 ± 0.0
Phe	3.264PheAla: 3.264 ± 0.056	0.447PheCys: 0.447 ± 0.021	2.03PheAsp: 2.03 ± 0.056	1.692PheGlu: 1.692 ± 0.041	1.075PhePhe: 1.075 ± 0.039	2.936PheGly: 2.936 ± 0.063	0.74PheHis: 0.74 ± 0.03	1.088PheIle: 1.088 ± 0.037	0.554PheLys: 0.554 ± 0.026	3.507PheLeu: 3.507 ± 0.071	0.5PheMet: 0.5 ± 0.022	0.926PheAsn: 0.926 ± 0.038	1.555PhePro: 1.555 ± 0.045	1.177PheGln: 1.177 ± 0.036	2.313PheArg: 2.313 ± 0.05	2.251PheSer: 2.251 ± 0.056	1.644PheThr: 1.644 ± 0.06	1.902PheVal: 1.902 ± 0.046	0.568PheTrp: 0.568 ± 0.025	0.703PheTyr: 0.703 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	9.436GlyAla: 9.436 ± 0.126	1.209GlyCys: 1.209 ± 0.041	4.581GlyAsp: 4.581 ± 0.088	4.942GlyGlu: 4.942 ± 0.074	3.282GlyPhe: 3.282 ± 0.056	8.395GlyGly: 8.395 ± 0.159	1.905GlyHis: 1.905 ± 0.043	3.534GlyIle: 3.534 ± 0.064	2.044GlyLys: 2.044 ± 0.051	11.884GlyLeu: 11.884 ± 0.131	1.941GlyMet: 1.941 ± 0.05	1.834GlyAsn: 1.834 ± 0.064	4.817GlyPro: 4.817 ± 0.081	3.436GlyGln: 3.436 ± 0.049	7.405GlyArg: 7.405 ± 0.106	5.747GlySer: 5.747 ± 0.1	4.531GlyThr: 4.531 ± 0.16	6.177GlyVal: 6.177 ± 0.078	1.97GlyTrp: 1.97 ± 0.047	1.869GlyTyr: 1.869 ± 0.05	0.0GlyXaa: 0.0 ± 0.0
His	1.765HisAla: 1.765 ± 0.053	0.331HisCys: 0.331 ± 0.02	1.024HisAsp: 1.024 ± 0.035	0.772HisGlu: 0.772 ± 0.026	0.7HisPhe: 0.7 ± 0.028	2.248HisGly: 2.248 ± 0.056	0.809HisHis: 0.809 ± 0.038	0.597HisIle: 0.597 ± 0.025	0.3HisLys: 0.3 ± 0.018	2.568HisLeu: 2.568 ± 0.052	0.233HisMet: 0.233 ± 0.016	0.431HisAsn: 0.431 ± 0.024	1.859HisPro: 1.859 ± 0.046	0.899HisGln: 0.899 ± 0.031	2.172HisArg: 2.172 ± 0.046	1.015HisSer: 1.015 ± 0.03	0.853HisThr: 0.853 ± 0.033	1.129HisVal: 1.129 ± 0.039	0.497HisTrp: 0.497 ± 0.021	0.496HisTyr: 0.496 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	4.69IleAla: 4.69 ± 0.083	0.469IleCys: 0.469 ± 0.021	2.746IleAsp: 2.746 ± 0.055	2.614IleGlu: 2.614 ± 0.052	1.034IlePhe: 1.034 ± 0.038	3.969IleGly: 3.969 ± 0.074	0.891IleHis: 0.891 ± 0.029	0.996IleIle: 0.996 ± 0.044	0.837IleLys: 0.837 ± 0.035	3.411IleLeu: 3.411 ± 0.061	0.316IleMet: 0.316 ± 0.02	1.12IleAsn: 1.12 ± 0.044	2.36IlePro: 2.36 ± 0.051	1.32IleGln: 1.32 ± 0.039	2.86IleArg: 2.86 ± 0.063	2.399IleSer: 2.399 ± 0.059	2.424IleThr: 2.424 ± 0.061	2.409IleVal: 2.409 ± 0.055	0.468IleTrp: 0.468 ± 0.022	0.748IleTyr: 0.748 ± 0.029	0.0IleXaa: 0.0 ± 0.0
Lys	2.519LysAla: 2.519 ± 0.066	0.124LysCys: 0.124 ± 0.011	0.988LysAsp: 0.988 ± 0.042	1.183LysGlu: 1.183 ± 0.038	0.438LysPhe: 0.438 ± 0.022	1.83LysGly: 1.83 ± 0.047	0.288LysHis: 0.288 ± 0.021	0.723LysIle: 0.723 ± 0.029	0.566LysLys: 0.566 ± 0.029	2.122LysLeu: 2.122 ± 0.053	0.292LysMet: 0.292 ± 0.019	0.401LysAsn: 0.401 ± 0.024	1.226LysPro: 1.226 ± 0.044	0.766LysGln: 0.766 ± 0.028	1.528LysArg: 1.528 ± 0.043	0.929LysSer: 0.929 ± 0.034	0.977LysThr: 0.977 ± 0.037	1.552LysVal: 1.552 ± 0.042	0.172LysTrp: 0.172 ± 0.014	0.34LysTyr: 0.34 ± 0.021	0.0LysXaa: 0.0 ± 0.0
Leu	17.203LeuAla: 17.203 ± 0.199	1.333LeuCys: 1.333 ± 0.038	6.137LeuAsp: 6.137 ± 0.087	8.903LeuGlu: 8.903 ± 0.124	3.469LeuPhe: 3.469 ± 0.065	10.446LeuGly: 10.446 ± 0.114	2.525LeuHis: 2.525 ± 0.059	4.727LeuIle: 4.727 ± 0.072	2.432LeuLys: 2.432 ± 0.056	17.271LeuLeu: 17.271 ± 0.226	2.257LeuMet: 2.257 ± 0.054	2.502LeuAsn: 2.502 ± 0.055	8.255LeuPro: 8.255 ± 0.109	5.578LeuGln: 5.578 ± 0.091	10.259LeuArg: 10.259 ± 0.13	6.801LeuSer: 6.801 ± 0.101	5.763LeuThr: 5.763 ± 0.075	9.751LeuVal: 9.751 ± 0.118	1.957LeuTrp: 1.957 ± 0.045	1.942LeuTyr: 1.942 ± 0.053	0.0LeuXaa: 0.0 ± 0.0
Met	3.094MetAla: 3.094 ± 0.058	0.085MetCys: 0.085 ± 0.009	0.806MetAsp: 0.806 ± 0.03	1.102MetGlu: 1.102 ± 0.039	0.331MetPhe: 0.331 ± 0.019	1.79MetGly: 1.79 ± 0.047	0.295MetHis: 0.295 ± 0.019	0.605MetIle: 0.605 ± 0.021	0.449MetLys: 0.449 ± 0.024	1.763MetLeu: 1.763 ± 0.049	0.25MetMet: 0.25 ± 0.02	0.47MetAsn: 0.47 ± 0.022	1.123MetPro: 1.123 ± 0.037	0.619MetGln: 0.619 ± 0.022	1.146MetArg: 1.146 ± 0.032	0.963MetSer: 0.963 ± 0.03	1.126MetThr: 1.126 ± 0.034	1.571MetVal: 1.571 ± 0.039	0.1MetTrp: 0.1 ± 0.011	0.181MetTyr: 0.181 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	2.215AsnAla: 2.215 ± 0.058	0.233AsnCys: 0.233 ± 0.016	1.103AsnAsp: 1.103 ± 0.039	0.81AsnGlu: 0.81 ± 0.032	0.68AsnPhe: 0.68 ± 0.04	2.083AsnGly: 2.083 ± 0.082	0.442AsnHis: 0.442 ± 0.021	0.755AsnIle: 0.755 ± 0.031	0.463AsnLys: 0.463 ± 0.023	2.599AsnLeu: 2.599 ± 0.068	0.264AsnMet: 0.264 ± 0.019	0.554AsnAsn: 0.554 ± 0.039	1.655AsnPro: 1.655 ± 0.05	0.814AsnGln: 0.814 ± 0.031	1.777AsnArg: 1.777 ± 0.043	1.049AsnSer: 1.049 ± 0.04	1.018AsnThr: 1.018 ± 0.05	1.28AsnVal: 1.28 ± 0.038	0.308AsnTrp: 0.308 ± 0.02	0.45AsnTyr: 0.45 ± 0.024	0.0AsnXaa: 0.0 ± 0.0
Pro	8.647ProAla: 8.647 ± 0.139	0.628ProCys: 0.628 ± 0.027	3.475ProAsp: 3.475 ± 0.053	4.433ProGlu: 4.433 ± 0.079	2.013ProPhe: 2.013 ± 0.044	5.988ProGly: 5.988 ± 0.096	1.15ProHis: 1.15 ± 0.034	2.185ProIle: 2.185 ± 0.053	0.88ProLys: 0.88 ± 0.034	8.278ProLeu: 8.278 ± 0.126	1.405ProMet: 1.405 ± 0.038	1.012ProAsn: 1.012 ± 0.037	4.534ProPro: 4.534 ± 0.085	2.12ProGln: 2.12 ± 0.047	3.912ProArg: 3.912 ± 0.063	3.783ProSer: 3.783 ± 0.069	2.958ProThr: 2.958 ± 0.059	4.717ProVal: 4.717 ± 0.074	1.252ProTrp: 1.252 ± 0.04	1.047ProTyr: 1.047 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	5.082GlnAla: 5.082 ± 0.087	0.29GlnCys: 0.29 ± 0.018	1.448GlnAsp: 1.448 ± 0.039	2.366GlnGlu: 2.366 ± 0.063	0.957GlnPhe: 0.957 ± 0.034	3.391GlnGly: 3.391 ± 0.067	0.751GlnHis: 0.751 ± 0.028	1.414GlnIle: 1.414 ± 0.04	0.861GlnLys: 0.861 ± 0.031	5.521GlnLeu: 5.521 ± 0.085	0.639GlnMet: 0.639 ± 0.025	0.658GlnAsn: 0.658 ± 0.027	2.671GlnPro: 2.671 ± 0.055	2.206GlnGln: 2.206 ± 0.062	4.046GlnArg: 4.046 ± 0.08	1.731GlnSer: 1.731 ± 0.043	1.344GlnThr: 1.344 ± 0.037	3.042GlnVal: 3.042 ± 0.06	0.66GlnTrp: 0.66 ± 0.024	0.474GlnTyr: 0.474 ± 0.022	0.0GlnXaa: 0.0 ± 0.0
Arg	7.102ArgAla: 7.102 ± 0.1	1.051ArgCys: 1.051 ± 0.033	3.517ArgAsp: 3.517 ± 0.072	4.104ArgGlu: 4.104 ± 0.078	3.192ArgPhe: 3.192 ± 0.055	5.525ArgGly: 5.525 ± 0.073	2.155ArgHis: 2.155 ± 0.045	3.432ArgIle: 3.432 ± 0.053	1.382ArgLys: 1.382 ± 0.04	11.812ArgLeu: 11.812 ± 0.127	1.53ArgMet: 1.53 ± 0.038	1.56ArgAsn: 1.56 ± 0.045	5.221ArgPro: 5.221 ± 0.082	4.515ArgGln: 4.515 ± 0.082	8.593ArgArg: 8.593 ± 0.12	4.987ArgSer: 4.987 ± 0.081	3.189ArgThr: 3.189 ± 0.053	4.931ArgVal: 4.931 ± 0.072	1.969ArgTrp: 1.969 ± 0.042	1.625ArgTyr: 1.625 ± 0.044	0.0ArgXaa: 0.0 ± 0.0
Ser	5.669SerAla: 5.669 ± 0.084	0.656SerCys: 0.656 ± 0.025	2.863SerAsp: 2.863 ± 0.054	2.75SerGlu: 2.75 ± 0.057	2.108SerPhe: 2.108 ± 0.055	5.946SerGly: 5.946 ± 0.112	1.247SerHis: 1.247 ± 0.041	2.157SerIle: 2.157 ± 0.053	1.033SerLys: 1.033 ± 0.033	7.416SerLeu: 7.416 ± 0.099	1.022SerMet: 1.022 ± 0.028	1.196SerAsn: 1.196 ± 0.041	3.689SerPro: 3.689 ± 0.062	2.003SerGln: 2.003 ± 0.054	4.333SerArg: 4.333 ± 0.06	3.584SerSer: 3.584 ± 0.075	2.716SerThr: 2.716 ± 0.06	3.48SerVal: 3.48 ± 0.066	0.995SerTrp: 0.995 ± 0.032	1.156SerTyr: 1.156 ± 0.035	0.0SerXaa: 0.0 ± 0.0
Thr	5.875ThrAla: 5.875 ± 0.102	0.436ThrCys: 0.436 ± 0.024	2.237ThrAsp: 2.237 ± 0.047	1.957ThrGlu: 1.957 ± 0.044	1.483ThrPhe: 1.483 ± 0.057	4.508ThrGly: 4.508 ± 0.091	0.868ThrHis: 0.868 ± 0.035	2.122ThrIle: 2.122 ± 0.078	0.829ThrLys: 0.829 ± 0.033	5.864ThrLeu: 5.864 ± 0.095	0.789ThrMet: 0.789 ± 0.027	1.019ThrAsn: 1.019 ± 0.052	3.742ThrPro: 3.742 ± 0.076	1.19ThrGln: 1.19 ± 0.038	2.757ThrArg: 2.757 ± 0.059	2.712ThrSer: 2.712 ± 0.06	2.654ThrThr: 2.654 ± 0.074	3.805ThrVal: 3.805 ± 0.102	0.67ThrTrp: 0.67 ± 0.028	1.041ThrTyr: 1.041 ± 0.034	0.0ThrXaa: 0.0 ± 0.0
Val	8.874ValAla: 8.874 ± 0.097	0.701ValCys: 0.701 ± 0.026	3.404ValAsp: 3.404 ± 0.062	4.09ValGlu: 4.09 ± 0.068	2.322ValPhe: 2.322 ± 0.056	5.749ValGly: 5.749 ± 0.076	1.241ValHis: 1.241 ± 0.037	3.208ValIle: 3.208 ± 0.066	1.411ValLys: 1.411 ± 0.04	9.559ValLeu: 9.559 ± 0.102	1.423ValMet: 1.423 ± 0.044	1.64ValAsn: 1.64 ± 0.052	4.085ValPro: 4.085 ± 0.069	2.411ValGln: 2.411 ± 0.044	4.948ValArg: 4.948 ± 0.074	3.966ValSer: 3.966 ± 0.072	3.329ValThr: 3.329 ± 0.083	6.357ValVal: 6.357 ± 0.101	0.884ValTrp: 0.884 ± 0.031	1.093ValTyr: 1.093 ± 0.036	0.0ValXaa: 0.0 ± 0.0
Trp	1.385TrpAla: 1.385 ± 0.046	0.256TrpCys: 0.256 ± 0.014	0.669TrpAsp: 0.669 ± 0.026	0.791TrpGlu: 0.791 ± 0.025	0.565TrpPhe: 0.565 ± 0.026	1.265TrpGly: 1.265 ± 0.033	0.39TrpHis: 0.39 ± 0.017	0.798TrpIle: 0.798 ± 0.028	0.382TrpLys: 0.382 ± 0.02	2.754TrpLeu: 2.754 ± 0.06	0.343TrpMet: 0.343 ± 0.02	0.419TrpAsn: 0.419 ± 0.021	0.977TrpPro: 0.977 ± 0.034	0.995TrpGln: 0.995 ± 0.029	1.704TrpArg: 1.704 ± 0.043	1.077TrpSer: 1.077 ± 0.034	0.608TrpThr: 0.608 ± 0.024	0.99TrpVal: 0.99 ± 0.031	0.413TrpTrp: 0.413 ± 0.021	0.296TrpTyr: 0.296 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.887TyrAla: 1.887 ± 0.044	0.23TyrCys: 0.23 ± 0.015	0.949TyrAsp: 0.949 ± 0.032	0.859TyrGlu: 0.859 ± 0.031	0.616TyrPhe: 0.616 ± 0.027	2.012TyrGly: 2.012 ± 0.042	0.334TyrHis: 0.334 ± 0.018	0.58TyrIle: 0.58 ± 0.024	0.332TyrLys: 0.332 ± 0.017	2.16TyrLeu: 2.16 ± 0.058	0.222TyrMet: 0.222 ± 0.013	0.453TyrAsn: 0.453 ± 0.023	1.016TyrPro: 1.016 ± 0.029	0.658TyrGln: 0.658 ± 0.024	1.781TyrArg: 1.781 ± 0.044	0.973TyrSer: 0.973 ± 0.031	0.782TyrThr: 0.782 ± 0.037	1.237TyrVal: 1.237 ± 0.042	0.3TyrTrp: 0.3 ± 0.018	0.398TyrTyr: 0.398 ± 0.022	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3261 proteins (987198 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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