Puccinia coronata f. sp. avenae

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Pucciniomycotina; Pucciniomycetes; Pucciniales; Pucciniaceae; Puccinia; Puccinia coronata; Puccinia coronata var. avenae

Average proteome isoelectric point is 7.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 25533 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N5S223|A0A2N5S223_9BASI Actin-depolymerizing factor 1 OS=Puccinia coronata f. sp. avenae OX=200324 GN=PCANC_21556 PE=3 SV=1
MM1 pKa = 7.95EE2 pKa = 5.55DD3 pKa = 3.26AQYY6 pKa = 11.26LSPLIPNFPEE16 pKa = 4.07YY17 pKa = 10.34SISGSWPEE25 pKa = 3.99EE26 pKa = 3.75SLLALDD32 pKa = 4.57SFDD35 pKa = 5.04EE36 pKa = 4.26ISEE39 pKa = 4.38ANLKK43 pKa = 9.23NLKK46 pKa = 7.96TFRR49 pKa = 11.84EE50 pKa = 4.42GAEE53 pKa = 4.34CIDD56 pKa = 4.46VEE58 pKa = 4.5MLDD61 pKa = 3.9LTPSSEE67 pKa = 4.12HH68 pKa = 5.93MLHH71 pKa = 6.76HH72 pKa = 6.65ISDD75 pKa = 4.13FEE77 pKa = 4.17MYY79 pKa = 9.55EE80 pKa = 3.96ATEE83 pKa = 4.6HH84 pKa = 5.82FWSLNLDD91 pKa = 3.98DD92 pKa = 5.86NHH94 pKa = 7.34APWTTNDD101 pKa = 2.83IYY103 pKa = 10.99MVDD106 pKa = 3.19VWLDD110 pKa = 3.49DD111 pKa = 3.99SDD113 pKa = 6.49DD114 pKa = 3.75MMSPP118 pKa = 3.63

Molecular weight: 13.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N5UHH7|A0A2N5UHH7_9BASI Uncharacterized protein OS=Puccinia coronata f. sp. avenae OX=200324 GN=PCANC_24083 PE=4 SV=1
MM1 pKa = 7.86EE2 pKa = 5.26IPHH5 pKa = 6.19SKK7 pKa = 9.91KK8 pKa = 9.79HH9 pKa = 6.05LPNTPHH15 pKa = 5.9TLIRR19 pKa = 11.84VHH21 pKa = 5.71GTLIRR26 pKa = 11.84VHH28 pKa = 5.61GTLIRR33 pKa = 11.84VHH35 pKa = 5.61GTLIRR40 pKa = 11.84VHH42 pKa = 5.61GTLIRR47 pKa = 11.84VHH49 pKa = 5.61GTLIRR54 pKa = 11.84VHH56 pKa = 5.61GTLIRR61 pKa = 11.84VHH63 pKa = 5.61GTLIRR68 pKa = 11.84VHH70 pKa = 5.61GTLIRR75 pKa = 11.84VHH77 pKa = 5.61GTLIRR82 pKa = 11.84VHH84 pKa = 5.61GTLIRR89 pKa = 11.84VHH91 pKa = 5.61GTLIRR96 pKa = 11.84VHH98 pKa = 5.61GTLIRR103 pKa = 11.84VHH105 pKa = 5.5GTFIRR110 pKa = 11.84VHH112 pKa = 5.0GTFIRR117 pKa = 11.84VQGTLIRR124 pKa = 11.84VHH126 pKa = 5.48GTFIRR131 pKa = 11.84VHH133 pKa = 5.0GTFIRR138 pKa = 11.84VQGTLIRR145 pKa = 11.84VHH147 pKa = 5.62GTFIRR152 pKa = 11.84VQGTLIRR159 pKa = 11.84VHH161 pKa = 5.48GTFIRR166 pKa = 11.84VHH168 pKa = 5.12GTLIRR173 pKa = 11.84VHH175 pKa = 5.61GTFIRR180 pKa = 11.84VNGTLIRR187 pKa = 11.84VHH189 pKa = 5.58GTLIRR194 pKa = 11.84VHH196 pKa = 5.77GTLIRR201 pKa = 11.84GTWYY205 pKa = 10.63LIRR208 pKa = 11.84VHH210 pKa = 5.82GTLIRR215 pKa = 11.84VHH217 pKa = 5.61GTLIRR222 pKa = 11.84VHH224 pKa = 5.61GTLIRR229 pKa = 11.84VHH231 pKa = 5.5GTFIRR236 pKa = 11.84VHH238 pKa = 5.12GTLIRR243 pKa = 11.84VHH245 pKa = 5.61GTLIRR250 pKa = 11.84VHH252 pKa = 5.61GTLIRR257 pKa = 11.84VHH259 pKa = 5.61GTLIRR264 pKa = 11.84VHH266 pKa = 5.61GTLIRR271 pKa = 11.84VHH273 pKa = 5.5GTFIRR278 pKa = 11.84VHH280 pKa = 5.12GTLIRR285 pKa = 11.84VHH287 pKa = 6.38

Molecular weight: 32.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

25533

0

25533

9799942

50

4782

383.8

42.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.784 ± 0.016

1.409 ± 0.007

5.101 ± 0.013

5.417 ± 0.015

3.394 ± 0.011

5.781 ± 0.016

3.113 ± 0.009

4.797 ± 0.011

5.168 ± 0.017

9.496 ± 0.019

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.939 ± 0.006

4.315 ± 0.01

7.185 ± 0.021

4.84 ± 0.014

5.675 ± 0.012

9.789 ± 0.024

6.105 ± 0.012

5.163 ± 0.011

1.213 ± 0.007

2.313 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski