Micromonospora sp. CNZ295

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales; Micromonosporaceae; Micromonospora; unclassified Micromonospora

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5816 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q8A306|A0A4Q8A306_9ACTN 16S rRNA (Cytosine967-C5)-methyltransferase OS=Micromonospora sp. CNZ295 OX=2512146 GN=EV376_3226 PE=3 SV=1
MM1 pKa = 7.35GGAAVAEE8 pKa = 4.47VATDD12 pKa = 3.71QLQVWIDD19 pKa = 3.42QDD21 pKa = 4.0LCTGDD26 pKa = 3.99GLCVQYY32 pKa = 10.98APEE35 pKa = 4.12VFEE38 pKa = 5.1FDD40 pKa = 3.14IDD42 pKa = 3.39GLAYY46 pKa = 10.67VKK48 pKa = 10.87GPDD51 pKa = 3.69GEE53 pKa = 5.29LVQSPGGRR61 pKa = 11.84VDD63 pKa = 3.56VPEE66 pKa = 4.17HH67 pKa = 6.21LRR69 pKa = 11.84LEE71 pKa = 4.75VIDD74 pKa = 4.27SAKK77 pKa = 10.05EE78 pKa = 3.91CPGEE82 pKa = 4.37CIHH85 pKa = 6.39VVRR88 pKa = 11.84GSDD91 pKa = 3.4GVEE94 pKa = 3.88VAGPEE99 pKa = 4.33AEE101 pKa = 4.31DD102 pKa = 3.36

Molecular weight: 10.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q7ZWY1|A0A4Q7ZWY1_9ACTN Uncharacterized protein DUF421 OS=Micromonospora sp. CNZ295 OX=2512146 GN=EV376_1254 PE=3 SV=1
MM1 pKa = 7.28GSVVKK6 pKa = 10.49KK7 pKa = 9.45RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.48RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.7LLRR22 pKa = 11.84KK23 pKa = 7.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84LGKK33 pKa = 10.04

Molecular weight: 4.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5816

0

5816

1889707

29

3220

324.9

34.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.263 ± 0.045

0.739 ± 0.008

6.031 ± 0.028

5.125 ± 0.035

2.638 ± 0.019

9.478 ± 0.036

2.103 ± 0.014

3.099 ± 0.019

1.622 ± 0.025

10.509 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.569 ± 0.01

1.743 ± 0.018

6.472 ± 0.035

2.605 ± 0.019

8.677 ± 0.036

4.498 ± 0.027

5.993 ± 0.025

9.167 ± 0.033

1.596 ± 0.014

2.073 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski