Southern bean mosaic virus
Average proteome isoelectric point is 7.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q7T5V6|Q7T5V6_9VIRU Capsid protein OS=Southern bean mosaic virus OX=12139 PE=3 SV=1
MM1 pKa = 6.78 YY2 pKa = 10.23 HH3 pKa = 7.46 PGRR6 pKa = 11.84 SPSFLITLANVICAAILYY24 pKa = 7.55 DD25 pKa = 3.43 IRR27 pKa = 11.84 MGGYY31 pKa = 9.36 QPGSLVPIVAWMTPFVTLLWLSASFVTYY59 pKa = 9.74 LYY61 pKa = 10.18 RR62 pKa = 11.84 YY63 pKa = 9.65 ARR65 pKa = 11.84 TRR67 pKa = 11.84 LLPEE71 pKa = 3.85 EE72 pKa = 4.09 KK73 pKa = 10.13 VARR76 pKa = 11.84 VYY78 pKa = 9.3 YY79 pKa = 8.64 TAQSAPYY86 pKa = 8.85 FDD88 pKa = 4.77 PALGVMMQFAPSHH101 pKa = 6.09 GGASIEE107 pKa = 4.27 VQVNPSWISLLGGSLKK123 pKa = 10.69 INGDD127 pKa = 3.65 DD128 pKa = 4.16 ASNEE132 pKa = 4.26 SAVLGSFYY140 pKa = 11.12 SSVKK144 pKa = 10.53 PGDD147 pKa = 3.72 EE148 pKa = 4.01 PASLVAIKK156 pKa = 10.46 SGPQTIGFGCRR167 pKa = 11.84 TKK169 pKa = 10.47 IDD171 pKa = 3.73 GDD173 pKa = 3.9 DD174 pKa = 4.1 CLFTDD179 pKa = 3.15 NHH181 pKa = 5.66 VWNNSMRR188 pKa = 11.84 PTALAKK194 pKa = 10.35 AGKK197 pKa = 9.23 QVAIEE202 pKa = 4.41 DD203 pKa = 3.64 WDD205 pKa = 4.24 TPLSCDD211 pKa = 3.38 HH212 pKa = 6.88 KK213 pKa = 10.2 MLDD216 pKa = 3.71 FVVVRR221 pKa = 11.84 VPKK224 pKa = 10.45 HH225 pKa = 4.37 VWSKK229 pKa = 11.12 LGVKK233 pKa = 8.63 ATQLVCPSDD242 pKa = 3.81 KK243 pKa = 11.17 DD244 pKa = 4.1 AVTCYY249 pKa = 10.25 GGSSSDD255 pKa = 3.6 SLLSGTGVCSKK266 pKa = 10.96 VDD268 pKa = 3.98 FSWKK272 pKa = 8.5 LTHH275 pKa = 6.57 SCPTAAGWSGTPIYY289 pKa = 10.37 SSRR292 pKa = 11.84 GVVGMHH298 pKa = 6.33 VGFEE302 pKa = 4.97 DD303 pKa = 3.14 IGKK306 pKa = 9.97 LNRR309 pKa = 11.84 GVNAFYY315 pKa = 10.97 VSNYY319 pKa = 9.68 LLRR322 pKa = 11.84 SQEE325 pKa = 4.16 TLPPDD330 pKa = 3.8 LSVIEE335 pKa = 4.5 IPFEE339 pKa = 4.26 DD340 pKa = 4.14 VEE342 pKa = 4.42 TRR344 pKa = 11.84 SYY346 pKa = 11.47 EE347 pKa = 4.17 FIEE350 pKa = 4.21 VEE352 pKa = 3.54 IKK354 pKa = 10.94 GRR356 pKa = 11.84 GKK358 pKa = 10.49 AKK360 pKa = 9.95 LGKK363 pKa = 9.86 RR364 pKa = 11.84 EE365 pKa = 3.99 FAWIPEE371 pKa = 4.18 SGKK374 pKa = 10.41 YY375 pKa = 8.02 WADD378 pKa = 3.92 DD379 pKa = 4.49 DD380 pKa = 5.96 DD381 pKa = 6.55 DD382 pKa = 5.06 SLPPPPKK389 pKa = 10.4 VVDD392 pKa = 4.12 GKK394 pKa = 9.1 MVWTSAQEE402 pKa = 4.18 TVAEE406 pKa = 4.27 PLNLPEE412 pKa = 4.49 GGRR415 pKa = 11.84 VKK417 pKa = 10.81 ALAALSQLAGYY428 pKa = 8.62 NFKK431 pKa = 10.68 EE432 pKa = 4.49 GEE434 pKa = 4.1 AASTRR439 pKa = 11.84 GMPLRR444 pKa = 11.84 FVGQSACKK452 pKa = 9.92 FRR454 pKa = 11.84 EE455 pKa = 4.33 LCRR458 pKa = 11.84 KK459 pKa = 7.66 DD460 pKa = 3.48 TPDD463 pKa = 2.98 EE464 pKa = 3.98 VLRR467 pKa = 11.84 ATRR470 pKa = 11.84 VFPEE474 pKa = 4.61 LSDD477 pKa = 3.9 FSWPEE482 pKa = 3.65 RR483 pKa = 11.84 GSKK486 pKa = 10.82 AEE488 pKa = 3.92 LHH490 pKa = 6.42 SLLLQAGKK498 pKa = 10.22 FNPTAIPRR506 pKa = 11.84 NLEE509 pKa = 3.9 GACQNLLEE517 pKa = 5.14 RR518 pKa = 11.84 YY519 pKa = 7.28 PASKK523 pKa = 10.08 SCYY526 pKa = 8.82 CLRR529 pKa = 11.84 GEE531 pKa = 4.39 AWSFDD536 pKa = 3.22 AVYY539 pKa = 11.09 EE540 pKa = 4.32 EE541 pKa = 4.65 VCKK544 pKa = 10.41 KK545 pKa = 10.59 AQSAEE550 pKa = 4.12 INEE553 pKa = 4.4 KK554 pKa = 10.57 ASPGVPLSRR563 pKa = 11.84 LASTNKK569 pKa = 10.22 DD570 pKa = 3.04 LLKK573 pKa = 10.72 RR574 pKa = 11.84 HH575 pKa = 6.36 LEE577 pKa = 3.98 LVALCVTEE585 pKa = 5.02 RR586 pKa = 11.84 LFLLSEE592 pKa = 4.9 AEE594 pKa = 4.09 DD595 pKa = 3.73 LHH597 pKa = 6.74 NKK599 pKa = 10.05 SPVDD603 pKa = 3.8 LVQMGLCDD611 pKa = 3.77 PVRR614 pKa = 11.84 LFVKK618 pKa = 10.09 QEE620 pKa = 3.55 PHH622 pKa = 6.66 ASRR625 pKa = 11.84 KK626 pKa = 8.72 VKK628 pKa = 10.06 EE629 pKa = 3.96 GRR631 pKa = 11.84 FRR633 pKa = 11.84 LISSVSLVDD642 pKa = 3.53 QLVEE646 pKa = 4.17 RR647 pKa = 11.84 MLFGPQNQLEE657 pKa = 4.27 IAEE660 pKa = 4.39 WEE662 pKa = 4.59 HH663 pKa = 7.05 IPSKK667 pKa = 10.61 PGMGLSLQRR676 pKa = 11.84 QAKK679 pKa = 10.16 SLFDD683 pKa = 4.03 DD684 pKa = 4.2 LRR686 pKa = 11.84 VKK688 pKa = 10.81 HH689 pKa = 5.85 SRR691 pKa = 11.84 CPAAEE696 pKa = 3.96 ADD698 pKa = 3.14 ISGFDD703 pKa = 3.43 WSVQDD708 pKa = 3.34 WEE710 pKa = 4.28 LWADD714 pKa = 3.34 VEE716 pKa = 4.29 MRR718 pKa = 11.84 IVLGGFGQKK727 pKa = 10.49 LSIAARR733 pKa = 11.84 NRR735 pKa = 11.84 FSCFMNSVFQLSDD748 pKa = 3.05 GTLIEE753 pKa = 4.06 QQLPGIMKK761 pKa = 10.12 SGSYY765 pKa = 8.61 CTSSTNSRR773 pKa = 11.84 IRR775 pKa = 11.84 CLMAEE780 pKa = 4.85 LIGSPWCIAMGDD792 pKa = 3.8 DD793 pKa = 5.11 SVEE796 pKa = 4.01 GWVDD800 pKa = 3.51 GAKK803 pKa = 10.31 DD804 pKa = 2.83 KK805 pKa = 11.33 YY806 pKa = 9.83 MRR808 pKa = 11.84 LGHH811 pKa = 5.36 TCKK814 pKa = 10.33 DD815 pKa = 3.75 YY816 pKa = 11.17 KK817 pKa = 10.73 PCATSISGRR826 pKa = 11.84 LYY828 pKa = 9.38 EE829 pKa = 5.04 VEE831 pKa = 4.31 FCSHH835 pKa = 7.24 VIRR838 pKa = 11.84 EE839 pKa = 4.21 DD840 pKa = 3.82 RR841 pKa = 11.84 CWLASWPKK849 pKa = 9.39 TLYY852 pKa = 10.46 KK853 pKa = 10.46 YY854 pKa = 10.66 LSEE857 pKa = 4.23 GKK859 pKa = 9.12 WFFEE863 pKa = 4.14 DD864 pKa = 4.39 LEE866 pKa = 5.46 RR867 pKa = 11.84 EE868 pKa = 4.45 LGSSPHH874 pKa = 6.03 WPRR877 pKa = 11.84 IRR879 pKa = 11.84 HH880 pKa = 4.43 YY881 pKa = 11.18 VVGNTPSPDD890 pKa = 2.95 KK891 pKa = 10.92 TRR893 pKa = 11.84 LEE895 pKa = 4.23 NSSPSYY901 pKa = 11.07 GEE903 pKa = 4.2 EE904 pKa = 3.8 ADD906 pKa = 3.77 KK907 pKa = 8.87 TTVSQGYY914 pKa = 8.23 SEE916 pKa = 5.34 HH917 pKa = 6.7 SGSPGHH923 pKa = 6.54 SIEE926 pKa = 4.28 EE927 pKa = 4.08 AQEE930 pKa = 4.07 PEE932 pKa = 4.2 TAPFCCKK939 pKa = 10.07 AASVYY944 pKa = 10.02 PGWGIHH950 pKa = 6.47 GPYY953 pKa = 10.04 CSGGYY958 pKa = 10.45 GSLTT962 pKa = 3.36
Molecular weight: 106.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.645
IPC2_protein 5.703
IPC_protein 5.741
Toseland 5.982
ProMoST 6.059
Dawson 5.97
Bjellqvist 5.995
Wikipedia 5.957
Rodwell 5.944
Grimsley 6.071
Solomon 5.97
Lehninger 5.957
Nozaki 6.224
DTASelect 6.402
Thurlkill 6.389
EMBOSS 6.351
Sillero 6.326
Patrickios 3.986
IPC_peptide 5.982
IPC2_peptide 6.3
IPC2.peptide.svr19 6.29
Protein with the highest isoelectric point:
>tr|Q7T5V7|Q7T5V7_9VIRU Putative movement protein OS=Southern bean mosaic virus OX=12139 PE=4 SV=1
MM1 pKa = 7.87 AKK3 pKa = 10.33 RR4 pKa = 11.84 LTKK7 pKa = 10.22 QQLAKK12 pKa = 10.68 AIANTLEE19 pKa = 4.14 APATQSRR26 pKa = 11.84 RR27 pKa = 11.84 PRR29 pKa = 11.84 NRR31 pKa = 11.84 RR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 SAARR39 pKa = 11.84 QPQSIQAGASMAPIAQGAMVRR60 pKa = 11.84 LRR62 pKa = 11.84 EE63 pKa = 4.0 PSLRR67 pKa = 11.84 TAGGVTVLTHH77 pKa = 6.43 SEE79 pKa = 4.22 LSTEE83 pKa = 4.12 LAVTNAIVVSSEE95 pKa = 3.45 LVMPFTMGTWLRR107 pKa = 11.84 GVAANWSKK115 pKa = 11.55 YY116 pKa = 7.81 SLFSVRR122 pKa = 11.84 YY123 pKa = 7.48 TYY125 pKa = 10.98 LPSCPSTTSGSIHH138 pKa = 6.61 MGFQYY143 pKa = 11.56 DD144 pKa = 3.61 MADD147 pKa = 3.6 TLPVSVNQLSNLRR160 pKa = 11.84 GYY162 pKa = 11.09 VSGQVWSGSSGLCYY176 pKa = 10.29 INGTRR181 pKa = 11.84 CSDD184 pKa = 3.37 TANAITTTLDD194 pKa = 3.15 VAKK197 pKa = 10.16 LGKK200 pKa = 9.39 KK201 pKa = 8.78 WYY203 pKa = 8.93 PFKK206 pKa = 10.78 TSTDD210 pKa = 3.48 FTAAVGVIVNIATPLVPARR229 pKa = 11.84 LVIAMLDD236 pKa = 3.92 GSSSTAVSTGRR247 pKa = 11.84 LYY249 pKa = 11.25 VSYY252 pKa = 9.06 TVQLIEE258 pKa = 4.15 PTALALNNN266 pKa = 3.79
Molecular weight: 28.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.317
IPC2_protein 9.75
IPC_protein 10.452
Toseland 10.277
ProMoST 10.087
Dawson 10.496
Bjellqvist 10.248
Wikipedia 10.73
Rodwell 10.701
Grimsley 10.584
Solomon 10.555
Lehninger 10.511
Nozaki 10.306
DTASelect 10.233
Thurlkill 10.35
EMBOSS 10.701
Sillero 10.423
Patrickios 10.277
IPC_peptide 10.555
IPC2_peptide 9.297
IPC2.peptide.svr19 8.505
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1946
147
962
486.5
53.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.4 ± 1.052
2.518 ± 0.461
4.573 ± 0.692
5.653 ± 0.951
3.649 ± 0.9
7.297 ± 0.438
1.85 ± 0.609
4.111 ± 0.524
5.293 ± 0.776
8.941 ± 0.544
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.953 ± 0.325
2.826 ± 0.372
6.526 ± 1.038
3.34 ± 0.293
5.396 ± 0.683
10.175 ± 0.823
5.55 ± 1.284
7.554 ± 0.608
2.107 ± 0.283
3.289 ± 0.057
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here