Sanxia water strider virus 15

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 9.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KG98|A0A1L3KG98_9VIRU RNA-directed RNA polymerase OS=Sanxia water strider virus 15 OX=1923399 PE=4 SV=1
MM1 pKa = 7.51SNYY4 pKa = 10.21AINILEE10 pKa = 4.44CNSLPSSPVGHH21 pKa = 6.54MEE23 pKa = 3.56TAKK26 pKa = 10.22WYY28 pKa = 9.92DD29 pKa = 3.56CMRR32 pKa = 11.84MFRR35 pKa = 11.84APRR38 pKa = 11.84RR39 pKa = 11.84WTRR42 pKa = 11.84SLKK45 pKa = 10.85APAAPKK51 pKa = 8.58WVKK54 pKa = 10.34PKK56 pKa = 9.78WSSLAKK62 pKa = 9.33PFKK65 pKa = 10.1RR66 pKa = 11.84VKK68 pKa = 8.91TPKK71 pKa = 9.93RR72 pKa = 11.84EE73 pKa = 3.97PGKK76 pKa = 9.22VTEE79 pKa = 4.27EE80 pKa = 3.99SPYY83 pKa = 10.0PANLSPEE90 pKa = 3.91SRR92 pKa = 11.84TWLTANPTGVTAAIRR107 pKa = 11.84VLNAPSKK114 pKa = 10.19DD115 pKa = 3.59AQPKK119 pKa = 7.61YY120 pKa = 10.61LKK122 pKa = 10.32SALMYY127 pKa = 10.73DD128 pKa = 4.31RR129 pKa = 11.84LCIGHH134 pKa = 7.32DD135 pKa = 3.2RR136 pKa = 11.84LIPNTTEE143 pKa = 3.7FRR145 pKa = 11.84WPNEE149 pKa = 3.57RR150 pKa = 11.84VRR152 pKa = 11.84LCGLIPVHH160 pKa = 7.19RR161 pKa = 11.84IAKK164 pKa = 8.53LHH166 pKa = 4.82NHH168 pKa = 5.05KK169 pKa = 10.48HH170 pKa = 5.5EE171 pKa = 5.14DD172 pKa = 3.14LYY174 pKa = 11.23QYY176 pKa = 11.38LRR178 pKa = 11.84IKK180 pKa = 10.64SMYY183 pKa = 10.8AMRR186 pKa = 11.84SPLLLKK192 pKa = 9.84QLKK195 pKa = 8.97QHH197 pKa = 6.51ALQWLEE203 pKa = 3.94GFDD206 pKa = 3.54TSEE209 pKa = 3.86YY210 pKa = 9.91TPAGLADD217 pKa = 5.43LISRR221 pKa = 11.84TCAQAYY227 pKa = 10.28AGDD230 pKa = 4.17AFDD233 pKa = 4.5ITLEE237 pKa = 4.07TYY239 pKa = 8.51MAEE242 pKa = 4.11RR243 pKa = 11.84QGLHH247 pKa = 6.51PSPSSFHH254 pKa = 6.14HH255 pKa = 6.79RR256 pKa = 11.84PYY258 pKa = 9.99SWRR261 pKa = 11.84RR262 pKa = 11.84VYY264 pKa = 10.96AWLRR268 pKa = 11.84IMKK271 pKa = 10.03LEE273 pKa = 4.19LSSAVFKK280 pKa = 10.67RR281 pKa = 11.84IPPLVCNNRR290 pKa = 11.84TMYY293 pKa = 11.12LDD295 pKa = 3.73LHH297 pKa = 5.99SHH299 pKa = 5.36SFGYY303 pKa = 10.03RR304 pKa = 11.84VGLHH308 pKa = 6.12SSCICNEE315 pKa = 4.03IIAISNRR322 pKa = 11.84HH323 pKa = 6.17CIEE326 pKa = 3.88NPLRR330 pKa = 11.84TSRR333 pKa = 11.84QFDD336 pKa = 3.73VYY338 pKa = 11.19AEE340 pKa = 4.06EE341 pKa = 4.23SVKK344 pKa = 10.5FFNFTIQKK352 pKa = 8.91ISKK355 pKa = 8.65EE356 pKa = 4.23EE357 pKa = 3.94VISRR361 pKa = 11.84YY362 pKa = 8.51TGLKK366 pKa = 7.78KK367 pKa = 10.33SRR369 pKa = 11.84YY370 pKa = 8.54IAACTTLRR378 pKa = 11.84EE379 pKa = 4.27QGWHH383 pKa = 6.53KK384 pKa = 10.74FNTHH388 pKa = 5.38ISSFVKK394 pKa = 10.18VDD396 pKa = 3.13KK397 pKa = 11.06APYY400 pKa = 9.89GKK402 pKa = 10.45LFTKK406 pKa = 10.4APRR409 pKa = 11.84LIQGRR414 pKa = 11.84TPEE417 pKa = 4.07YY418 pKa = 10.03NLCYY422 pKa = 9.62LQYY425 pKa = 9.96VVPMEE430 pKa = 3.9HH431 pKa = 6.12TLYY434 pKa = 11.11EE435 pKa = 4.05NLTYY439 pKa = 10.66GSHH442 pKa = 6.36SNLRR446 pKa = 11.84CVAKK450 pKa = 10.45GLNPQEE456 pKa = 4.1RR457 pKa = 11.84AEE459 pKa = 4.41LYY461 pKa = 9.61LGKK464 pKa = 10.01VASYY468 pKa = 10.06IDD470 pKa = 3.61PVVYY474 pKa = 10.72SGDD477 pKa = 3.43HH478 pKa = 5.16KK479 pKa = 10.03TFDD482 pKa = 3.44AFITVHH488 pKa = 5.96HH489 pKa = 6.9LKK491 pKa = 10.42AVHH494 pKa = 5.8RR495 pKa = 11.84KK496 pKa = 6.62YY497 pKa = 11.04RR498 pKa = 11.84KK499 pKa = 7.64MCGRR503 pKa = 11.84GIKK506 pKa = 9.83RR507 pKa = 11.84YY508 pKa = 9.64CHH510 pKa = 6.14AQLHH514 pKa = 5.76NKK516 pKa = 9.15CKK518 pKa = 8.48TVNGIRR524 pKa = 11.84YY525 pKa = 8.0NIQGTRR531 pKa = 11.84MSGDD535 pKa = 3.49ADD537 pKa = 3.55TGLGNTIISLDD548 pKa = 3.54LAYY551 pKa = 9.37ATLRR555 pKa = 11.84SNNIEE560 pKa = 4.77KK561 pKa = 10.72YY562 pKa = 10.59DD563 pKa = 4.16LLCDD567 pKa = 4.19GDD569 pKa = 4.27DD570 pKa = 3.62FMIIVEE576 pKa = 4.78RR577 pKa = 11.84GTILKK582 pKa = 10.61GDD584 pKa = 3.58MFLPFGFRR592 pKa = 11.84TEE594 pKa = 4.15LTQTEE599 pKa = 3.97VDD601 pKa = 3.3MVEE604 pKa = 4.66FCQCRR609 pKa = 11.84IVDD612 pKa = 3.48TGTRR616 pKa = 11.84QVFVRR621 pKa = 11.84NPEE624 pKa = 3.92RR625 pKa = 11.84MLSNSRR631 pKa = 11.84ICRR634 pKa = 11.84KK635 pKa = 10.09AYY637 pKa = 8.58TVQLYY642 pKa = 10.04KK643 pKa = 10.81EE644 pKa = 4.2WLNSVGLCEE653 pKa = 3.77ATLYY657 pKa = 11.26GDD659 pKa = 3.26IPIYY663 pKa = 9.74STYY666 pKa = 10.4SWSQITDD673 pKa = 3.18RR674 pKa = 11.84TACIKK679 pKa = 11.0DD680 pKa = 3.33PDD682 pKa = 3.68MLRR685 pKa = 11.84RR686 pKa = 11.84MEE688 pKa = 4.43GMPIKK693 pKa = 10.5PIDD696 pKa = 3.72TPVTDD701 pKa = 3.63EE702 pKa = 4.65ARR704 pKa = 11.84VSFYY708 pKa = 10.84KK709 pKa = 10.6VWGVPPEE716 pKa = 3.89IQIEE720 pKa = 4.36LEE722 pKa = 4.18SQLTCTNIFSKK733 pKa = 10.05RR734 pKa = 11.84TIKK737 pKa = 10.4SRR739 pKa = 11.84YY740 pKa = 8.32GPCSGQKK747 pKa = 8.54EE748 pKa = 4.14QPLAEE753 pKa = 4.73LSCSSWWSRR762 pKa = 11.84CEE764 pKa = 3.97CGHH767 pKa = 6.75

Molecular weight: 88.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KG98|A0A1L3KG98_9VIRU RNA-directed RNA polymerase OS=Sanxia water strider virus 15 OX=1923399 PE=4 SV=1
MM1 pKa = 7.95DD2 pKa = 5.13LARR5 pKa = 11.84VRR7 pKa = 11.84KK8 pKa = 9.95SNPLLNLAAAAGGAAANVAINKK30 pKa = 8.12VADD33 pKa = 3.86MMRR36 pKa = 11.84PTPPPRR42 pKa = 11.84NARR45 pKa = 11.84RR46 pKa = 11.84QPQMTVAVPRR56 pKa = 11.84NQPRR60 pKa = 11.84RR61 pKa = 11.84RR62 pKa = 11.84KK63 pKa = 9.59QRR65 pKa = 11.84ASNSNNNGLSMARR78 pKa = 11.84QITPGSSIRR87 pKa = 11.84VVDD90 pKa = 3.69TEE92 pKa = 4.26YY93 pKa = 10.54LTVPSTTLNQMQFNPAPPDD112 pKa = 3.49LARR115 pKa = 11.84LEE117 pKa = 4.51QFSKK121 pKa = 9.69MYY123 pKa = 10.46HH124 pKa = 5.38RR125 pKa = 11.84FKK127 pKa = 10.61INRR130 pKa = 11.84ITVHH134 pKa = 5.72YY135 pKa = 9.0EE136 pKa = 3.58PGVGTATTGNISMGICVGPPLGNITTSAHH165 pKa = 5.36IMKK168 pKa = 8.36LTPSCFVPAWKK179 pKa = 10.22SGSITVGRR187 pKa = 11.84DD188 pKa = 2.7IDD190 pKa = 3.11IGRR193 pKa = 11.84YY194 pKa = 6.9MICGGTGDD202 pKa = 3.83TSVACTLYY210 pKa = 10.48TLASAANLGLFRR222 pKa = 11.84VTYY225 pKa = 9.79DD226 pKa = 3.0VSFSYY231 pKa = 10.31PVNFF235 pKa = 4.45

Molecular weight: 25.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1002

235

767

501.0

56.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.886 ± 1.449

2.994 ± 0.645

3.892 ± 0.244

4.491 ± 1.605

3.194 ± 0.107

5.489 ± 0.871

2.794 ± 0.758

5.888 ± 0.178

5.589 ± 1.303

8.184 ± 0.899

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.994 ± 0.63

4.691 ± 1.27

6.188 ± 0.947

3.194 ± 0.105

7.685 ± 0.2

7.285 ± 0.026

6.886 ± 0.811

5.489 ± 0.871

1.597 ± 0.585

4.591 ± 0.805
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski